Page 62 - 2020年1月第31卷第2期

Fingerprint Establishment， Content Determination and α-glucosidase Inhibitory Activity Study of
Polysaccharide from Desmodium styracifolium
CHENG Xuanxuan，CHEN Liangyuan，ZHENG Shijia，TANG Xiaomin，YANG Quan（School of Traditional
Chinese Medicine，Guangdong Pharmaceutical University/Key Laboratory of State Administration of Traditional
Chinese Medicine for Production & Development of Cantonese Medicinal Materials/Guangzhou Comprehensive
Experimental Station of National Industrial Technology System for Chinese Materia Medica/Guangdong
Engineering Research Center of Good Agricultural Practice & Comprehensive Development for Cantonese
Medicinal Materials，Guangzhou 510006，China）

ABSTRACT OBJECTIVE：To establish the fingerprint，analyze the monosaccharide composition and content，investigate the
inhibitory effects of the polysaccharide from Desmodium styracifolium on α-glucosidase in vitro. METHODS：Polysaccharide from
D. styracifolium was prepared by water extraction and ethanol precipitation. After hydrolyzed by TFA and derived by PMP，HPLC
method was adopted to establish the fingerprint （using glucose peak as reference），and analyze the constituent and content of
monosaccharide. The content determination was performed on Phenomenex Luna C18 column with mobile phase consisted of
acetonitrile-0.05 mol/L potassium phosphate（pH adjusted to 6.8 with sodium hydroxide）in gradient elution at the flow rate of 0.8
mL/min. The detection wavelength was set at 250 nm，and column temperature was set at 30 ℃. The sample size was 10 μL. Using
acarbose as control， PNPG assay was used to investigate the α-glucosidase inhibitory activity of polysaccharide from D.
styracifolium. RESULTS：There were 9 common peaks in HPLC fingerprints of 18 batches of samples，and the similarity of 15
batches of samples was higher than 0.90. Totally 7 peaks were identified as mannose，rhamnose，galacturonic acid，glucose，
galactose，xylose and arabinose. The contents of rhamnose，galacturonic acid，glucose，galactose and arabinose were 0.471-2.092，
1.379-8.919，2.560-35.679，1.194-6.905，0.566-4.158 mg/g，respectively. Based on rhamnose，the molar ratios of the other four
monosaccharides were 1.58-4.07，2.26-19.95，2.20-4.21 and 1.31-2.86，respectively. The inhibitory activity of polysaccharide from
D. styracifolium on α-glucosidase increased with the increase of dose，and the half inhibitory concentrations of it was 0.70 mg/mL，
lower than 7.76 mg/mL of acarbose （positive control）. CONCLUSIONS：Glucose is the main component of D. styracifolium
polysaccharide in different batches，and the contents of monosaccharides are different. The polysaccharide from D. styracifolium
have significant inhibitory activity on α-glucosidase，which is better than that of acarbose.
KEYWORDS Polysaccharide from Desmodium styracifolium；Monosaccharide；Content；Fingerprint；HPLC；α-glucosidase；
Inhibitory activity

广金钱草为豆科植物广金钱草[Desmodium styraci- 列（DAD）检测器、SIL-20A 型自动进样器、CTO-20A 型
folium（Osb.）Merr.]的干燥地上部分，味甘、淡，性凉，归 柱温箱、DGU-20A3 型真空脱气机、LC-20AT 型溶液传
肝、肾、膀胱经，具有利湿退黄、利尿通淋之效，主治黄疸 输单元（日本 Shimadzu 公司）；BP211D 型十万分之一电
[1]
尿赤、热淋、石淋、小便涩痛等症 。广金钱草中的黄酮 子天平（德国Sartorius公司）；TG-16WS型台式高速离心
类成分因具有利尿、抗氧化、抑制大鼠肾结石形成、改善 机（长沙湘智离心机仪器有限公司）；UV-5200PC 型紫
大鼠离体心脏缺血再灌注损伤等广泛的药理活性，是评 外-可见分光光度计（上海元析仪器有限公司）；Multi-
价药材质量的指标性成分，备受学者关注 [2-5] 。除黄酮类 skan GO 型酶标仪（美国 Thermo Fisher Scientific 公司）；
成分外，多糖在广金钱草中的含量也较高，但其相关药 Biosafer-20TB型实验室级超纯水器[赛飞（中国）有限公
[6]
[7]
理学研究较少。考虑到植物多糖在抗肿瘤 、抗衰老 、 司]；XMTD-8222 型电热恒温水浴锅、PH27-9240A 型电
[8]
[9]
免疫调节 、降血脂 等方面独特的生物活性，开发前景 热恒温鼓风干燥箱（上海精宏实验设备有限公司）；
良好，故本研究拟从广金钱草药材中提取多糖，采用 1- ST2100 型实验室 pH 计[奥豪斯仪器（常州）有限公司]；
苯基-3-甲基-5-吡唑啉酮（PMP）柱前衍生化-高效液相色 N-1100D-WD型旋转蒸发仪（日本Eyela公司）。
谱法（HPLC）分析其单糖组成，并建立不同产地广金钱 1.2 药品与试剂
草多糖的指纹图谱；在测定多糖含量的基础上，初步评 葡萄糖对照品（批号：AF7022401）、阿拉伯糖对照品
价其对α-葡萄糖苷酶的抑制作用，旨在为全面开发利用 （批号：AF8051683）均购自成都埃法生物科技有限公司；
该药材资源提供依据。 甘露糖对照品（批号：40708）、半乳糖对照品（批号：
1 材料 10509）、鼠李糖对照品（批号：30521）均购自德国Dr. Eh-
1.1 仪器 renstorfer GmbH 公司；半乳糖醛酸对照品（上海金穗生
LC-20AT 型 HPLC 仪，配有 SPD-M20A 型二极管阵 物科技有限公司，批号：20180422）；木糖对照品（上海麦

·184 · China Pharmacy 2020 Vol. 31 No. 2 中国药房 2020年第31卷第2期