Page 56 - 《中国药房》2025年10期

泽泻汤传统汤剂和配方颗粒降低脂质堆积的作用机制和药效研究 Δ

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郭媛媛 ，马丽娜 ，蔺虎琴 ，郑长辉 ，李佳怡 ，李旨君 ，曹俊岭 （1. 北京中医药大学中药学院，北京
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100029；2. 北京中医药大学东方医院药学部，北京 100078；3. 北京中医药大学东直门医院药学部，北京
100700；4.北京中医药大学东直门医院洛阳医院药学部，河南 洛阳 471934）
中图分类号 R965；R285.5 文献标志码 A 文章编号 1001-0408（2025）10-1202-07
DOI 10.6039/j.issn.1001-0408.2025.10.09
摘 要 目的 通过网络药理学探究泽泻汤降低脂质堆积的作用机制，并比较泽泻汤传统汤剂、配方颗粒降低脂质堆积的药效差
异。方法 采用TCMSP、SwissTargetPrediction数据库检索泽泻汤活性成分和靶点；采用GeneCards、OMIM、DisGeNET、TTD数据
库分析非酒精性脂肪性肝病（NAFLD）相关靶点；采用 String 数据库构建交集靶点蛋白质-蛋白质相互作用网络模型；运用 Cyto‐
Scape 软件构建“泽泻汤-NAFLD靶点-通路”网络图；利用Metascape平台进行靶点富集分析。建立人肝癌HepG2细胞脂质堆积模
型，以吸光度值反映泽泻汤传统汤剂、配方颗粒对细胞脂质堆积的影响。结果 泽泻汤治疗NAFLD的关键活性成分包括泽泻醇
B、泽泻醇C、一亚油酸甘油酯、泽泻醇B单乙酸酯等，核心靶点包括MDM2、MAPK1、PIK3CB、PRKCQ、MAPK14等，核心信号通
路包括内分泌抵抗、胰岛素抵抗、辅助性T细胞17细胞分化等。与模型组比，除泽泻配方颗粒组、白术配方颗粒组外，各给药组细
胞吸光度值均显著降低（P＜0.01）；白术传统汤剂组吸光度值显著高于泽泻汤传统汤剂组（P＜0.01）；泽泻配方颗粒组、白术配方
颗粒组吸光度值均显著高于泽泻汤配方颗粒组（P＜0.01）；泽泻配方颗粒组吸光度值显著高于泽泻传统汤剂组（P＜0.01）；白术配
方颗粒组吸光度值显著高于白术传统汤剂组（P＜0.01）。结论 泽泻汤通过多成分、多靶点、多通路降低人肝癌细胞脂质堆积，其
传统汤剂与配方颗粒降脂效果略有差异。
关键词 泽泻汤；传统汤剂；配方颗粒；非酒精性脂肪性肝病；人肝癌细胞；脂质堆积

Comparative study on the mechanism and efficacy of Zexie tang traditional decoction and formula
granules in reducing lipid accumulation
GUO Yuanyuan ，MA Lina ，LIN Huqin ，ZHENG Changhui ，LI Jiayi ，LI Zhijun ，CAO Junling （1. School of
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Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100029， China；2. Dept. of
Pharmacy， Dongfang Hospital， Beijing University of Chinese Medicine， Beijing 100078， China；3. Dept. of
Pharmacy， Dongzhimen Hospital， Beijing University of Chinese Medicine， Beijing 100700， China；4. Dept. of
Pharmacy， Luoyang Hospital， Dongzhimen Hospital， Beijing University of Chinese Medicine， Henan Luoyang
471934， China）
ABSTRACT OBJECTIVE To explore the effect and mechanism of Zexie tang （ZXT） on reducing lipid accumulation through
network pharmacology， and compare the difference of traditional decoction versus formula granules. METHODS The active
components and targets of ZXT were identified using TCMSP and SwissTargetPrediction databases. GeneCards， OMIM， DisGeNET
and TTD databases were used to analyze the related targets of non-alcoholic fatty liver disease （NAFLD）； protein-protein
interaction network model was constructed by String database； “ZXT-NAFLD target-pathway” network diagram was constructed by
using CytoScape software； target enrichment analysis was performed by using Metascape platform. Fat accumulation model of
human hepatocellular carcinoma HepG2 cells was established to observe the effects of traditional decoction and formula granules of
ZXT on lipid accumulation of cells. RESULTS Alisol B， alisol C， 1-monolinolein and alisol B monoacetate were the key active
components of ZXT in the treatment of NAFLD. The core targets included MDM2， MAPK1， PIK3CB， PRKCQ and MAPK14，
etc. The core signaling pathways included endocrine resistance， insulin resistance and Th17 cell differentiation. Compared with
model group， except for the Zexie formula granules group and Baizhu formula granules group， the absorbance values in all other
administration groups were significantly decreased （P＜0.01）； the absorbance value of Baizhu traditional decoction group was
significantly higher than that of ZXT traditional decoction
Δ 基金项目 河南省中医药科学研究专项课题（No.2023ZYZD14）
＊第一作者 博士研究生。研究方向：中药临床合理使用。E- group （P＜0.01）； the absorbance values of Zexie formula
mail：734743865@qq.com granule group and Baizhu formula granule group were
# 通信作者 主任药师，博士生导师，博士。研究方向：医院药学及 significantly higher than that of ZXT formula granule group
合理用药。E-mail：caojunling72@163.com （P＜0.01）； the absorbance value of Zexie formula granule

· 1202 · China Pharmacy 2025 Vol. 36 No. 10 中国药房 2025年第36卷第10期