Page 66 - 2019年9月第30卷第17期

门氏护胃方中连翘与其他药材配伍煎煮前后连翘酯苷A和连翘

苷提取率变化的研究 Δ

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梁惠珍 ，靳贝贝 ，裴香萍 ，门九章 ，李 慧 ，魏金娜（1.山西中医药大学中药学院，山西 晋中 030619；2.山
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西中医药大学中西医结合临床学院，山西 晋中 030619；3.黑龙江中医药大学中药学院，哈尔滨 150040；4.天
津中医药大学中药学院，天津 301617）
中图分类号 R927.2；R932 文献标志码 A 文章编号 1001-0408（2019）17-2365-05
DOI 10.6039/j.issn.1001-0408.2019.17.12

摘 要 目的：考察门氏护胃方中连翘单药及与其他药材配伍煎煮前后连翘酯苷A和连翘苷提取率的变化。方法：采用高效液相
色谱法测定连翘单味药材（5 g×7付）、连翘（5 g×7付）与姜半夏配伍、门氏护胃方[包括连翘（5 g×7付）、姜半夏等6味药材]经水煎
煮后药液中连翘酯苷A和连翘苷的提取量并计算提取率。色谱柱为 Diamonsil C18，流动相为乙腈-0.2％甲酸溶液，梯度洗脱，检测
波长为 278 nm，柱温为 30 ℃，流速为 1.0 mL/min。结果：连翘酯苷 A 与连翘苷进样量线性范围分别为 0.61～6.1、0.246～2.46 μg
（r＝0.999 7、0.999 9），在精密度、稳定性（20 h 内）和重复性试验中 RSD 均＜2％（n＝6），平均回收率为 96.10％～99.37％（RSD≤
2.36％，n＝6）；在连翘单味药材中，连翘酯苷A和连翘苷的提取率分别为96.90％、66.67％；在连翘-姜半夏配伍样品水煎液中，二
者的提取率分别为101.61％、54.55％；在门氏护胃方样品水煎液中，二者的提取率分别为98.39％、84.85％。结论：连翘与姜半夏
配伍后连翘酯苷A的提取率增加、连翘苷的提取率略有减少；连翘与门氏护胃方中其他药材配伍后，二者的提取率均略有增加。
关键词 门氏护胃方；连翘酯苷A；连翘苷；配伍；含量测定；高效液相色谱法

Study on Changes of Extraction Rates of Forsythiaside A and Forsythin in Forsythia suspense Compatible
with Other Medicinal Materials of Menshi Huwei Formula before and after Decoction
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LIANG Huizhen ，JIN Beibei ，PEI Xiangping ，MEN Jiuzhang ，LI Hui ，WEI Jinna（1.College of TCM，Shanxi
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University of TCM，Shanxi Jinzhong 030619，China；2.Clinical College of Integrated Traditional Chinese and
Western Medicine， Shanxi University of TCM， Shanxi Jinzhong 030619， China；3.College of TCM，
Heilongjiang University of TCM，Harbin 150040，China；4.College of TCM，Tianjin University of TCM，
Tianjin 301617，China）
ABSTRACT OBJECTIVE：To investigate the changes of extraction rates of forsythiaside A and forsythin in Forsythia suspensa
compatible with other medicinal material of Menshi huwei formula before and after decoction. METHODS：HPLC method was
used to determine the extraction amounts and to calculate the extraction rates of forsythiaside A and forsythin in F. suspensa（5 g×7
doses），F. suspensa（5 g×7 doses）compatible with Pinelliae rhizoma praeparatum cum zingibere et alumine（PRZA），Menshi
huwei formula [including 6 ingredients as F. suspense（5 g×7 doses），PRZA] after decocted with water. The determination was
performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-0.2％ formic acid solution（gradient elution）. The
detection wavelength was set at 278 nm，and the column temperature was 30 ℃. The flow rate was 1.0 mL/min. RESULTS：The
linear range of forsythiaside A and forsythin were 0.61-6.1，0.246-2.46 μ g （r＝0.999 7，0.999 9），respectively；RSDs of
precision， stability （within 20 h） and reproducibility tests were all lower than 2％ （n＝6）. Average recovery rates were
96.10％-99.37％（RSD≤2.36％，n＝6）respectively. In F. suspensa，extraction rates of forsythiaside A and forsythin were 96.90％
and 66.67％ . In F. suspensa compatible with PRZA，extraction rates of them were 101.61％ and 54.55％ . In Menshi huwei
formula，extraction rates of them were 98.39％ and 84.85％. CONCLUSIONS：After F. suspensa is compatible with PRZA，the
extraction rates of forsythiaside A is increased while forsythin is decreased. After compatible with other medicinal material in
Menshi huwei formula，extraction rates of both are increased slightly.
KEYWORDS Menshi huwei formula；Forsythiaside A；Forsythin；Compatibility；Content determination；HPLC
Δ 基金项目：山西省重点研发计划重点项目（No.201603D3113021）；
晋药综合开发利用协同创新中心项目（No.2017-JYXT-05）
门氏护胃方是山西首届名医门九章教授的经验方，
＊副教授，硕士。研究方向：中药复方药效物质基础。E-mail：
由人参、连翘、炙甘草、姜半夏等药味组成，具有温中止
2191767170@qq.com
# 通信作者：副教授，硕士生导师，硕士。研究方向：中药质量控 呕、补脾散寒的功效，用于治疗癌症患者，特别是呕吐严
制及药效物质基础。E-mail：peixp69@163.com 重的癌症患者放化疗后的胃肠道反应。方中连翘和姜

中国药房 2019年第30卷第17期 China Pharmacy 2019 Vol. 30 No. 17 ·2365 ·