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枸杞多糖抑制口腔癌细胞增殖、迁移及免疫逃逸的机制研究
Δ
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李锦宇 ，徐晓雨，王钰卓（安徽医科大学附属滁州医院/滁州市第一人民医院口腔科，安徽 滁州 239000）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2025）17-2134-07
DOI 10.6039/j.issn.1001-0408.2025.17.09
摘 要 目的 探索枸杞多糖调节T细胞免疫球蛋白黏蛋白3（TIM3）/半乳糖凝集素9（Gal-9）信号通路对口腔癌细胞增殖、迁移和
免疫逃逸的影响。方法 将人口腔癌 KB、CAL27 细胞分为对照组、低浓度枸杞多糖组（200 μg/mL）、高浓度枸杞多糖组（400
μg/mL）、pcDNA-NC 组（转染 pcDNA-NC 质粒）、pcDNA-TIM3 组（转染 pcDNA-TIM3 质粒）、高浓度枸杞多糖+pcDNA-NC 组（400
μg/mL枸杞多糖+转染pcDNA-NC质粒）、高浓度枸杞多糖+pcDNA-TIM3组（400 μg/mL枸杞多糖+转染pcDNA-TIM3质粒）。测
定细胞的增殖、迁移、侵袭能力，T细胞杀伤率，细胞上清液中干扰素γ（IFN-γ）、白细胞介素2（IL-2）水平，以及细胞中TIM3、Gal-9
mRNA表达，以及细胞中吲哚胺2，3-双加氧酶1（IDO1）、程序性死亡受体配体1（PD-L1）、TIM3、Gal-9蛋白表达。结果 与对照组
比较，低、高浓度枸杞多糖组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9 mRNA及其蛋
白表达水平均显著降低/减少（P＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著升高（P＜0.05）；与对照组、pcDNA-
NC组比较，pcDNA-TIM3组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9 mRNA及其蛋
白表达水平均显著升高/增加（P＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著降低（P＜0.05）；与高浓度枸杞多糖
组、高浓度枸杞多糖+pcDNA-NC组比较，高浓度枸杞多糖+pcDNA-TIM3组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及
IDO1、PD-L1蛋白和TIM3、Gal-9 mRNA及其蛋白表达水平均显著升高/增加（P＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2
水平均显著降低（P＜0.05）。结论 枸杞多糖可能通过抑制TIM3/Gal-9信号通路来抑制口腔癌细胞增殖、迁移和免疫逃逸。
关键词 枸杞多糖；口腔癌；T细胞免疫球蛋白黏蛋白3；半乳糖凝集素9；增殖；迁移；免疫逃逸
Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation， migration and
immune escape of oral cancer cells
LI Jinyu，XU Xiaoyu，WANG Yuzhuo（Dept. of Stomatology， the Affiliated Chuzhou Hospital of Anhui Medical
University/Chuzhou First People’s Hospital， Anhui Chuzhou 239000， China）
ABSTRACT OBJECTIVE To explore the effects of Lycium barbarum polysaccharides on the proliferation， migration， and
immune escape of oral cancer cells by regulating the T cell immunoglobulin and mucin domain-containing protein 3 （TIM3）/
galectin-9 （Gal-9） signaling pathway. METHODS Human oral cancer cells KB and CAL27 were assigned to control group， L.
barbarum polysaccharides low-concentration group （200 μg/mL）， L. barbarum polysaccharides high-concentration group （400
μg/mL）， pcDNA-NC group （transfection of pcDNA-NC plasmid）， pcDNA-TIM3 group （transfection of pcDNA-TIM3 plasmid），
high concentration of L. barbarum polysaccharides+pcDNA-NC group （400 μg/mL L. barbarum polysaccharides + transfection of
pcDNA-NC plasmid）， and high concentration of L. barbarum polysaccharides+pcDNA-TIM3 group （400 μg/mL L. barbarum
polysaccharides + transfection of pcDNA-TIM3 plasmid）. The proliferation， migration and invasion abilities of the cells， T cell
killing rate as well as the levels of interferon-γ （IFN-γ） and interleukin-2 （IL-2） in the cell supernatant were measured. mRNA
expressions of TIM3 and Gal-9 and protein expressions of indoleamine 2，3-dioxygenase 1 （IDO1）， programmed death-ligand 1
（PD-L1）， TIM3 and Gal-9 in the cells were also determined. RESULTS Compared with control group， the clone formation rate，
scratch healing rate， the number of invasive cells， protein expressions of IDO1 and PD-L1， mRNA and protein expressions of
TIM3 and Gal-9 in both cell types of L. barbarum polysaccharide low- and high-concentration groups were decreased significantly
（P＜0.05）， while the proliferation inhibition rate， T cell killing rate， and the levels of IFN-γ and IL-2 were significantly increased
（P＜0.05）. Compared with control group and pcDNA-NC group， the clone formation rate， scratch healing rate， the number of
invasive cells， and protein expressions of IDO1 and PD-L1， mRNA and protein expressions of TIM3 and Gal-9 in both cell types
of the pcDNA-TIM3 group were all significantly increased （P＜0.05）， while the proliferation inhibition rate， T cell killing rate，
IFN- γ and IL-2 levels were significantly decreased （P＜0.05）. Compared with L. barbarum polysaccharides high-concentration
group and high concentration of L. barbarum polysaccharides+pcDNA-NC group， the clone formation rate， scratch healing rate，
the number of invasive cells， and protein expressions of IDO1
Δ 基金项目 安徽省自然科学基金项目（No.2408085QB059） and PD-L1， mRNA and protein expressions of TIM3 and Gal-
＊第一作者 主治医师。研究方向：口外、口内修复。E-mail： 9 in both cell types of high concentration of L. barbarum
18326905112@163.com polysaccharides+pcDNA-TIM3 group were significantly
· 2134 · China Pharmacy 2025 Vol. 36 No. 17 中国药房 2025年第36卷第17期
