Page 70 - 《中国药房》2022年9期

五味子配伍对补骨脂致肝细胞氧化损伤和内质网应激的影响 Δ

张婧茜 ，殷 佳 ，曲晓琳 ，刘 慧 ，王 娜 ，闫佳靓 ，傅春升 （1.山东中医药大学药学院，济南 250355；
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2.山东中医药大学附属医院乳腺甲状腺外科，济南 250014；3.山东中医药大学附属医院科技处，济南 250014）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2022）09-1088-06
DOI 10.6039/j.issn.1001-0408.2022.09.11
摘 要 目的 研究五味子配伍对补骨脂致L02肝细胞氧化损伤和内质网应激的影响，为补骨脂-五味子配伍减毒的临床应用提供
参考。方法 将 L02 细胞分为空白对照组、补骨脂模型组（1 200 μg/mL 补骨脂，以生药量计）和补骨脂-五味子低、中、高剂量组
（1 200 μg/mL补骨脂分别联用600、1 200、2 400 μg/mL五味子，以生药量计）。各组细胞加培养液或药液培养48 h后，检测细胞中
天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）水平，检测细胞培养液中谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）水
平，检测细胞中活性氧（ROS）水平及细胞线粒体膜电位，检测细胞中葡萄糖调节蛋白 78（GRP-78）、蛋白激酶 R 样内质网激酶
（PERK）mRNA及其蛋白表达水平。结果 与空白对照组比较，补骨脂模型组细胞（或其培养液）中AST、ALT、MDA、ROS水平和
GRP-78、PERK mRNA及其蛋白表达水平均显著升高（P＜0.05或P＜0.01），GSH、SOD水平和线粒体膜电位均显著降低（P＜0.05
或P＜0.01）；与补骨脂模型组比较，补骨脂-五味子低、中、高剂量组上述指标均显著改善（P＜0.05或P＜0.01）。结论 五味子与补
骨脂配伍后可以缓解补骨脂引起的L02细胞氧化损伤和内质网应激。
关键词 五味子；补骨脂；配伍减毒；肝损伤；氧化损伤；内质网应激

Effects of compatibility of Schisandra chinensis on Psoralea corylifolia-induced oxidative damage and
endoplasmic reticulum stress in hepatocytes
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ZHANG Jingxi ，YIN Jia ，QU Xiaolin ，LIU Hui ，WANG Na ，YAN Jiajing ，FU Chunsheng（1. School of
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Pharmacy，Shandong University of Traditional Chinese Medicine，Jinan 250355，China；2. Dept. of Breast and
Thyroid Surgery， the Affiliated Hospital of Shandong University of Traditional Chinese Medicine， Jinan
250014，China；3. Dept. of Science and Technology，the Affiliated Hospital of Shandong University of
Traditional Chinese Medicine，Jinan 250014，China）
ABSTRACT OBJECTIVE To study the effects of the compatibility of Schisandra chinensis on Psoralea corylifolia-induced
oxidative damage and endoplasmic reticulum stress in L02 hepatocytes， and to provide reference for clinical use of the
compatibility attenuation of P. corylifolia-S. chinensis. METHODS L02 cells were divided into blank control group，P. corylifolia
model group （1 200 μ g/mL P. corylifolia，calculated by crude drug），P. corylifolia-S. chinensis low-dose，medium-dose and
high-dose groups（1 200 μg/mL P. corylifolia combined with 600，1 200，2 400 μg/mL S. chinensis，respectively，calculated by
crude drug）. After the cells in each group were cultured in culture medium or drug solution for 48 hours，the levels of aspartate
aminotransferase（AST）and alanine aminotransferase（ALT）were detected；the levels of glutathione（GSH），superoxide dismutase
（SOD）and malondialdehyde（MDA）in cell culture medium were detected；reactive oxygen species（ROS）level and mitochondrial
membrane potential in cells were detected；mRNA and protein expressions of glucose-regulated protein 78（GRP-78）and protein
kinase R-like endoplasmic reticulum kinase（PERK）were detected. RESULTS Compared with blank control group，the levels of
AST，ALT，MDA and ROS，mRNA and protein expressions of GRP-78 and PERK were increased significantly in P. corylifolia
model group（P＜0.05 or P＜0.01）；while GSH and SOD levels，mitochondrial membrane potential were decreased significantly
（P＜0.05 or P＜0.01）. Compared with P. corylifolia model group， above indexes of P. corylifolia-S. chinensis low-dose，
medium-dose and high-dose groups were all improved significantly（P＜0.05 or P＜0.01）. CONCLUSIONS The compatibility of
P. corylifolia-S. chinensis can alleviate P. corylifolia-induced oxidative damage and endoplasmic reticulum stress of L02 cells.
KEYWORDS Schisandra chinensis；Psoralea corylifolia；compatibility attenuation；liver injury；oxidative damage；endoplasmic
reticulum stress

补骨脂为豆科植物补骨脂Psoralea coryfolia L.的干
Δ 基金项目：山东省中医药科技发展计划项目（No.2019-0079）
燥成熟果实，具有温肾助阳、纳气平喘、温脾止泻的功
＊硕士研究生。研究方向：中药制剂新剂型、新工艺。E-mail：
效，临床上常用来治疗骨质疏松症、骨软化症和皮肤
hhzhangjingxi@163.com
# 通信作者：主任药师，硕士生导师。研究方向：中药制剂新剂 病 [1－2] 。近年来有报道显示，补骨脂及其复方制剂可致
型、新工艺。E-mail：yfuchunsheng@163.com 肝损伤 [3 － 4] 。五味子为木兰科植物五味子 Schisandra

·1088 · China Pharmacy 2022 Vol. 33 No. 9 中国药房 2022年第33卷第9期