Page 50 - 《中国药房》2022年2期

黄丝郁金产地加工与饮片炮制工艺的一体化研究 Δ

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敖明月 ，彭 颖 ，东宝花 ，蒋云秀 ，廖宇娇 ，余凌英 ，陈志敏 ，胡昌江 （1.成都中医药大学药学院，成都
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611137；2.四川新绿色药业科技发展有限公司，成都 611900）
中图分类号 R283 文献标志码 A 文章编号 1001-0408（2022）02-0172-07
DOI 10.6039/j.issn.1001-0408.2022.02.08
摘 要 目的 优化黄丝郁金产地加工与饮片炮制的一体化工艺（以下简称“一体化炮制工艺”）。方法 按热浸法测定黄丝郁金中
醇溶性浸出物的含量，采用高效液相色谱法测定姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素的含量；在确定产地加工工艺的基
础上，以醇溶性浸出物及姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素含量的总评归一（OD）值为考察指标，切片含水量、切片厚
度、干燥温度为考察因素，以单因素实验结合星点设计-响应面法优化黄丝郁金的炮制工艺并验证，同时与按2020年版《中国药典》
（一部）方法制备的黄丝郁金传统饮片进行比较。结果 最优一体化炮制工艺为取新鲜黄丝郁金药材，置于沸水中煮5 min，于50 ℃
烘箱中干燥至切片含水量40％，切2 mm片，再置于50 ℃烘箱中干燥至饮片含水量≤15.0％。经实验验证，醇溶性浸出物及姜黄
素、去甲氧基姜黄素、双去甲氧基姜黄素含量的平均OD值（0.811 3，RSD＝2.13％）与预测值（0.848 1）的偏差为4.34％。与传统炮
制工艺所得饮片比较，一体化炮制工艺所得黄丝郁金饮片中醇溶性浸出物及姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素含量的
OD值均较高。结论 优化所得一体化炮制工艺操作简便且稳定、可行。
关键词 黄丝郁金；产地加工与饮片炮制工艺一体化；星点设计-响应面法；含量测定；总评归一值

Study on integrated technology of producing area processing and decoction pieces processing of Curcuma
longa
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AO Mingyue ，PENG Ying ，DONG Baohua ，JIANG Yunxiu ，LIAO Yujiao ，YU Lingying ，CHEN Zhimin ，
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HU Changjiang （1. College of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu
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611137，China；2. Neo-Green Pharmaceutical Co.，Ltd.，Chengdu 611900，China）
ABSTRACT OBJECTIVE To optimize the integrated technology of producing area processing and decoction pieces processing
of Curcuma longa（hereinafter refer to“integrated technology”）. METHODS The content of ethanol-soluble extract in C. longa
was determined by hot leaching method； the contents of curcumin， demethoxycurcumin and bisdemethoxycurcumin were
determined by high performance liquid chromatography. On the basis of identification of producing area processing technology，
Using overall desirability （OD） value of the contents of ethanol-soluble extract， curcumin， demethoxycurcumin and
bisdemethoxycurcumin as evaluation indexes，moisture content，slice thickness and drying temperature as factors，the integrated
technology of C. longa was optimized by single factor tests combined with central composite design-response surface method，and
the validation tests were conducted. At the same time，prepared product was compared with traditional decoction pieces prepared
according to 2020 edition of Chinese Pharmacopoeia（part Ⅰ）. RESULTS The best integrated technology was that the fresh C.
longa was boiled in boiling water for 5 min，dried at 50 ℃ to 40％ water content，cut into 2 mm thin slices，and dried at 50 ℃
until moisture content not exceeding 15.0％. After validation，The deviation between the average OD value（0.811 3，RSD＝2.13％）
and the predicted value（0.848 1）of the contents of ethanol-soluble extract，curcumin，demethoxycurcumin and bisdemethoxycurcumin
was 4.34％. OD value of the contents of ethanol-soluble extract，curcumin，demethoxycurcumin and bisdemethoxycurcumin in
decoction pieces prepared by integrated technology were all higher than those prepared by traditional technology. CONCLUSIONS
The process optimized in this study is simple，stable and feasible.
KEYWORDS Curcuma longa；integrated technology of producing area processing and decoction pieces processing；central
composite design-response surface method；content determination；overall desirability

Δ 基金项目：中国博士后科学基金面上资助项目（No.2020M6735- 黄丝郁金为姜科植物姜黄Curcuma longa L.的干燥
67XB）；四川省科技计划项目（No.20ZDYF1642）；成都中医药大学西 块根，是著名的川产道地药材，具有活血止痛、行气解
南特色中药资源重点实验室人才科研提升计划基金资助项目
郁、清心凉血、利胆退黄之功，主要用于中医治疗胸胁刺
＊硕士研究生。研究方向：中药药剂学。E-mail：2544114887@
痛、胸痹心痛、经闭痛经、乳房胀痛、热病神昏、癫痫发
qq.com
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# 通信作者：讲师，博士。研究方向：中药炮制。E-mail：chenzhi- 狂、血热吐衄、黄疸尿赤等症 。现代药理研究表明，黄
min@cdutcm.edu.cn 丝郁金具有抗炎、镇痛、抗血小板聚集、抗凝血、抗氧化、

·172 · China Pharmacy 2022 Vol. 33 No. 2 中国药房 2022年第33卷第2期