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老鼠簕生物碱A对大鼠非酒精性脂肪肝的改善作用及其机制研

究 Δ

徐万鹏 ，梁英琴，韦秀桂，王红园，张 华，周焕芳，林 兴，林 军（广西医科大学药学院，南宁 530021）
#
＊
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2020）16-1955-06
DOI 10.6039/j.issn.1001-0408.2020.16.07

摘 要 目的：研究老鼠簕生物碱A（HBOA）对大鼠非酒精性脂肪肝的改善作用及其机制。方法：将SD大鼠随机分为空白对照
组、模型组、水飞蓟宾胶囊组（阳性对照，26.25 mg/kg）和HBOA高、中、低剂量组（100、50、25 mg/kg），每组10只。除空白对照组喂
养普通饲料外，其余各组大鼠连续喂养高脂饲料8周以复制非酒精性脂肪肝模型。于第9周起，空白对照组和模型组大鼠灌胃等
体积0.6％羟甲基纤维素钠溶液，各给药组大鼠灌胃相应药物，每日1次，连续4周。观察各组大鼠一般情况并计算其体质量增量、
脏器（肝、肾、脾）指数；检测肝组织中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、总胆固醇（TC）、三酰甘油（TG）、游离脂肪酸
（NEFA）含量，以及血清中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛（MDA）含量；采用免疫组织化学
法检测肝组织中过氧化物酶体增殖物激活受体α（PPARα）蛋白的表达情况。结果：与空白对照组比较，模型组大鼠体质量增量和
肝指数均显著升高（P＜0.01）；肝内可见脂肪沉积现象，血清中 SOD、GSH-Px 活性均显著降低，MDA 含量以及肝组织中 AST、
ALT、TC、TG、NEFA含量均显著升高，PPARα蛋白表达水平显著降低（P＜0.01）。与模型组比较，各给药组大鼠体质量增加量和肝
指数均显著降低（P＜0.05 或 P＜0.01），肝内脂肪沉积现象改善，血清中 SOD、GSH-Px 活性（除 HBOA 低剂量组外）均显著升高，
MDA含量以及肝组织中AST、ALT、TC（除HBOA低剂量组外）、TG（除HBOA低剂量组外）、NEFA含量均显著降低，PPARα蛋白
表达水平（除 HBOA 低剂量组外）均显著升高（P＜0.05 或 P＜0.01），且高剂量组上述部分指标显著优于中、低剂量组（P＜0.05）。
结论：老鼠簕生物碱A对高脂饮食致大鼠非酒精性脂肪肝有一定的改善作用，其机制可能与改善脂质代谢紊乱、抗氧化应激及上
调PPARα表达有关。
关键词 老鼠簕生物碱A；大鼠；非酒精性脂肪肝；氧化应激；脂代谢紊乱

Study on the Improvement Effect and Mechanism of Ilicifoliosids Alkaloid A on Non-alcoholic Fatty Liver
Disease in Rats
XU Wanpeng，LIANG Yingqin，WEI Xiugui，WANG Hongyuan，ZHANG Hua，ZHOU Huanfang，LIN Xing，LIN
Jun（College of Pharmacy，Guangxi Medical University，Nanning 530021，China）

ABSTRACT OBJECTIVE：To study improvement effect and mechanism of ilicifoliosids alkaloid A（HBOA）on non-alcoholic
fatty liver disease in rats. METHODS：SD rats were randomly divided into blank control group，model group，Silybin capsule
group（positive control，26.25 mg/kg），HBOA high-dose，medium-dose and low-dose groups（100，50，25 mg/kg），with 10 rats
in each group. Except that blank control group fed normal feed，the other groups were continuously fed with high-fat diet for 8
weeks to induce non-alcoholic fatty liver disease model. Form the 9th week，blank control group and model group were given
constant volume of 0.6％ CMC-Na solution，and administration groups were given corresponding drugs by intragastric admini-
stration，once a day，for consecutive 4 weeks. The general information of rats were observed and the body weight increase，organ
（liver，kidney and spleen）indexes were calculated；the contents of AST，ALT，TC，TG and NEFA in liver tissue were detected，
and SOD，GSH-Px activities and MDA content in the serum were also determined. The protein expression of PPARα in liver tissue
was detected by immunohistochemistry. RESULTS：Compared with blank control group，the body mass increase and liver index of
rats in model group were increased significantly（P＜0.01）；fat deposition could be observed in the liver；the activities of SOD and
GSH-Px in serum were reduced significantly，and the contents of MDA，the contents of AST，ALT，TC，TG and NEFA in liver
tissue were significantly increased，and the protein expression of PPARα was decreased significantly（P＜0.01）. Compared with
model group，the body mass increase and liver index of the rats were decreased significantly in administration groups（P＜0.05 or
P＜0.01），liver fat deposition was improved，the activity of SOD and GSH-Px in serum（except for HBOA low-dose group）were
increased significantly while MDA content，the contents of AST，ALT，TC（except for HBOA low-dose group），TG（except for
HBOA low-dose group） and NEFA in liver tissue were
Δ 基金项目：国家自然科学基金资助项目（No.81660106）
decreased significantly，while protein expression of PPAR α
＊ 药 师 ，硕 士 研 究 生 。 研 究 方 向 ：中 药 药 理 学 。 E-mail：
15177460685@163.com was increased significantly （P＜0.05 or P＜0.01）. Some of
# 通信作者：教授，硕士生导师，博士。研究方向：中药药理学。 the above indexes of HBOA high-dose group were
电话：0771-5302433。E-mail：junlin898@126.com significantly better than HBOA medium- and low-dose group

中国药房 2020年第31卷第16期 China Pharmacy 2020 Vol. 31 No. 16 ·1955 ·