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水冬瓜根及茎的质量控制方法研究
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          黎 雪    1， 2＊ ，聂雨杉 ，马 雪 ，陆 苑 ，杨 畅 ，李勇军 ，王永林 （1.贵州医科大学民族药与中药开发应用教
                           1， 2
                                     1
          育部工程研究中心/省部共建药用植物功效与利用国家重点实验室，贵阳 550004；2.贵州医科大学药学院，贵
          阳 550004；3.贵州医科大学贵州省药物制剂重点实验室，贵阳 550004）
          中图分类号  R284；R917      文献标志码  A      文章编号  1001-0408（2024）01-0021-06
          DOI  10.6039/j.issn.1001-0408.2024.01.04

          摘  要  目的  建立水冬瓜根及茎药材的质量控制方法。方法  观察水冬瓜根及茎药材的性状、显微特征；按 2020 年版《中国药
          典》（四部）通则方法对水冬瓜根及茎药材中的水分、总灰分、酸不溶性灰分和醇溶性浸出物进行测定；采用《中药色谱指纹图谱相
          似度评价系统（2012版）》建立11批水冬瓜根及茎药材的高效液相色谱（HPLC）指纹图谱，并进行共有峰的指认及相似度评价；采
          用HPLC法测定水冬瓜根及茎中松柏苷、紫丁香苷、绿原酸、（+）-syringaresinol-O-β-D-glucopyranoside、丁香脂素的含量。结果  水
          冬瓜根及茎药材性状与显微鉴别特征明显。水分、总灰分、酸不溶性灰分和醇溶性浸出物的含量平均值分别为 7.54%、2.18%、
          0.15%、7.81%。11批水冬瓜根及茎药材的指纹图谱有16个共有峰，相似度为0.856～0.960，共指认出松柏苷、紫丁香苷、绿原酸、
         （+）-syringaresinol-O-β-D-glucopyranoside、丁香脂素5个共有成分。上述5个成分的含量分别为0.047 2～0.401 6、0.836 8～8.697 9、
          1.245 3～10.950 0、0.139 0～0.437 8、0.016 4～0.635 3 mg/g。结论  所建方法稳定、准确性好，可用于水冬瓜根及茎药材的质量控
          制。初步拟定水冬瓜根及茎药材中水分不得过11.0%，总灰分不得过4.0%，醇溶性浸出物不得少于5.0%，松柏苷、紫丁香苷、绿原
          酸、（+）-syringaresinol-O-β-D-glucopyranoside、丁香脂素含量分别不得少于0.04、0.83、1.24、0.13、0.01 mg/g。
          关键词  水冬瓜根；水冬瓜茎；性状鉴别；显微鉴别；指纹图谱；含量测定；质量控制

          Study on quality control method of the roots and rhizoma of Toricellia angulata
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          LI Xue ，NIE Yushan ，MA Xue ，LU Yuan ，YANG Chang ，LI Yongjun ，WANG Yonglin（1.  Engineering
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          Research  Center  for  the  Development  and  Application  of  Ethnic  Medicine  and  TCM/State  Key  Laboratory  of
          Functions  and  Applications  of  Medicinal  Plants，  Guizhou  Medical  University，  Guiyang  550004，  China；
          2.  School  of  Pharmacy，  Guizhou  Medical  University，  Guiyang  550004，  China；3.  Guizhou  Provincial  Key
          Laboratory of Pharmaceutics， Guizhou Medical University， Guiyang 550004， China）
          ABSTRACT   OBJECTIVE To establish the quality control method for the roots and rhizoma of Toricellia angulata. METHODS
          The properties of the roots and rhizoma of T. angulata were observed and microscopic identification was conducted. The moisture，
          total  ash，  acid-insoluble  ash  and  ethanol-soluble  extract  were  examined  according  to  the  method  stated  in  the  2020  edition  of
          Chinese  Pharmacopoeia （part  Ⅳ）.  HPLC  fingerprints  of  11  batches  of  the  roots  and  rhizoma  of  T.  angulata  were  established，
          common  peaks  were  identified  and  the  similarity  was  evaluated  by  using  the  Similarity  Evaluation  System  of  Chromatographic
          Fingerprint of TCM （2012 edition）. The contents of coniferin， syringin， chlorogenic acid， （+）-syringaresinol-O-β-D-glucopyranoside
          and syringaresinol were determined by HPLC. RESULTS The properties and microscopic identification of the roots and rhizoma of
          T. angulata were obvious. The average contents of moisture， total ash， acid-insoluble ash and ethanol-soluble extract were 7.54%，
          2.18%， 0.15% and 7.81%， respectively. There were 16 common peaks marked in the HPLC fingerprints of 11 batches of the roots
          and rhizoma of T. angulata， with similarities of 0.856-0.960； five of them were identified， such as coniferin， syringin， chlorogenic
          acid， （+）-syringaresinol-O-β-D-glucopyranoside and syringaresinol. The contents of the above five components were 0.047 2-0.401 6，
          0.836  8-8.697  9，  1.245  3-10.950  0，  0.139  0-0.437  8  and  0.016  4-0.635  3  mg/g，  respectively.  CONCLUSIONS  The  established
          method is stable and accurate， which can be used for the quality control of the roots and rhizoma of T. angulata. It is preliminarily
          proposed that the moisture in the roots and rhizoma of T. angulata is not more than 11.0%， the total ash is not more than 4.0%， the
          ethanol-soluble  extract  is  not  less  than  5.0%，  the  contents  of  coniferin，  syringin，  chlorogenic  acid， （+）-syringaresinol-O-β-D-
                                                             glucopyranoside  and  syringaresinol  are  not  less  than  0.04，
             Δ 基金项目 国家自然科学基金委员会-贵州省人民政府联合基金
                                                             0.83， 1.24， 0.13， 0.01 mg/g， respectively.
          项目（No.U1812403）；贵州省高层次创新型人才培养计划——“百”层              KEYWORDS     roots  of  Toricellia  angulata；  rhizoma  of
          次人才项目（No.黔科合平台人才〔2016〕5677）                        Toricellia  angulata；  property  identification；  microscopic
             ＊第一作者 硕士研究生。研究方向：药物新剂型、新技术、新工艺
                                                             identification；  fingerprint；  content  determination；  quality
          与新药研发。E-mail：3224696624@qq.com
                                                             control
             # 通信作者 教授，博士生导师。研究方向：中药药效物质基础及
          新药开发。E-mail：gywyl@gmc.edu.cn


          中国药房  2024年第35卷第1期                                                  China Pharmacy  2024 Vol. 35  No. 1    · 21 ·
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