Page 48 - 《中国药房》2024年10期
P. 48
基于指纹图谱与化学计量学的桑叶蜜炙前后差异性标志物研究
Δ
1＊
2
3 #
2
1
2
闫蓓蓓 ，张晓平 ，秦焕云 ，赵祥君 ，邵冰梅 ，田景振 ，马大龙 （1.山东中医药大学药学院，济南 250355；
2
2. 山东中医药大学青岛中医药科学院，山东 青岛 266000；3. 青岛大学附属医院药品调剂科，山东 青岛
266005）
中图分类号 R284；R917 文献标志码 A 文章编号 1001-0408（2024）10-1198-05
DOI 10.6039/j.issn.1001-0408.2024.10.08
摘 要 目的 比较桑叶蜜炙前后化学成分的变化，筛选差异性标志物并进行含量测定，为蜜炙桑叶的质量控制提供参考。方法
采用高效液相色谱（HPLC）法建立桑叶蜜炙前后指纹图谱，标定共有峰并进行相似度评价；以生桑叶、蜜炙桑叶共有峰峰面积为指
标，运用主成分分析和正交偏最小二乘-判别分析筛选桑叶蜜炙前后差异性标志物，并进行定量分析。结果 从10批生桑叶、10批
蜜炙桑叶HPLC指纹图谱中分别标定出23、24个共有峰，2类样品图谱的相似度均大于0.97；主成分分析结果显示，生桑叶和蜜炙
桑叶分为2类，正交偏最小二乘-判别分析结果显示，峰2、峰H（5-羟甲基糠醛）、峰1、峰17（异绿原酸C）、峰16的变量重要性投影
值均大于1；生桑叶、蜜炙桑叶中差异性标志物异绿原酸C的平均含量分别为0.093 6、0.127 8 mg/g，差异有统计学意义（P＜0.05）。
结论 筛选得到异绿原酸C等5种差异性标志物；桑叶蜜炙后异绿原酸C含量显著升高。
关键词 桑叶；蜜炙桑叶；指纹图谱；化学计量学；差异性标志物
Study on the differential markers of Morus alba leaves before and after baked with honey based on
fingerprint and chemometrics
1
2
2
2
YAN Beibei ，ZHANG Xiaoping ，QIN Huanyun ，ZHAO Xiangjun ，SHAO Bingmei ，TIAN Jingzhen ，MA
1
2
Dalong（1. School of Pharmacy， Shandong University of Traditional Chinese Medicine， Jinan 250355， China；
3
2. Qingdao Academy of Chinese Medical Sciences， Shandong University of Traditional Chinese Medicine， Shandong
Qingdao 266000， China；3. Dept. of Drug Dispensing， the Affiliated Hospital of Qingdao University， Shandong
Qingdao 266005， China）
ABSTRACT OBJECTIVE To compare the changes of chemical components of Morus alba leaves， screen differential markers，
and determine their contents， so as to provide reference for quality control of M. alba leaves before and after baked with honey.
METHODS The fingerprints of M. alba leaves before and after baked with honey were established by high-performance liquid
chromatography （HPLC）. The common peaks of the fingerprints were identified and the similarity was evaluated. The differential
markers of M. alba leaves before and after baked with honey were screened by principal component analysis （PCA） and orthogonal
partial least squares-discriminant analysis （OPLS-DA） using common peak are of raw material and product baked with honey of
M. alba leaves as index. The quantitative analysis was carried out. RESULTS Twenty-three and twenty-four common peaks were
identified from the HPLC fingerprint spectra of ten batches of raw material and ten batches of product baked with honey of M. alba
leaves， respectively. The similarities of HPLC fingerprints for raw material and product baked with honey of M. alba leaves were
all greater than 0.97. The results of PCA showed that raw material and product baked with honey of M. alba leaves could be divided
into two categories. The results of OPLS-DA showed that the variable importance in projection of peak 2， peak H （5-
hydroxymethylfurfural）， peak 1， peak 17 （isochlorogenic acid C） and peak 16 were all greater than 1. The average contents of
differential marker of isochlorogenic acid C in raw material and product baked with honey of M. alba leaves were 0.093 6 and
0.127 8 mg/g， respectively； there was statistical significance （P＜0.05）. CONCLUSIONS Five differential markers such as
isochlorogenic acid C are obtained. The content of isochlorogenic acid C in M. alba leaves is significantly increased after baked
with honey.
KEYWORDS Morus alba leaves； honey-baked Morus alba leaves； fingerprint； chemometrics； differential marker
Δ 基金项目 山东省重点研发计划（重大科技创新工程项目）（No.
2021CXGC010511）；山东省中医药科技项目（No.M-2023078）；青岛市 桑叶为桑科植物桑 Morus alba L. 的干燥叶，其性
科技惠民示范专项（No.24-1-8-smjk-20-nsh） 寒、味甘苦，主要含黄酮、生物碱、氨基酸、多糖类等化学
＊第一作者 硕士研究生。研究方向：中药新药研发与炮制原理。 [1] [2]
成分 。桑叶生品长于疏散风热，清肝明目 ；桑叶蜜炙
E-mail：yanbeibei0518@163.com
后清燥润肺，可治外感燥热之身热头痛、口渴、干咳无
# 通信作者 主管药师，硕士。研究方向：中药制剂。E-mail：
madalong_1988@163.com 痰，可用于缓解气逆而喘、咽喉干燥等症状。目前，对桑
· 1198 · China Pharmacy 2024 Vol. 35 No. 10 中国药房 2024年第35卷第10期
