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草果醇提物不同极性部位的体外抗氧化活性研究 Δ

2，3 #
1＊
2
陈石梅 ，黄比翼 ，黄锁义 （1.右江民族医学院基础医学院，广西 百色 533000；2.右江民族医学院药学院，
广西 百色 533000；3.右江民族医学院广西高校右江流域特色民族药研究重点实验室，广西 百色 533000）
中图分类号 R282 文献标志码 A 文章编号 1001-0408（2020）08-0953-04
DOI 10.6039/j.issn.1001-0408.2020.08.10

摘 要 目的：评价草果醇提物4种不同极性部位的体外抗氧化活性，为该植物中抗氧化活性化学成分的开发研究奠定基础。方
法：将草果的干燥果实粉碎后，用95％乙醇加热回流提取，经旋转蒸发浓缩、水浴蒸干后得到醇提取物浸膏；将该浸膏以水分散，
再依次用石油醚、乙酸乙酯、正丁醇进行萃取，分别合并各溶剂萃取液，并收集下层水相，再经旋转蒸发浓缩、水浴蒸干后得到石油
醚部位、乙酸乙酯部位、正丁醇部位和水部位。采用经典体外抗氧化活性试验，以 2，6-二叔丁基-4-甲基苯酚（BHT）作为阳性对
照，考察草果醇提物不同极性部位对 1，1-二苯基-2-三硝基苯肼（DPPH）自由基、超氧阴离子自由基的清除能力和对三价铁离子
3+
（Fe ）的还原能力。结果：草果醇提物4种极性部位对DPPH自由基的清除率均达到80％以上，其清除能力强弱排序为乙酸乙酯
部位＞BHT＞正丁醇部位＞石油醚部位＞水部位；对超氧阴离子自由基的清除率大部分约为30％～40％，其清除能力强弱排序
为正丁醇部位＞石油醚部位＞水部位＞乙酸乙酯部位＞BHT，但整体弱于其对DPPH自由基的清除能力；对Fe 也有一定的还原
3+
能力，其还原能力强弱排序总体上为正丁醇部位＞BHT＞乙酸乙酯部位＞石油醚部位＞水部位，但当水部位质量浓度为 4.0
μg/mL时其还原能力升至最强。结论：草果的不同极性部位均具有一定的体外抗氧化能力，但其在不同抗氧化活性试验中的抗氧
化活性能力强弱排序并不一致；乙酸乙酯部位对DPPH自由基的清除能力最强，正丁醇部位对超氧阴离子自由基的清除能力和对
Fe 的还原能力最强。
3+
关键词 草果；醇提物；极性部位；抗氧化活性；体外
Study on in vitro Antioxidant Activities of Different Polar Parts of Ethanol Extract from Amomum tsao-ko
CHEN Shimei ，HUANG Biyi ，HUANG Suoyi （1. School of Basic Medicine，Youjiang Medical University for
2，3
2
1
Nationalities， Guangxi Baise 533000， China； 2. School of Pharmacy， Youjiang Medical University for
Nationalities， Guangxi Baise 533000， China； 3. Guangxi Colleges and Universities Key Laboratory of
Characteristic Ethnic Medicine of Youjiang Valley，Youjiang Medical University for Nationalities，Guangxi
Baise 533000，China）

ABSTRACT OBJECTIVE：To evaluate in vitro antioxidant activities of 4 different polar parts of ethanol extract from Amomum
tsao-ko，and to lay a foundation for the research and development of antioxidant chemical components in the plant. METHODS：
The dried fruits of A. tsao-ko were crushed，then were hearted and reflux extracted with 95％ ethanol. The extraction fluid was
concentrated by rotary evaporation and evaporated in water bath to obtain the ethanol extract. The extract was dispersed in water，
and then extracted with petroleum ether，ethyl acetate and n-butanol organic solvents one by one. Each solvent extract was combined
and the lower water phase were collected. Finally，the petroleum ether part，ethyl acetate part，n-butanol part and water part were
obtained，after rotary evaporation concentration and water bath evaporation. Through in vitro antioxidant activity tests，using 2，
6-di-tert-butyl-4-methylphenol（BHT）as positive control，DPPH radical，superoxide anion radical scavenging ability and Fe 3 +
reducing ability of different polar parts of ethanol extract from A. tsao-ko were investigated. RESULTS：The scavenging rates of 4
polar parts of ethanol extract from A. tsao-ko on DPPH radical were all over 80％；the order of scavenging ability was ethyl acetate
part＞BHT＞n-butanol part＞petroleum ether part＞water part. Those of the 4 polar parts to superoxide anion radical were between
about 30％-40％ mostly；the order of scavenging ability was n-butanol part＞petroleum ether part＞water part＞ethyl acetate part＞
BHT；but those were weaker than their scavenging ability to DPPH radical. The polar parts of ethanol extract also had a certain
3+
reduction ability to Fe ；the order of the reduction ability was
Δ 基金项目：广西科技计划项目（No.桂科 AB18221095）；右江民
n-butanol part＞BHT＞ethyl acetate part＞petroleum ether part＞
族医学院高层次人才科研项目（No.01002018079）；右江民族医学院教
学名师培养计划项目——国家级教学名师培养计划项目（No.右医院 water part on the whole，but that of water part rose to the stron-
gest when its concentration was 4.0 μg/mL. CONCLUSIONS：
字〔2018〕98号）
＊硕士研究生。研究方向：天然药物免疫机制、卫生检验与检 The different polar parts of ethanol extract from A. tsao-ko
疫。E-mail：1439042410@qq.com have certain in vitro antioxidant capacity，but the order of
#通信作者：教授，硕士生导师。研究方向：天然药物免疫机制、天 antioxidant activity of different polar parts was not the same in
然产物化学、药物化学等。电话：0776-2827263。E-mail：huangsuoyi@ different antioxidant activity tests；ethyl acetate part has the
163.com strongest scavenging effect on DPPH radical，n-butanol part

中国药房 2020年第31卷第8期 China Pharmacy 2020 Vol. 31 No. 8 ·953 ·