Page 48 - 《中国药房》2022年14期

不同产地加工方法对香附质量的影响 Δ

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纪 亮 1，2＊ ，雷敬卫 ，张维方 ，贾 豪 ，李莹莹 ，王心乐（1.河南中医药大学药学院，郑州 450046；2.中国
中医科学院中药研究所，北京 100700；3.河南省中药质量控制与评价工程技术研究中心，郑州 450046）
中图分类号 R932 文献标志码 A 文章编号 1001-0408（2022）14-1706-06
DOI 10.6039/j.issn.1001-0408.2022.14.08

摘 要 目的 探究蒸制后晒干、煮制后晒干和直接晒干的不同产地加工方法对香附质量的影响，并从化学成分角度探索香附的
最佳产地加工方法。方法 采用高效液相色谱（HPLC）法，结合《中药色谱指纹图谱相似度评价系统（2012版）》建立香附的指纹图
谱，并进行相似度评价，确定共有峰；结合聚类分析（CA）、主成分分析（PCA）、偏最小二乘法-判别分析（PLS-DA）对不同产地加工
方法处理的香附进行区分比较。利用HPLC法测定香附药材中香附烯酮、α-香附酮、木犀草素和阿魏酸的含量。结果 不同产地加
工方法处理的香附 HPLC 指纹图谱共有 22 个共有峰，相似度均大于 0.9，其中 16 号和 20 号共有峰分别是香附烯酮和α-香附酮。
CA结果显示，煮制4、8、12 min的香附样品聚为一类，其余样品聚为一类；PCA结果显示，蒸制10、15 min和煮制2 min的香附样品
综合得分最高，同时蒸制处理对样品的综合得分影响较小，而煮制处理影响较大，在煮制 2 min 后样品综合得分急剧下降。
PLS-DA结果显示，HPLC指纹图谱中20号峰（α-香附酮）、16号峰（香附烯酮）、22号峰、17号峰和21号峰的变量重要性投影（VIP）
值均＞1。含量测定结果显示，不同产地加工方法所得样品中α-香附酮、香附烯酮和木犀草素含量存在明显差异，随着蒸、煮时间
的增加，α-香附酮、香附烯酮和木犀草素的含量均呈明显的降低趋势，且煮制对其影响极大。结论 煮制时间过长可极大破坏香附
中的化学成分，蒸制10、15 min或煮制2 min为较佳的加工方法。
关键词 香附；产地加工；指纹图谱；含量测定

Effects of different habitat processing methods on the quality of Cyperus rotundus
JI Liang ，LEI Jingwei ，ZHANG Weifang ，JIA Hao ，LI Yingying ，WANG Xinle（1. School of Pharmacy，
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Henan University of Chinese Medicine，Zhengzhou 450046，China；2. Institute of Chinese Materia Medica，
China Academy of Chinese Medical Sciences， Beijing 100700， China； 3.Henan Provincial Engineering
Technology Research Center of Traditional Chinese Medicine Quality Control and Evaluation，Zhengzhou
450046，China）
ABSTRACT OBJECTIVE To investigate the effects of different habitat processing methods on the quality of Cyperus rotundus，
such as sun-drying after steaming，sun-drying after boiling and direct sun-drying，and to investigate the optimal habitat processing
method of C. rotundus from the perspective of chemical component. METHODS The fingerprint of C. rotundus was established by
high performance liquid chromatography（HPLC）combined with the Similarity Evaluation System for Chromatographic Fingerprint
of TCM（2012 edition）. The similarity evaluation was conducted to determine the common peak. Cluster analysis（CA）combined
with principal component analysis（PCA）and partial least squares-discriminant analysis（PLS-DA）was used to differentiate and
compare C. rotundus treated by different habitat processing methods. And the contents of cyperrenone，α-cyperone，luteolin and
ferulic acid in C. rotundus were determined by HPLC. RESULTS There were 22 common peaks in the HPLC fingerprints of C.
rotundus reated by different habitat processing methods；their similarities were higher than 0.9；common peak 16 and common peak
20 were cyperrenone and α-cyperone. CA results showed that C. rotundus boiled for 4，8 and 12 min were clustered into one
category，the rest of the samples clustered into one category. PCA results showed that comprehensive score of C. rotundus steamed
for 10 and 15 min and boiled for 2 min were the highest. The steaming treatment had small effect on comprehensive score of
samples，while the boiling treatment had a great effect on the quality of the samples；the overall score of samples decreased sharply
after boiling for 2 min. Results of PLS-DA showed that variable importance projection（VIP）of peak 20（α-cyperone），peak 16
（cyperrenone），peak 22，peak 17 and peak 22 in HPLC fingerprints were all higher than 1. The results of content determination
showed that there was significant difference in the contents of α-cyperone，cyperrenone and luteolin in samples treated by different
habitat processing methods.With the increase of steaming and boiling time，the contents of α-cyperone，cyperrenone and luteolin
showed a significant downward trend，and boiling had a great impact on them. CONCLUSIONS Too long boiling treatment greatly
destroys the chemical composition of C. rotundus， and
Δ 基金项目 国家重点研发计划项目（No.2018YFC1707000）；2019
steaming for 10，15 min or boiling for 2 min is the optimal
年河南中医药大学研究生学术创新基金项目
processing method.
＊第一作者 博士研究生。研究方向：中药药效物质基础。
KEYWORDS Cyperus rotundus；habitat processing；finger-
E-mail：1543474657@qq.com
# 通信作者 教授，博士。研究方向：中药质量分析与资源。 print；content determination
E-mail：925390812@qq.com

·1706 · China Pharmacy 2022 Vol. 33 No. 14 中国药房 2022年第33卷第14期