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玉屏风颗粒通过调节EMT对Lewis荷瘤小鼠肿瘤转移的影响
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林映雪 ，崔海鹏 ，王领弟 ，杨昌硕 ，庄朋伟 （1.承德医学院附属医院药学部，河北 承德 067000；2.承德医
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学院病理生理学教研室，河北 承德 067000；3.天津中医药大学中药学院，天津 300193）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2023）17-2090-05
DOI 10.6039/j.issn.1001-0408.2023.17.08
摘 要 目的 探讨玉屏风颗粒通过调节上皮-间充质转化（EMT）改善上皮屏障功能并抑制肿瘤转移的作用。方法 将 30 只
C57BL/6小鼠按随机数字表法分为正常组（蒸馏水）、模型组（蒸馏水）和玉屏风颗粒组（40 g/kg），每组10只。模型组与玉屏风颗
粒组小鼠通过右腋皮下接种Lewis肺癌细胞复制小鼠自发肺转移模型。造模成功后，各组小鼠灌胃水/药物，每天1次，连续15 d。
通过观察/测定小鼠肺组织病理形态、肺组织肿瘤转移灶计数、肿瘤转移灶、癌胚抗原表达来考察玉屏风颗粒对肿瘤转移的影响；
通过qRT-PCR法和Western blot法检测小鼠肺组织中β-连环蛋白（β-catenin）、E-钙黏蛋白（E-cadherin）、波形蛋白（vimentin）mRNA
及其蛋白表达来考察可能的作用机制。结果 与模型组比较，玉屏风颗粒组小鼠肺表面转移结节总数显著减少（P＜0.05），肺组织
大体形态有所恢复，肺组织癌胚抗原表达显著减弱（P＜0.05），且肺组织中E-cadherin mRNA及其蛋白表达水平均显著升高（P＜
0.05），vimentin、β-catenin mRNA及其蛋白表达水平均显著降低（P＜0.05）。结论 玉屏风颗粒可能是通过调控β-catenin表达来抑
制EMT的发生，进而改善小鼠的上皮屏障功能，发挥抑制肿瘤细胞侵袭、迁移的作用。
关键词 玉屏风颗粒；上皮-间充质转化；上皮屏障；肿瘤转移；β-连环蛋白
Effects of Yupingfeng granules on tumor metastasis of Lewis tumor-bearing mice by adjusting the EMT
LIN Yingxue ，CUI Haipeng ，WANG Lingdi ，YANG Changshuo ，ZHUANG Pengwei（1. Dept. of Pharmacy，the
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Affiliated Hospital of Chengde Medical University，Hebei Chengde 067000，China；2. Teaching and Research
Section of Pathophysiology，Chengde Medical University，Hebei Chengde 067000，China；3. School of Chinese
Materia Medica，Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China）
ABSTRACT OBJECTIVE To explore the effects of Yupingfeng granules on the improvement of epithelial barrier function and
the inhibition of tumor metastasis by regulating epithelial-mesenchymal transition （EMT）. METHODS Thirty C57BL/6 mice were
randomly divided into the normal group （distilled water），model group （distilled water） and Yupingfeng granule group （40 g/kg），
with 10 mice in each group. The model group and Yupingfeng granule group were inoculated with Lewis lung cancer cells
subcutaneously in the right armpit to induce the spontaneous lung metastasis model. After modeling，each group was given water/
relevant medicine intragastrically，once a day，for 15 consecutive days. The effects of Yupingfeng granules on tumor metastasis were
investigated by observing or determining the pathomorphology of lung tissue，metastatic lesion count on the surface of the lung，
tumor metastatic lesion and the expression of carcinoembryonic antigens. qRT-PCR and Western blot assay were used to detect the
mRNA and protein expressions of β-catenin，E-cadherin and vimentin in the lung tissue of mice. RESULTS Compared with the
model group，the total number of pulmonary metastases on the surface was decreased significantly in Yupingfeng granule group （P＜
0.05），the general morphology of lung tissue was recovered，and the expression of carcinoembryonic antigen in lung tissue was
significantly decreased （P＜0.05）. mRNA and protein expressions of E-cadherin in lung tissue were significantly increased （P＜
0.05），while mRNA and protein expressions of vimentin and β-catenin were significantly decreased （P＜0.05）. CONCLUSIONS
Yupingfeng granules can inhibit EMT by regulating the expression of β -catenin，thus improving epithelial barrier function，and
inhibiting the ability of tumor cells to invade and metastasize.
KEYWORDS Yupingfeng granules； epithelial-mesenchymal
Δ 基金项目 天津市自然科学基金项目（No.18JCZDJC99300）；河 transition； epithelial barrier； tumor metastasis； β-catenin
北省中医药管理局科研计划项目（No.2022152）；河北省医学科学研究
课题（No.20200183）；承德医学院校级科研课题（No.202213）
＊第一作者 主管中药师，硕士。研究方向：中西医结合与临床精 上 皮 - 间 充 质 转 化（epithelial-mesenchymal transi‐
准用药。E-mail：linyxsun@163.com
tion，EMT）是上皮细胞失去极性，并转化成间质细胞表
# 通信作者 教授，博士。研究方向：中药药效特点及药物安全性
评价关键技术。E-mail：zhuangpengwei@163.com 型的过程。EMT可破坏上皮屏障功能的完整性，诱发恶
· 2090 · China Pharmacy 2023 Vol. 34 No. 17 中国药房 2023年第34卷第17期
