Page 77 - 《中国药房》2025年14期

化学计量学结合加权TOPSIS模型评价不同产地野鸦椿质量 Δ

1， 2 #
高雨秋 1， 2＊ ，郑 帅 ，于 雪 ，邹桂华 ，张 凯 （1.牡丹江医科大学附属红旗医院药学部，黑龙江 牡丹江
3
1， 2
1， 2
157000；2.牡丹江医科大学第一临床医学院，黑龙江 牡丹江 157000；3.牡丹江医科大学药学院，黑龙江 牡丹江
157011）

中图分类号 R917；R284.1 文献标志码 A 文章编号 1001-0408（2025）14-1755-05
DOI 10.6039/j.issn.1001-0408.2025.14.11

摘 要 目的 评价不同产地野鸦椿的质量。方法 以槲皮素为内参物，计算没食子酸、原儿茶酸、鞣花酸、异槲皮苷、紫云英苷和
芹菜素的相对校正因子，以熊果酸为内参物计算野鸦椿酸、齐墩果酸、豆甾醇和β-谷甾醇的相对校正因子，采用一测多评（QAMS）
法测定18批野鸦椿样品中12个成分的含量，并与外标法测定结果进行比较；同时检测其水溶性浸出物、醇溶性浸出物、总灰分、酸
不溶性灰分含量。采用主成分分析、正交偏最小二乘法-判别分析（OPLS-DA）和加权逼近理想解排序（TOPSIS）法综合评价其质
量。结果 18 批样品中 12 个成分的 QAMS 法与外标法测定结果差异不显著，但不同批次样品的含量差异较大。主成分分析和
OPLS-DA 结果均显示，S1～S7、S8～S12、S13～S18 分别聚为一类；7 个关键特征性成分的变量重要性投影值＞1，分别为野鸦椿
酸、熊果酸、原儿茶酸、芹菜素、β-谷甾醇、异槲皮苷和齐墩果酸；加权TOPSIS法结果显示，18批样品的相对贴近度为0.283 5～0.644 1，
其中重庆奉节县产野鸦椿的质量最优。结论 所建含量测定方法准确可行，结合化学计量学及加权TOPSIS法可用于野鸦椿的质
量评价。
关键词 野鸦椿；一测多评法；化学计量学；加权TOPSIS法；质量评价

Quality evaluation of Euscaphis japonica from different habitats using chemometrics combined with
weighted TOPSIS model
GAO Yuqiu ，ZHENG Shuai ，YU Xue ，ZOU Guihua ，ZHANG Kai （1. Dept. of Pharmacy， Hongqi
1， 2
3
1， 2
1， 2
1， 2
Hospital Affiliated to Mudanjiang Medical University， Heilongjiang Mudanjiang 157000， China；2. First Clinical
Medical College， Mudanjiang Medical University， Heilongjiang Mudanjiang 157000， China；3. College of
Pharmacy， Mudanjiang Medical University， Heilongjiang Mudanjiang 157011， China）
ABSTRACT OBJECTIVE To evaluate the quality of Euscaphis japonica from different habitats. METHODS The relative
correction factors of gallic acid， protocatechuic acid， ellagic acid， isoquercitrin， astragalin and apigenin were calculated with
quercetin as the internal reference； the relative correction factors of euscaphic acid， oleanolic acid， stigmasterol and β-sitosterol
were also calculated with ursolic acid as the internal reference. The contents of 12 components in 18 batches of samples were
calculated by QAMS method and were compared with external standard method. At the same time， the contents of water-soluble
extract， alcohol-soluble extract， total ash and acid-insoluble ash were detected. The quality of E. japonica was evaluated by
principal component analysis （PCA）， orthogonal partial least squares-discriminant analysis （OPLS-DA）， and weighted technique
for order preference by similarity to ideal solution （TOPSIS） method. RESULTS There was no significant difference between the
results of QAMS method and external standard method for the 12 components in the 18 batches of samples. However， notable
content variations were observed among different batches of samples. The results of PCA and OPLS-DA showed that S1-S7， S8-
S12， and S13-S18 were clustered into one category respectively. Seven key characteristic components variable importance in
projection values ＞1， euscaphic acid， ursolic acid， protocatechuic acid， apigenin， β-sitosterol， isoquercitrin， and oleanolic acid，
respectively. The analysis results of the weighted TOPSIS method revealed that the relative closeness for evaluating the quality of 18
batches of samples ranged from 0.283 5 to 0.644 1， with the samples of E. japonica from Fengjie， Chongqing， demonstrating the
highest quality. CONCLUSIONS The established method is accurate and feasible， which can be used for the quality evaluation of
E. japonica combined with chemometrics and weighted
Δ 基金项目 黑龙江省医药卫生科研课题（No.2020-410）
＊第一作者 主管药师，硕士。研究方向：药物质量评价、医院药 TOPSIS model.
学。E-mail：tbvuxc@163.com KEYWORDS Euscaphis japonica； QAMS； chemometrics；
# 通信作者 主管药师，硕士。研究方向：药物质量评价、临床药 weighted TOPSIS method； quality evaluation
学。E-mail：ell953@163.com

中国药房 2025年第36卷第14期 China Pharmacy 2025 Vol. 36 No. 14 · 1755 ·