Page 53 - 2021年18期

酒仙茅的微波炮制工艺建立及与传统炮制法的比较 Δ

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李媛媛 ，王 巍，鞠成国 ，贾天柱（辽宁中医药大学药学院，辽宁 大连 116600）
中图分类号 R283 文献标志码 A 文章编号 1001-0408（2021）18-2223-07
DOI 10.6039/j.issn.1001-0408.2021.18.08

摘 要 目的：建立酒仙茅的微波炮制工艺，并与传统炮制法进行比较。方法：采用高效液相色谱法测定酒仙茅中仙茅苷、苔黑酚
葡萄糖苷、苔黑酚龙胆二糖苷的含量；在单因素实验的基础上，以加酒量、微波功率、闷润时间、微波时间为考察因素，以仙茅苷、苔
黑酚葡萄糖苷、苔黑酚龙胆二糖苷及醇溶性浸出物含量为考察指标，采用正交实验结合综合加权评分法优化微波炮制的工艺并验
证，同时比较生仙茅饮片和炮制品的含量。结果：最优微波炮制工艺为加酒量20％（生仙茅饮片质量的20％），微波功率300 W，闷
润时间 3 h，微波时间 2 min。经 3 次实验验证，仙茅苷、苔黑酚葡萄糖苷、苔黑酚龙胆二糖苷及醇溶性浸出物的平均含量分别
0.095 6％、0.723 9％、0.406 6％、10.115 3％，RSD 分别为 0.71％、0.54％、0.99％、1.44％（n＝3）；平均综合评分为 99.08 分，RSD 为
0.69％（n＝3）。除传统酒炙品中醇溶性浸出物含量外，传统酒炙品和微波炮制品中仙茅苷、苔黑酚龙胆二糖苷含量及微波炮制品
中醇溶性浸出物含量均显著高于生仙茅饮片，且微波炮制品中仙茅苷、苔黑酚龙胆二糖苷含量均显著高于传统酒炙品（P＜0.05）；两
种炮制品中苔黑酚葡萄糖苷均显著低于生仙茅饮片，但微波炮制品显著高于传统酒炙品（P＜0.05）。结论：优化所得微波炮制工艺稳
定、可行，可用于酒仙茅的炮制。
关键词 酒仙茅；微波炮制；高效液相色谱法；正交实验；含量测定

Establishment of the Microwave Processing Technology of Yellow Wine-processed Curculigo orchioides and
Comparison with Traditional Technology
LI Yuanyuan，WANG Wei，JU Chengguo，JIA Tianzhu（College of Pharmacy，Liaoning University of TCM，
Liaoning Dalian 116600，China）
ABSTRACT OBJECTIVE：To establish the microwave processing technology of yellow wine-processed Curculigo orchioides，
and compare it with traditional technology. METHODS：HPLC method was adopted to determine the contents of curculigoside，
orcinol glucoside and orcinol gentiobioside in C. orchioides. Based on the single factor tests，microwave processing technology was
optimized and validated with orthogonal test combined with comprehensive weighted scoring method，with the amount of yellow
wine，microwave power，wetting time and microwave time as factors，using the contents of curculigoside，orcinol glucoside，
orcinol gentiobioside and ethanol soluble extract as the indexes. The contents of C. orchioides decoction pieces and processed
products were compared. RESULTS：The optimal microwave processing technology included that the amount of yellow wine was
20％（the weight of C. orchioides decoction pieces was 20％），microwave power was 300 W，wetting time was 3 h，microwave
time was 2 min. After 3 times of validation tests，average contents of curculigoside，orcinol glucoside，orcinol gentiobioside and
ethanol soluble extract were 0.095 6％，0.723 9％，0.406 6％，10.115 3％，and RSD were 0.71％，0.54％，0.99％，1.44％（n＝3）.
Average comprehensive score were 99.08（RSD＝0.69％，n＝3）. Except for the content of ethanol soluble extract in traditional
wine-processed product， the contents of curculigoside and orcinol gentiobioside in traditional wine-processed product and
microwave processed product as well as the content of ethanol soluble extract in microwave processed product were all significantly
higher than C. orchioides decoction pieces；the contents of curculigoside and orcinol gentiobioside in microwave processed product
were both significantly higher than traditional wine-processed product（P＜0.05）. The contents of orcinol glucoside in 2 processed
product were significantly lower than C. orchioides decoction pieces，while the microwave processed product was significantly
higher than traditional wine-processed product（P＜0.05）. CONCLUSIONS：Optimized microwave processing technology is stable
and feasible，and can be used for the processing of yellow wine-processed C. orchioides.
KEYWORDS Yellow wine-processed Curculigo orchioides；Microwave processing technology；HPLC；Orthogonal test；Content
determination

仙茅始载于《雷公炮炙论》，为石蒜科植物仙茅Cur- 冷、筋骨痿软、腰膝冷痛、阳虚冷泻等症 。仙茅成分复
[2]
[1]
culigo orchioides Gaertn.的干燥根茎 ，其性热、味辛，有 杂，主要包括酚及酚苷类、皂苷类、木脂素类、黄酮类、生
毒，具有补肾阳、强筋骨、去寒湿的功效，可用于阳痿精 物碱类、脂肪族类及其他类成分 [3－5] 。现代药理研究显
示，仙茅具有清除氧自由基、增强免疫、延缓生殖系统衰
Δ 基金项目：辽宁省自然科学基金指导计划项目（No.20180550241）
老、抗骨质疏松、保肝、保护心血管系统等作用，可用于
＊硕士研究生。研究方向：中药炮制工艺与原理。电话：0411-
临床治疗骨质疏松症、乳腺增生、绝经后关节炎、更年
85890146。E-mail：liyuanyuan279@163.com
# 通信作者：副教授，硕士生导师。研究方向：中药炮制工艺与原 期综合征及阳痿、再生障碍性贫血、原发性高血压等疾
理。电话：0411-85890146。E-mail：jcg7092357@163.com 病 [6－8] 。

中国药房 2021年第32卷第18期 China Pharmacy 2021 Vol. 32 No. 18 ·2223 ·