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不同产地独一味总黄酮的质量评价
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陈瑞鑫 ，蒋运斌 ，陈文莉 ，康点点 ，李 蕊 ，蒋桂华 （1.成都中医药大学药学院/西南特色中药资源国家重
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点实验室，成都 611137；2. 西南大学药学院中医药学院，重庆 400715；3. 成都中医药大学国学院，成都
611137）
中图分类号 R917 文献标志码 A 文章编号 1001-0408（2023）04-0419-05
DOI 10.6039/j.issn.1001-0408.2023.04.07

摘 要 目的 评价不同产地独一味总黄酮的质量。方法 采用渗漉法提取、聚酰胺柱纯化S1～S15批次不同产地的独一味总黄
酮。采用紫外分光光度法测定独一味总黄酮含量，并计算纯度。通过《中药色谱指纹图谱相似度评价系统（2012版）》软件建立15
批独一味总黄酮的高效液相色谱指纹图谱和对照指纹图谱，并分析相似度。采用聚类分析、主成分分析、正交偏最小二乘法-判别
分析评价15批独一味总黄酮的质量，并分析影响质量的主要成分。结果 15批独一味总黄酮纯度为55.82%～94.12%，平均值为
77.72%；其指纹图谱中共确定了 5 个共有峰，3 号峰为木犀草苷，与对照指纹图谱的相似度在 0.925～1.000 之间；聚类分析将 S1、
S3～S9聚为第1类，为青海省和西藏自治区样品；S14、S15聚为第2类，均为云南省样品；S10～S13聚为第3类，均为四川省样品；
S2聚为第4类；主成分分析得到第1类和第4类质量较优；正交偏最小二乘法-判别分析筛选出2、3、5号峰为引起质量差异的主要
成分。结论 青海省和西藏自治区的独一味总黄酮质量较好。
关键词 独一味；总黄酮；指纹图谱；高效液相色谱法；化学计量学；质量评价

Quality evaluation of total flavonoids of Lamiophlomis rotata from different producing areas
CHEN Ruixin ，JIANG Yunbin ，CHEN Wenli ，KANG Diandian ，LI Rui ，JIANG Guihua （1. School of
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Pharmacy， Chengdu University of Traditional Chinese Medicine/State Key Laboratory of Southwestern Chinese
Medicine Resources， Chengdu 611137， China；2. School of Pharmaceutical Sciences and Chinese Medicine，
Southwest University， Chongqing 400715， China；3. School of Chinese Classics， Chengdu University of
Traditional Chinese Medicine， Chengdu 611137， China）
ABSTRACT OBJECTIVE To evaluate the quality of total flavonoids of Lamiophlomis rotata from different producing areas.
METHODS Total flavonoids of S1-S15 batches of L. rotata from different producing areas were extracted by percolation and
purified by polyamide column. The content of total flavonoids was determined by UV spectrophotometry， and its purity was
calculated. HPLC fingerprint chromatograms and control fingerprint of total flavonoids from 15 batches of L. rotata were established
with Similarity Evaluation System of Chromatographic Fingerprint of TCM （2012 version）. Their similarities were analyzed. Cluster
analysis， principal component analysis and orthogonal partial least squares-discriminant analysis were used to evaluate the quality of
total flavonoids from 15 batches of L. rotata， and the main components which affected the quality of total flavonoids were
analyzed. RESULTS The purities of total flavonoids from 15 batches of L. rotata were 55.82%-94.12%， with an average value of
77.72%； a total of five common peaks were identified in the fingerprint， and No. 3 peak was luteolin； the similarities between the
fingerprint of each batch of samples and the control fingerprint were 0.925-1.000. By cluster analysis， S1 and S3-S9 were clustered
into the first class， which were samples from Qinghai Province and Tibet Autonomous Region. S14 and S15 were clustered into the
second class， which were samples from Yunnan Province. S10-S13 were clustered into the third class， which were all samples from
Sichuan Province. S2 was clustered into the fourth class. The principal component analysis showed that the qualities of samples
from the first and fourth classes were better； peaks 2， 3 and 5 were identified as the main components that caused the differences
among different batches of samples by orthogonal partial least squares-discriminant analysis. CONCLUSIONS The qualities of total
flavonoids of L. rotata from Qinghai Province and Tibet Autonomous Region are better.
KEYWORDS Lamiophlomis rotata； total flavonoids；
Δ 基金项目 国家自然科学基金资助项目（No.82104528）；国家重
fingerprint； HPLC； chemometrics； quality evaluation
点研发计划项目（No.2019YFC1712302）；重庆市自然科学基金面上项
目（No.cstc2020jcyj-msxmX0146）
＊ 第一作者 硕 士 研 究 生 。 研 究 方 向 ：中 药 鉴 定 。 E-mail： 独一味为唇形科植物独一味 Lamiophlomis rotata
crx0105@163.com
（Benth.）Kudo 的干燥地上部分，藏药名为“达巴”，系藏
# 通信作者 教授，博士生导师，博士。研究方向：中药品种、质量
与资源开发。E-mail：11469413@qq.com 族习用药材，也常作为中药使用。2020年版《中国药典》

中国药房 2023年第34卷第4期 China Pharmacy 2023 Vol. 34 No. 4 · 419 ·