Page 40 - 《中国药房》2025年6期

三七丹参片治疗非酒精性脂肪性肝病的作用机制研究
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雷雨田 ，冯 丹，陈新利，杨 媛，吴 晖（昆明医科大学第一附属医院临床药学中心，昆明 650032）
中图分类号 R965；R285.5 文献标志码 A 文章编号 1001-0408（2025）06-0674-06
DOI 10.6039/j.issn.1001-0408.2025.06.06

摘 要 目的 探讨三七丹参片治疗非酒精性脂肪性肝病（NAFLD）的潜在机制。方法 采用网络药理学方法，挖掘三七丹参片治
疗NAFLD的核心靶点，并进行基因本体（GO）功能及京都基因和基因组数据库（KEGG）通路富集分析。基于网络药理学结果，以
SD大鼠为对象，以高脂饲料喂养构建NAFLD模型，考察三七丹参片对NAFLD大鼠肝组织脂滴空泡、脂质蓄积等病理改变以及脂
质代谢、炎症反应、氧化应激指标的影响。结果 共筛选出三七丹参片治疗NAFLD的核心靶点20个，主要涉及生物调节、细胞膜、
结合等GO功能，并富集于炎症反应、氧化应激、脂质代谢相关信号通路。动物实验显示，与模型组相比，三七丹参片低、中、高剂
量组和阳性对照药（辛伐他汀）组大鼠肝组织内脂滴空泡明显减少，脂滴数量明显减少且颜色变浅；其肝组织中总胆固醇、甘油三
酯（三七丹参片中剂量组除外）、天冬氨酸转氨酶、丙氨酸转氨酶、肿瘤坏死因子α（三七丹参片低剂量组除外）、白细胞介素17（三
七丹参片各剂量组除外）、丙二醛（三七丹参片低剂量组除外）含量均显著降低，超氧化物歧化酶含量均显著升高（P＜0.01或P＜
0.05）。结论 三七丹参片可通过影响炎症反应、氧化应激、脂质代谢等相关指标含量来发挥抗炎、抗氧化、调节脂质代谢的作用，
从而改善大鼠的NAFLD。
关键词 三七丹参片；非酒精性脂肪性肝病；炎症反应；氧化应激；脂质代谢

Effect and mechanism of Sanqi danshen tablets in the treatment of non-alcoholic fatty liver disease
LEI Yutian，FENG Dan，CHEN Xinli，YANG Yuan，WU Hui（Dept. of Clinical Pharmacy Center， the First
Affiliated Hospital of Kunming Medical University， Kunming 650032， China）

ABSTRACT OBJECTIVE To investigate the potential mechanism of Sanqi danshen tablets in the treatment of non-alcoholic
fatty liver disease （NAFLD）. METHODS Core targets of Sanqi danshen tablets in the treatment of NAFLD were explored by
network pharmacological methods. Gene ontology （GO） functional enrichment analysis and Kyoto Encyclopedia of Genes and
Genomes （KEGG） pathway enrichment analysis were also performed. Based on the results obtained from network pharmacological
studies， using SD rats as subjects， the NAFLD model was induced by feeding them high-fat diet. The effects of Sanqi danshen
tablets on pathological changes such as lipid droplet vacuoles and lipid accumulation in the liver tissue of NAFLD rats， as well as
its impact on relative indicators of lipid metabolism， inflammatory responses and oxidative stress， were investigated. RESULTS A
total of 20 core targets for the treatment of NAFLD with Sanqi danshen tablets were screened， primarily involved in GO functions
such as biological regulation， cellular membrane and binding， and enriched in signaling pathways related to inflammatory
responses， oxidative stress and lipid metabolism. Compared with the model group， lipid droplet vacuoles were reduced significantly
in low-dose， medium-dose， high-dose groups of Sanqi danshen tablets and positive control （simvastatin） group， the number of
lipid droplets decreased significantly and the color became lighter. The contents of total cholesterol， triglyceride （except for medium-
dose group of Sanqi danshen tablets）， aspartate transaminase， alanine transaminase， tumor necrosis factor-α （except for low-dose
group of Sanqi danshen tablets）， interleukin-17 （except for Sanqi danshen tablets groups） and malondialdehyde （except for low-
dose group of Sanqi danshen tablets） in liver tissue were significantly decreased， while the content of superoxide dismutase was
significantly increased （P＜0.01 or P＜0.05）. CONCLUSIONS Sanqi danshen tablets exert anti-inflammatory， antioxidant and
lipid metabolism regulating effects by influencing the levels of
Δ 基金项目 云南省科技厅科技计划项目基础研究专项（No.
202301AT070152）；云南省科技厅科技计划项目重大科技专项（生物医 inflammation， oxidative stress and lipids metabolism-related
药）（No.2019ZF005）；中华国际医学交流基金会-中华医学会临床药学 indicators， thereby improving NAFLD in rats.
分会临床药学科研基金项目（No.Z-202146-2101-2023） KEYWORDS Sanqi danshen tablets； non-alcoholic fatty
＊第一作者 药师，硕士研究生。研究方向：临床药学、药理学。E-
liver disease； inflammatory response； oxidative stress； lipid
mail：2079957968@qq.com
metabolism
# 通信作者 主任药师，博士生导师，硕士。研究方向：临床药学、
药理学。E-mail：kyz-ggyx@163.com

· 674 · China Pharmacy 2025 Vol. 36 No. 6 中国药房 2025年第36卷第6期