Page 58 - 中国药房2023年10期
P. 58
玉屏风加味鼻喷剂对 AR 模型大鼠鼻黏膜损伤的保护作用及机
制研究
Δ
＊
习 媛 ，贺心宁，刘玉崟，张 娜，刘 婷，梁方琪，田 理（成都中医药大学附属医院耳鼻喉科，成都
#
610032）
中图分类号 R965；R276.1 文献标志码 A 文章编号 1001-0408（2023）10-1204-07
DOI 10.6039/j.issn.1001-0408.2023.10.10
摘 要 目的 研究玉屏风加味鼻喷剂（YPF+）对变应性鼻炎（AR）模型大鼠鼻黏膜损伤的保护作用及潜在机制。方法 以卵清蛋
白（OVA）诱导建立大鼠AR模型。将造模成功的大鼠随机分为模型组、YPF+组（每侧50 μg，每天2次）和阳性对照组（糠酸莫米松
鼻喷雾剂，每侧50 μg，每天1次），并设空白组，每组10只。各药物组大鼠给予相应药物，空白组和模型组大鼠给予等量生理盐水，
连续 4 周。末次给药结束 30 min 后，记录各组大鼠的行为评分，观察其鼻黏膜组织的病理改变；检测其鼻黏膜组织中活性氧
（ROS）水平，核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）、胱天蛋白酶1（caspase-1）、gasdermin D（GSDMD）蛋白及mRNA的
表达水平，以及血清中白细胞介素1β（IL-1β）、IL-18含量。结果 与空白组比较，模型组大鼠鼻黏膜组织结构紊乱，炎症细胞浸润
严重，并可见固有层血管扩张；其行为学评分和病理评分，鼻黏膜组织中ROS水平，NLRP3、caspase-1、GSDMD蛋白及其mRNA的
表达水平，以及血清IL-1β、IL-18含量均显著升高（P＜0.05）。与模型组比较，各药物组大鼠鼻黏膜损伤症状均有不同程度改善，
上述指标均显著降低（P＜0.05）。结论 YPF+可改善大鼠鼻黏膜损伤，减轻其喷嚏、鼻痒、鼻涕等AR症状，这种作用可能与其减少
鼻黏膜ROS产生、下调NLRP3/caspase-1/GSDMD通路有关。
关键词 玉屏风加味鼻喷剂；变应性鼻炎；NLRP3/caspase-1/GSDMD通路；活性氧
Study on protective effect and mechanism of Modified Yupingfeng nasal spray on nasal mucosal injury in
AR model rats
XI Yuan，HE Xinning，LIU Yuyin，ZHANG Na，LIU Ting，LIANG Fangqi，TIAN Li （Dept. of
Otorhinolaryngology， the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine， Chengdu
610032， China）
ABSTRACT OBJECTIVE To study protective effect and potential mechanism of Modified yupingfeng nasal spray （YPF+） on
nasal mucosal injury in allergic rhinitis （AR） model rats. METHODS AR model was induced by ovalbumin （OVA） and randomly
divided into model group， YPF+group （50 µg/side，twice a day）， positive control group （Mometasone furoate aqueous nasal spray，
50 µg/side，once a day）； the blank group was set up， with 10 rats in each group. Administration groups were given relevant
medicine， and blank group and model group were given equivalent normal saline for consecutive 4 weeks. Thirty minutes after last
medication， the behavioral scores of rats were recorded， and the pathological changes of their nasal mucosa tissue were observed.
The level of reactive oxygen species （ROS） in nasal mucosa tissue was detected. The protein and mRNA expressions of nucleotide-
binding oligomerization domain-like receptor protein 3 （NLRP3），caspase-1，gasdermin D （GSDMD） were detected； the contents of
interleukin-1β （IL-1β） and IL-18 in serum were also determined. RESULTS Compared with blank group， in model group， the
nasal mucosa tissue structure was disordered， inflammatory cells infiltrated seriously， and lamina propria vascular dilation was
visible； its behavioral score and pathological score， the level of ROS， protein and mRNA expressions of NLRP3， caspase-1 and
GSDMD， serum contents of IL-1β and IL-18 in nasal mucosa tissue were increased significantly （P＜0.05）. Compared with model
group， the symptoms of nasal mucosal injury in rats of each drug group were improved to varying degrees， and the above
indicators were significantly reduced （P＜0.05）. CONCLUSIONS YPF+ may improve nasal mucosal injury of rats， relieve AR
symptoms such as sneezing， itchy nose， runny nose， the mechanism of which may be associated with reducing the production of
ROS in nasal mucosa and downregulating NLRP3/caspase-1/
Δ 基金项目 四川省科技计划项目（No.2021YFS0036）；四川省中
GSDMD pathway.
医药院士后备人才培养项目（No.2100601） KEYWORDS Modified yupingfeng nasal spray； allergic
＊第一作者 医师，硕士研究生。研究方向：中医药防治变态反应
性耳鼻咽喉疾病的基础。E-mail：2418079767@qq.com rhinitis； NLRP3/caspase-1/GSDMD pathway； reactive oxygen
species
# 通信作者 主任医师，教授，博士生导师。研究方向：中医药防治
变态反应性耳鼻咽喉疾病。E-mail：ctcmdan@stu.cdutcm.edu.cn
· 1204 · China Pharmacy 2023 Vol. 34 No. 10 中国药房 2023年第34卷第10期
