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HPLC指纹图谱结合正交试验优化红参的炮制工艺 Δ

侯新莲 ，黄 露 ，彭 成 ，朱雅宁 ，蔡帮军 ，周勤梅 2，3 # [1.华润三九（雅安）药业有限公司，四川 雅安
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625000；2.成都中医药大学药学院，成都 611137；3.西南特色药材创新药物成分研究所，成都611137]
中图分类号 R284.1 文献标志码 A 文章编号 1001-0408（2020）10-1228-06
DOI 10.6039/j.issn.1001-0408.2020.10.14

摘 要 目的：建立红参的高效液相色谱（HPLC）指纹图谱，并优选其最优炮制工艺。方法：采用 HPLC 法。色谱柱为 Waters
SymmetryShield TM RP18，流动相为乙腈-水（梯度洗脱），柱温为30 ℃，流速为1.0 mL/min，检测波长为203 nm，进样量为10 μL。以
人参皂苷Rb1为参照，绘制10批红参样品的HPLC指纹图谱；采用《中药色谱指纹图谱相似度评价系统（2012A版）》进行相似度评
价，并确定共有峰。以蒸制温度、蒸制时间、干燥方法为考察因素，人皂苷类成分含量、指纹图谱相似度为指标，采用L16 （4）正交试
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验优化红参的炮制工艺并进行验证；采用SPSS 19.0软件对10批红参样品和最优工艺炮制品进行聚类分析。结果：10批红参共有
13 个共有峰，相似度均大于 0.920；共指认人参皂苷 Rg1、人参皂苷 Re、人参皂苷 Rb1等 3 个共有峰。最优炮制工艺为 100 ℃蒸制
150 min，60 ℃干燥；验证试验结果显示，3批红参最优工艺炮制品中人参皂苷Rg1、Re、Rb1的含量分别为0.26％～0.29％、0.17％～
0.20％、0.47％～0.54％，其指纹图谱与对照图谱相似度均大于0.970。聚类分析结果显示，10批红参及3批最优工艺炮制品可聚为
两类，即HS3～HS10聚为一类、3批最优工艺炮制品及HS1、HS2聚为一类。结论：所建指纹图谱可用于红参的炮制工艺优化，能
表征炮制工艺参数波动与药材整体质量的相关性变化；所得最优炮制工艺合理可行。
关键词 红参；炮制工艺优化；正交试验；高效液相色谱法；指纹图谱；聚类分析

Optimization of Processing Technology of Ginseng Radix et Rhizoma Rubra by HPLC Fingerprint
Combined with Orthogonal Test
HOU Xinlian ，HUANG Lu ，PENG Cheng ，ZHU Yaning ，CAI Bangjun ，ZHOU Qinmei 2，3 [1. China Resources
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Sanjiu（Ya’an） Pharmaceutical Co.，Ltd.，Sichuan Ya’an 625000，China；2. Pharmacy College，Chengdu
University of TCM，Chengdu 611137，China；3. Institute of Innovative Medicine Ingredients of Southwest
Specialty Medicinal Materials，Chengdu 611137，China]
ABSTRACT OBJECTIVE：To establish an HPLC fingerprint of Ginseng Radix et Rhizoma Rubra，and to optimize its processing
technology. METHODS：HPLC method was adopted. The determination was performed on Waters SymmetryShield TM RP18 column
with mobile phase consisted of acetonitrile-water（gradient elution）at the flow rate of 1.0 mL/min. The column temperature was set
at 30 ℃，and the detection wavelength was 203 nm. The sample size was 10 μL. Using ginsenoside Rb1 as reference peak，HPLC
fingerprints of 10 batches of Ginseng Radix et Rhizoma Rubra was established. The similarity of them was evaluated by using
Similarity Evaluation System of TCM Chromatogram Fingerprint （2012 A edition） to confirm common peak. With steaming
temperature，time and drying method as factors，using the content of ginsenoside and fingerprint similarity as index，the processing
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technology was optimized with L16 （4）orthogonal test design and verified. Cluster analysis was conducted with SPSS 19.0 statistical
software of 10 batches of Ginseng Radix et Rhizoma Rubra and 3 batches of optimal processed sample. RESULTS：There were a
total of 13 common peaks in the fingerprints of 10 batches of Ginseng Radix et Rhizoma Rubra. The similarity was more than
0.920；3 common peaks were identified，such as ginsenoside Rg1，ginsenoside Re，ginsenoside Rb1. The optimal processing
technology included that steamed at 100 ℃ for 150 min，dried at 60 ℃. The results of validation test show that the contents of
ginsenoside Rg1，Re and Rb1 were 0.26％-0.29％，0.17％-0.20％，0.47％-0.54％，and the similarity between 3 batches of Ginseng
Radix et Rhizome Rubra optimal processed sample and the control fingerprints was more than 0.970. The results of cluster analysis
showed that 10 batches of Gimseng Radix et Rhizoma Rubra and 3 batches of optimal processed sample could be clustered into two
categories；HS3-HS10 could be clustered into one category，and 3 batches of optimal processed sample，HS1 and HS2 be clustered
into one category. CONCLUSIONS：Established fingerprint can be used for the optimization of processing technology of Gimseng
Radix et Rhizoma Rubra，and characterize the correlation between fluctuation of technology parameter and quality of medicinal
material；the optimal processing technology is reasonable and
Δ 基金项目：国家中药标准化项目（No.ZYBZH-C-SC-51）
feasible.
＊工程师。研究方向：中成药工艺、质量标准。电话：0835-
KEYWORDS Ginseng Radix et Rhizoma Rubra；Processing
2876059。E-mail：357534794@qq.com
technology optimization； Orthogonal test design； HPLC；
# 通信作者：实验师，博士。研究方向：中药药效物质基础、质量
标准。电话：028-61800087。E-mail：793233279@qq.com Fingerprint；Cluster analysis

·1228 · China Pharmacy 2020 Vol. 31 No. 10 中国药房 2020年第31卷第10期