Page 83 - 2020年17期

ABSTRACT OBJECTIVE：To study ongoing change characteristics of the contents of syringin and total flavonoids in different
medicinal parts（root bark，tree bark，leaf）of Toricellia angulata from Guizhou，and to provide reference for the development and
application of T. angulata. METHODS：The root bark，tree bark and leaf parts of T. angulata during different harvesting periods
（Jan.-Dec.） were taken as the research samples. The content of syringin was determined by HPLC. The determination was
performed on Agela Promosil C18 column with mobile phase consisted of 0.5％ phosphoric acid solution-acetonitrile （gradient
elution）at the flow rate of 1.0 mL/min. The detection wavelength was set as 210 nm，and column temperature was 35 ℃. The
sample size was 5 μL. The content of total flavonoids was determined by UV-visible spectrophotometry under detection wavelength
of 510 nm. RESULTS：The linear range of syringin and total flavonoids were 0.095 9-1.150 8 mg/mL（r＝0.999 6）and 0.072 2-
1.083 0 mg/mL（r＝0.999 9），respectively. RSDs of precision，stability and repeatability tests were all less than 3％（n＝6）. The
average recoveries were 101.74％ （RSD＝2.36％ ，n＝6） and 99.63％ （RSD＝2.19％ ，n＝6），respectively. During different
harvesting periods，the contents of syringin in root bark，tree bark，leaf of T. angulata collected on Aug.，May and Sept. were the
highest，and the contents of total flavonoids in samples collected on Feb.，Dec. and Sept. were the highest. The contents of syringin
in different medicinal parts of T. angulata were in descending order as follows as tree bark＞root bark＞leaf，and the content of
syringin was commonly relatively high in tree bark part；the content of total flavonoids in different medicinal parts of T. angulata
were in descending order as follows as root bark＞tree bark＞leaf，and the contents of total flavonoids in three medicinal parts was
generally low. The content of total flavonoids in root bark was the highest in Feb. of that year，and the content of syringin in root
bark at same month was second only to Aug. of that year；the content of syringin in tree bark was the highest in May，and the
content of total flavonoids in tree bark at same month was second only to Oct. and Dec. of that year；the contents of total flavonoids
and syringin in leaf were the highest in Sept. of that year. CONCLUSIONS：It is suggested that Feb. is the best time for harvesting
root bark，May for tree bark and Sept. for leaf of T. angulata.
KEYWORDS Toricellia angulata；Medicinal part；HPLC；Ongoing change；Syringin；Total flavonoids

大接骨丹药材来源于山茱萸科植物有齿鞘柄木[To- 丁香苷、总黄酮）的含量差异，评价叶和树皮部位可否作
ricellia angulata Oliv. var. intermedia（Harms）Hu]的根、 为根皮的潜在药材替代资源，并研究这2种次生代谢产
[1]
根皮、树皮及叶 ，在贵州等地方标准中以根皮入药 [2-3] 。 物的经时变化特征，以确定不同用药部位的最佳采摘时
大接骨丹中主要含有苯丙素苷类、环烯醚萜苷类、黄酮 间，为该药材的综合开发利用、民间及民族医疗机构临
苷类、二萜类等多种化学成分，已确定的单体化合物有 床应用提供参考。
[4]
紫丁香苷 、槲皮素-3-O-葡萄糖苷、黄芪苷、β-谷甾醇、硬 1 材料
脂酸、软脂酸、10-Griselinosidic acid等 [5-7] ，具有免疫调节 1.1 仪器
等作用 [8-9] 。由于齿鞘柄木多在每年的 3 月中旬前后萌 1260型高效液相色谱仪、Cary 100型紫外-可见分光
芽并在冬季落叶，故大接骨丹在贵州省少数民族聚集地 光度计（美国Agilent公司）；FA1004B型万分之一电子天
和民族医疗机构临床施药时，呈现春夏和初秋多以叶入 平（温州瑞昕仪器有限公司）；BT25S型十万分之一电子
药，而冬季和早春多以根皮入药的特点，并且有研究也 天平（德国Sartorius公司）；101-3AB型烘箱（天津市泰斯
[10]
发现大接骨丹树皮具有较好的抗炎、镇痛活性 ，提示 特仪器有限公司）；WK-1000A型高速粉碎机（潍坊市北
树皮、叶这两个部位也具有进一步开发、利用的价值。 方制药设备制造有限公司）。
目前，关于大接骨丹药材不同用药部位药效物质基 1.2 药品与试剂
础的研究相对薄弱。本课题组前期研究发现，大接骨丹 大接骨丹药材不同部位（根皮、树皮、叶）采集自贵
根皮、树皮、叶这3个部位中成分差异较大，但均存在紫 州省都匀市杉木湖沟渠，采集时间为 2017 年 1－12 月
丁香苷和总黄酮这2种次生代谢产物。且据相关文献报 （本研究开展于2018年3－6月），每个自然月的15日各
道，紫丁香苷具有抗肿瘤 、保护大鼠肝缺血再灌注损 采集药材样本 1 次（采集信息见表 1），样品经黔南民族
[11]
[12]
伤 、调节免疫、降糖、抗炎、抗氧化和抗肝毒性 等多种 医学高等专科学校药学系韩忠耀副教授鉴定分别为山
[13]
生物活性，总黄酮则具有抗氧化和抑菌 等生物活性， 茱萸科植物有齿鞘柄木[T. angulata Oliv. var. intermedia
[14]
可作为大接骨丹中指标性化学成分。此外，植物中次生 （Harms）Hu]的根皮、树皮和叶；紫丁香苷对照品（北京
代谢产物的动态积累规律可用于中药材、民族药品质优 盛世康普化工技术研究院，批号：161214，纯度：≥
劣的评价 。鉴于此，本研究以紫丁香苷和总黄酮含量 98％）；芦丁对照品（成都曼思特生物科技有限公司，批
[15]
作为质量控制与药材品质评价指标，考察大接骨丹药材 号：MUST-17122001，纯度：≥98％）；乙腈（天津科密欧
3 个用药部位（根皮、树皮、叶）中 2 种次生代谢产物（紫 化学试剂有限公司，色谱纯）；其余试剂均为分析纯，水

中国药房 2020年第31卷第17期 China Pharmacy 2020 Vol. 31 No. 17 ·2125 ·