Page 43 - 《中国药房》2023年18期

蜜糠炒苍术炮制工艺优选及其抗胃溃疡作用研究
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朱文婷 1， 2＊ ，陆美霞 ，黄瑶洁 ，刘志伟 ，丁 燕 ，施林峰 ，叶喜德 （1.江西中医药大学附属医院药学部，南昌
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330006；2.江西中医药大学药学院，南昌 330004）
中图分类号 R965；R283 文献标志码 A 文章编号 1001-0408（2023）18-2213-06
DOI 10.6039/j.issn.1001-0408.2023.18.08
摘 要 目的 优选蜜糠炒苍术的炮制工艺，并对其炮制前后的抗胃溃疡作用进行比较。方法 采用L9 （3）正交实验设计，结合熵
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权逼近理想解排序（TOPSIS）模型，以苍术酮、β-桉叶醇、白术内酯Ⅲ、苍术素含量的综合评分为评价指标，以辅料与药材比例、炒
制温度、炒制时间为考察因素，优选蜜糠炒苍术的炮制工艺并验证。以无水乙醇灌胃建立胃溃疡小鼠模型，以复方氢氧化铝片为
阳性对照，以血清炎症因子[白细胞介素2（IL-2）、IL-6、肿瘤坏死因子α（TNF-α）]含量、溃疡指数和胃溃疡抑制率为评价指标，比较
生苍术和蜜糠炒苍术的抗胃溃疡作用。结果 蜜糠炒苍术的最优炮制工艺为辅料与药材比例3∶10（g/g）、炮制温度140 ℃、炒制时
间 4 min。3 次验证实验结果显示，最优工艺炮制所得蜜糠炒苍术中苍术酮等 4 种成分的含量较为稳定（RSD 为 3.47%～5.80%，
n＝3），综合评分为95.53%～95.89%（RSD＝0.21%，n＝3）。生苍术和蜜糠炒苍术均可不同程度地升高胃溃疡模型小鼠血清IL-2
含量，降低其血清IL-6、TNF-α含量，蜜糠炒苍术可显著降低其溃疡指数（P＜0.05或P＜0.01）；蜜糠炒苍术对上述指标的改善作用普
遍优于同剂量生苍术（P＜0.05 或 P＜0.01）；低、中、高剂量生苍术和蜜糠炒苍术对小鼠胃溃疡的抑制率分别为 9.18%、19.30%、
30.70%和50.32%、61.39%、53.16%。结论 优选出的蜜糠炒苍术炮制工艺稳定、可行，且苍术经蜜糠炒制后抗胃溃疡的作用有所
增强。
关键词 苍术；蜜糠；炮制工艺；正交实验设计法；熵权法；TOPSIS模型；胃溃疡；药效学

Study on the optimization of processing technology of honey bran-fried Atractylodis Rhizoma and its anti-
gastric ulcer effect
ZHU Wenting ，LU Meixia ，HUANG Yaojie ，LIU Zhiwei ，DING Yan ，SHI Linfeng ，YE Xide（1. Dept. of
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Pharmacy， the Affiliated Hospital of Jiangxi University of Chinese Medicine， Nanchang 330006， China；
2. School of Pharmacy， Jiangxi University of Chinese Medicine， Nanchang 330004， China）
ABSTRACT OBJECTIVE To optimize the processing technology of honey bran-fried Atractylodis Rhizoma， and to compare the
anti-gastric ulcer effect before and after processing. METHODS Combing with entropy-weight and technique for order preference
by similarity to ideal solution model， L9 （3） orthogonal experiment design was adopted to optimize the processing technology of
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honey bran-fried Atractylodis Rhizoma using the comprehensive score of the contents of atractylone， β-cineole， atractylenolide Ⅲ
and atractylodine as evaluation index， using the ratio of excipients to medicine， frying temperature and frying time as factors. The
validation tests were conducted. The gastric ulcer model of mice was induced by intragastrical administration of anhydrous ethanol；
using Compound aluminum hydroxide tablet as positive control， anti-gastric ulcer effect of Atractylodis Rhizoma was compared
with that of honey bran-fried Atractylodis Rhizoma using the contents of serum inflammatory factors [interleukin-2 （IL-2）， IL-6，
tumor necrosis factor-α （TNF-α）]， ulcer index and inhibitory rate of gastric ulcer as evaluation indexes. RESULTS The optimal
processing technology of honey bran-fried Atractylodis Rhizoma was as follows：ratio of adjuvant and medicinal materials of 3∶10
（g/g）， frying temperature at 140 ℃ and frying time of 4 min. Results of 3 validation tests showed that the contents of 4
components （including atractylone）， in honey bran-fried Atractylodis Rhizoma processed by the optimal technology kept stable
（RSDs were 3.47%-5.80%， n＝3）； the comprehensive scores were 95.53%-95.89% （RSD＝0.21%， n＝3）. Atractylodis Rhizoma
and honey bran-fried Atractylodis Rhizoma could increase the serum content of IL-2 in mice， but reduce serum contents of IL-6 and
TNF-α to varying degrees； honey bran-fried Atractylodis Rhizoma could significantly decrease its ulcer indexes （P＜0.05 or P＜
0.01）； the improvement effect of honey bran-fried Atractylodis Rhizoma on the above indicators was generally better than that of
the same dosage of Atractylodis Rhizoma （P＜0.05 or P＜
Δ 基金项目 江西省中医药管理局科技计划项目（No.2021B652）； 0.01）. The inhibitory rates of low-dose， medium-dose and
江西省中医药中青年骨干人才（第一批）培养计划（No. 赣中医药科教 high-dose Atractylodis Rhizoma and honey bran-fried
字〔2020〕2号） Atractylodis Rhizoma to gastric ulcer in mice were 9.18%，
＊第一作者 主管中药师，硕士。研究方向：中药炮制。E-mail：
19.30%， 30.70%， and 50.32%， 61.39%， 53.16%，
1459523369@qq.com
respectively. CONCLUSIONS The optimal processing
# 通信作者 教授，博士生导师，博士。研究方向：中药炮制。E-
mail：552376722@qq.com technology of honey bran-fried Atractylodis Rhizoma is stable

中国药房 2023年第34卷第18期 China Pharmacy 2023 Vol. 34 No. 18 · 2213 ·