Page 82 - 《中国药房》2022年3期

诃子不同温度炮制品的化学成分含量变化及抗溃疡性结肠炎作

用比较 Δ

安悦言 ，鞠成国，张 强，郝 季，陈九妹，王 巍（辽宁中医药大学药学院，辽宁 大连 116600）
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中图分类号 R283；R285.5 文献标志码 A 文章编号 1001-0408（2022）03-0332-06
DOI 10.6039/j.issn.1001-0408.2022.03.13

摘 要 目的 研究诃子不同温度炮制品的化学成分含量变化，并比较其抗溃疡性结肠炎（UC）的效果。方法 采用砂烫法制备不
同温度（160、180、200、220、240、260、280、300 ℃）诃子炮制品。采用高效液相色谱法检测诃子生品和不同温度炮制品中没食子
酸、诃黎勒酸、诃子酸、鞣花酸的含量。将小鼠分为空白组、模型组、美沙拉嗪肠溶片组（阳性对照，0.4 g/kg）和诃子生品及不同温
度炮制品组（诃子各组给药剂量均为1.3 g/kg），每组10只。除空白组外，其余各组小鼠均从肛门一次性注入6％乙酸0.1 mL，以复
制UC模型。造模成功后，空白组和模型组小鼠灌胃水，其余各组小鼠均灌胃相应药液，给药体积均为20 mL/kg，每天1次，连续
7 d。观察各组小鼠一般体征并记录体质量，检测其结直肠长度、结直肠指数及血清中炎症相关指标[超氧化物歧化酶（SOD）、丙
二醛（MDA）、白细胞介素10（IL-10）、IL-1β、肿瘤坏死因子α（TNF-α）]的水平，观察结直肠病理形态学变化，并进行药效综合评分。
结果 随着炮制温度的升高，诃子中诃黎勒酸、诃子酸含量逐渐降低，没食子酸含量先增加再降低，鞣花酸含量逐渐增加。与模型
组比较，诃子生品和不同温度炮制品组小鼠一般体征、体质量、结直肠长度、结直肠指数及炎症相关指标水平大部分均显著改善（P＜
0.05 或 P＜0.01），结直肠组织腺体隐窝结构被修复，炎症细胞浸润减少，且在 260 ℃条件下制得的炮制品的药效综合评分最高。
结论 诃子经不同温度炮制后化学成分含量变化显著，且抗UC的效果存在差异，其中在260 ℃条件下制得的炮制品效果最优。
关键词 诃子；炮制温度；溃疡性结肠炎；没食子酸；诃黎勒酸；诃子酸；鞣花酸

Content changes of chemical constituents and comparison of the anti-ulcerative colitis effect of processed
products of Terminalia chebula at different temperatures
AN Yueyan，JU Chengguo，ZHANG Qiang，HAO Ji，CHEN Jiumei，WANG Wei（College of Pharmacy，Liaoning
University of Traditional Chinese Medicine，Liaoning Dalian 116600，China）
ABSTRACT OBJECTIVE To study the content changes of chemical constituents of processed products of Terminalia chebula at
different temperatures，and to compare its anti-ulcerative colitis effect. METHODS Processed products of T. chebula at different
temperatures（160，180，200，220，240，260，280，300 ℃）were prepared by sand scalding technology. HPLC method was
adopted to determine the contents of gallic acid，chebulagic acid，chebulinic acid and ellagic acid in crude drug and processed
products of T. chebula at different temperatures. The mice were divided into blank group，model group，Mesalazin enteric-coated
tablets group（positive control，0.4 g/kg），crude drug and processed products groups of T. chebula at different temperatures（1.3
g/kg），with 10 mice in each group. Except for blank group，other groups were given 6％ acetic acid 0.1 mL via anus to induce
ulcerative colitis model. After modeling，blank group and model group were given water intragastrically，and other groups were
given relevant drug intragastrically，20 mL/kg，once a day，for consecutive 7 days. The general physical signs of mice in each
group were observed and the body weight was recorded. The colorectal length and index，serum levels of related inflammation
indexes [superoxide dismutase （SOD），malondialdehyde （MDA），interleukin-10 （IL-10），IL-1 β ，tumor necrosis factor α
（TNF-α）] were detected. The pathomorphological changes of colon and rectum were observed，and the comprehensive score of
pharmacodynamics was performed. RESULTS With the increase of processing temperature，the contents of chebulagic acid and
chebulinic acid decreased gradually，the content of gallic acid increased first and then decreased，and the content of ellagic acid
increased. Compared with model group，the general physical signs，body weight，colorectal length，colorectal index and related
inflammation indexes were all improved significantly in crude drug and processed products groups of T. chebula at different
temperatures（P＜0.05 or P＜0.01）. The glandular recess structure of colorectal tissue was repaired，the infiltration of inflammatory
cells was reduced， and the comprehensive score of efficacy of processed products prepared at 260 ℃ was the highest.
CONCLUSIONS The contents of chemical components in T. chebula processed at different temperatures change significantly and
their anti-ulcerative colitis effects are different. The processed products of T. chebula prepared at 260 ℃ show the best
anti-ulcerative colitis effect.
Δ 基金项目：辽宁省科学技术计划项目（No.2019-ZD-0951）
KEYWORDS Terminalia chebula；processing temperature；
＊硕士研究生。研究方向：中药分析。E-mail：852762698@qq.
com ulcerative colitis； gallic acid； chebulagic acid； chebulinic
# 通信作者：副教授，硕士生导师，博士。研究方向：中药质量评 acid；ellagic acid
价及新药开发。E-mail：ww_101737@126.com

·332 · China Pharmacy 2022 Vol. 33 No. 3 中国药房 2022年第33卷第3期