Page 28 - 《中国药房》2024年2期

光皮木瓜特征图谱及3种黄酮类成分含量测定研究 Δ

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朱田密 ，陈树和 ，严劲松 ，王新桂 ，段雨晴 ，杨孝义（1.湖北省中医院/湖北中医药大学附属医院/湖北省中
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医药研究院药事部，武汉 430061；2.湖北中医药大学药学院，武汉 430065）
中图分类号 R917 文献标志码 A 文章编号 1001-0408（2024）02-0150-05
DOI 10.6039/j.issn.1001-0408.2024.02.05
摘 要 目的 建立光皮木瓜的特征图谱，同时测定芦丁、金丝桃苷、槲皮苷 3 种黄酮类成分的含量，鉴别光皮木瓜和皱皮木瓜。
方法 采用高效液相色谱（HPLC）法。以 Agilent 5 TC-C18为色谱柱，以乙腈-0.2% 磷酸溶液为流动相进行梯度洗脱，流速为 1.0
mL/min，柱温为 30 ℃，特征图谱检测波长为 330 nm，含量测定检测波长为 350 nm。采用《中药色谱指纹图谱相似度评价系统
（2012版）》建立光皮木瓜的特征图谱并评价相似度，使用SPSS 23.0软件对15批光皮木瓜（S1～S15）进行聚类分析；测定15批光
皮木瓜和7批皱皮木瓜（S16～S22）中3种黄酮类成分的含量，比较二者特征图谱。结果 15批光皮木瓜样品的特征图谱相似度为
0.783～0.969；共确定11个特征峰；指认了4种成分，分别为绿原酸、芦丁、金丝桃苷、槲皮苷。15批光皮木瓜样品聚为2类，S5～S8
为一类，其余为一类。光皮木瓜与皱皮木瓜的特征图谱有明显区别。15 批光皮木瓜样品中芦丁、金丝桃苷、槲皮苷含量分别为
48.99～294.45、3.49～102.55、31.98～149.49 μg/g，皱皮木瓜中芦丁含量低于光皮木瓜，且皱皮木瓜中均未检出金丝桃苷（S20样品
除外）和槲皮苷。结论 所建立的光皮木瓜的特征图谱和 3 种黄酮类成分的含量测定方法可用于其质量控制及其与皱皮木瓜的
鉴别。
关键词 光皮木瓜；黄酮；特征图谱；高效液相色谱法

Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones
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ZHU Tianmi ，CHEN Shuhe ，YAN Jingsong ，WANG Xingui ，DUAN Yuqing ，YANG Xiaoyi （1. Dept. of
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Pharmacy， Hubei Provincial Hospital of Traditional Chinese Medicine/the Affiliated Hospital of Hubei University
of Chinese Medicine/Hubei Province Academy of Traditional Chinese Medicine， Wuhan 430061， China；2.
College of Pharmacy， Hubei University of Chinese Medicine， Wuhan 430065， China）
ABSTRACT OBJECTIVE To establish the characteristic chromatogram of Chaenomeles sinensis， determine the contents of
rutin， hyperin and quercitrin， and to identify C. sinensis and C. speciosa. METHODS HPLC method was performed on Agilent 5
TC-C18 column， with acetonitrile-0.2% formic acid solution as the mobile phase for gradient elution， at the flow rate of 1.0 mL/min.
The column temperature was 30 ℃. The detection wavelength was 330 nm in characteristic chromatogram and 350 nm in content
determination. The characteristic chromatogram of C. sinensis was established and similarity was evaluated by the Similarity
Evaluation System for Chromatographic Fingerprint of TCM （2012 edition）. Hierarchical cluster analysis of 15 batches of C. sinensis
（S1-S15） was performed by using SPSS 23.0 software. The contents of 3 flavones in 15 batches of C. sinensis and 7 batches of
C. speciosa （S16-S22） were determined， while their characteristic chromatograms were compared. RESULTS The similarities of
the characteristic chromatogram for 15 batches of C. sinensis ranged from 0.783 to 0.969， and 11 characteristic peaks were
confirmed. Four constituents were identified as chlorogenic acid， rutin， hyperin and quercitrin. The medicinal materials in 15
batches of C. sinensis could be divided into 2 categories： S5-S8 were one category， and the others belonged to one category. The
characteristic chromatogram of C. sinensis was obviously different from C. speciosa. The contents of rutin， hyperin and quercitrin in
15 batches of C. sinensis were 48.99-294.45， 3.49-102.55， 31.98-149.49 μg/g， respectively. The content of rutin in C. speciosa was
lower than that in C. sinensis. None of hyperin （except for S20） and quercitrin were detected in C. speciosa. CONCLUSIONS The
characteristic chromatogram and the method for content determination of 3 flavones in C. sinensis are established successfully and
can be used for the quality control of C. sinensis and its identification from C. speciosa.
KEYWORDS Chaenomeles sinensis； flavones； characteristic chromatogram； HPLC

光皮木瓜是蔷薇科木瓜属植物木瓜 Chaenomeles
Δ 基金项目 湖北省科技重大专项课题（No.2020ACA007-01） sinensis （Thouin） Koehne 的成熟或近成熟果实，又名榠
＊第一作者 副主任药师，硕士。研究方向：中药的品种与质量分 [1―2]
楂、木李 ，因果皮干燥后仍光滑，不皱，故称“光皮木
析。电话：027-88928141。E-mail：sweetness2001@126.com
[1]
瓜”。其果实清香，味酸、涩，性平，具有和胃舒筋、祛风
# 通信作者 主任药师。研究方向：医院中药制剂研发、中药饮片
[2]
质量控制。电话：027-88928141。E-mail：chenshuhe606@163.com 除湿、消痰止咳之功效 ，可药食两用，易与皱皮木瓜[蔷
· 150 · China Pharmacy 2024 Vol. 35 No. 2 中国药房 2024年第35卷第2期