Page 20 - 《中国药房》2024年21期

竹沥半夏炮制前后HPLC指纹图谱及4种核苷类成分含量比较
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郑琳玉 1， 2＊ ，朱伟豪 ，罗美美 ，梅春梅 ，李伟东 ，许 垒 ，黄玉宇 （1. 南京中医药大学药学院，南京
1， 2
210023；2.江苏省中药炮制重点实验室，南京 210023；3.苏州聚鑫堂药业有限公司，江苏 常熟 215500；
4.南京中医药大学附属苏州市中医医院药学部，江苏 苏州 215009）
中图分类号 R284 文献标志码 A 文章编号 1001-0408（2024）21-2590-06
DOI 10.6039/j.issn.1001-0408.2024.21.03

摘 要 目的 比较姜半夏与其竹沥炮制品的高效液相色谱（HPLC）指纹图谱及核苷类成分变化，为竹沥半夏的质量评价提供参
考。方法 采用《中药色谱指纹图谱相似度评价系统（2012版）》建立10批姜半夏及其炮制品竹沥半夏的HPLC指纹图谱，并对其
共有峰进行系统聚类分析（HCA）、主成分分析（PCA）和正交偏最小二乘-判别分析（OPLS-DA）；测定姜半夏与竹沥半夏中次黄嘌
呤、尿苷、腺嘌呤、鸟苷共4种核苷类成分的含量。结果 10批姜半夏和竹沥半夏与其对应对照指纹图谱R的相似度均在0.851～
0.990之间。2种样品共得到10个共有峰，并指认出了4个成分，分别为次黄嘌呤、尿苷、腺嘌呤、鸟苷。HCA、PCA、OPLS-DA结果
均显示，姜半夏、竹沥半夏样品分别聚为一类，且OPLS-DA筛选出姜半夏与竹沥半夏4个差异性成分，根据变量重要性投影值排
序依次为峰8、峰1、峰6（腺嘌呤）、峰10。含量测定结果显示，竹沥半夏中次黄嘌呤、尿苷、腺嘌呤、鸟苷的平均含量较姜半夏分别
下降了15.90%、12.00%、26.04%、22.18%，其中次黄嘌呤、腺嘌呤、鸟苷含量差异具有统计学意义（P＜0.05或P＜0.01）。结论 本研
究建立的指纹图谱及含量测定方法操作简便、重复性好，可用于姜半夏和竹沥半夏的定性、定量分析；竹沥炮制姜半夏后4种核苷
类成分的平均含量降低。
关键词 竹沥半夏；姜半夏；指纹图谱；含量测定；核苷类成分；质量控制

Comparison of HPLC fingerprint and contents of four nucleoside components before and after processing
of Succus bambusae pinella preparata
ZHENG Linyu ，ZHU Weihao ，LUO Meimei ，MEI Chunmei ，LI Weidong ，XU Lei ，HUANG Yuyu 4
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（1. School of Pharmacy， Nanjing University of Chinese Medicine， Nanjing 210023， China；2. Jiangsu Key
Laboratory of Chinese Medicine Processing， Nanjing 210023， China；3. Suzhou Juxintang Pharmaceutical Co.，
Ltd.， Jiangsu Changshu 215500， China. 4. Department of Pharmacy， Suzhou TCM Hospital Affiliated to
Nanjing University of Chinese Medicine， Jiangsu Suzhou 215009， China）
ABSTRACT OBJECTIVE To investigate the changes in high performance liquid chromatography （HPLC） fingerprint spectra
and nucleoside components between Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine and its processed product Succus
bambusae pinella preparata， providing a reference for the quality evaluation of the latter. METHODS HPLC fingerprint was
established for 10 batches of Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine and its processed product Succus bambusae
pinella preparata following the Similarity Evaluation System of TCM Chromatographic Fingerprints （2012 Edition）. Hierarchical
cluster analysis （HCA）， principal component analysis （PCA）， and orthogonal partial least squares-discriminant analysis （OPLS-
DA） were conducted on their common peaks. The contents of four nucleoside components， hypoxanthine， uridine， adenine， and
guanosine， in both Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine and Succus bambusae pinella preparata were
determined. RESULTS The similarity between the fingerprints of the 10 batches of Pinelliae Rhizoma Praeparatum cum Zingibere
et Alumine， Succus bambusae pinella preparata， and their corresponding reference fingerprints ranged from 0.851 to 0.990. A total
of 10 common peaks were obtained for both samples， and 4 components were identified as hypoxanthine， uridine， adenine， and
guanosine. The results of HCA， PCA and OPLS-DA showed that the samples of Pinelliae Rhizoma Praeparatum cum Zingibere et
Alumine and Succus bambusae pinella preparata were clustered into separate categories， with OPLS-DA selecting 4 differential
components between them， ranked by variable importance projection values as peak 8， peak 1， peak 6 （adenine） and peak 10. The
content determination results showed that the average contents of hypoxanthine， uridine， adenine and guanosine in Succus
bambusae pinella preparata declined by 15.90%， 12.00%， 26.04% and 22.18% compared to Pinelliae Rhizoma Praeparatum cum
Zingibere et Alumine， respectively， with statistically
Δ 基金项目 2022年中央财政转移支付地方项目 significant differences in the contents of hypoxanthine， adenine
＊第一作者 硕士研究生。研究方向：中药制药技术与产品研发。
and guanosine （P＜0.05 or P＜0.01）. CONCLUSIONS The
E-mail：1205778367@qq.com
# 通信作者 主任中药师，硕士生导师。研究方向：医院药学。 established fingerprint and content determination methods are
E-mail：Yuyuhuang-sz@163.com simple to operate and have good repeatability， which are

· 2590 · China Pharmacy 2024 Vol. 35 No. 21 中国药房 2024年第35卷第21期