Page 67 - 《中国药房》2022年20期

柴胡陷胸汤含药血清对高脂血清致人脐静脉内皮细胞损伤的影

响及机制 Δ

3 #
王建湘 ，廖 杨 ，易 琼 ，陈新宇 （1.湖南中医药大学第一附属医院重症医学科，长沙 410007；2.湖南中医
1＊
1
2
药大学研究生院，长沙 410208；3.湖南中医药大学第一附属医院心血管内科，长沙 410007）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2022）20-2493-06
DOI 10.6039/j.issn.1001-0408.2022.20.12

摘 要 目的 探究柴胡陷胸汤含药血清对高脂血清致人脐静脉内皮细胞ECV304损伤的影响及机制。方法 制备高脂血清和柴
胡陷胸汤含药血清。以高脂血清诱导ECV304细胞损伤，并以低、中、高浓度（3.47、6.94、13.88 g/kg，以生药量计）的柴胡陷胸汤含
药血清进行干预，检测各组细胞增殖率和细胞上清液中相关炎症因子[肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）]、血管内皮
功能指标[内皮型一氧化氮合酶（eNOS）、一氧化氮（NO）、内皮素1（ET-1）]水平，检测细胞中细胞间黏附分子1（ICAM-1）、转化生
长因子β1 （TGF-β1 ）mRNA的表达水平以及Toll样受体4（TLR4）、核因子κB抑制因子α（IκBα）、磷酸化IκBα（p-IκBα）蛋白的表达
水平和磷酸化核因子κB p65（p-NF-κB p65）/NF-κB p65比值。结果 与对照组比较，模型组细胞增殖率，TNF-α、IL-6、ET-1水平，
ICAM-1 mRNA 表达水平，TLR4、p-IκBα 蛋白表达水平和 p-NF-κB p65/NF-κB p65 比值均显著升高（P＜0.05），eNOS、NO 水平，
TGF-β1 mRNA表达水平和IκBα蛋白表达水平均显著降低（P＜0.05）；与模型组比较，柴胡陷胸汤低、中、高浓度组上述指标均显著
好转（P＜0.05），且柴胡陷胸汤高浓度组显著优于柴胡陷胸汤低、中浓度组（P＜0.05）。结论 柴胡陷胸汤可改善高脂血清诱导的
血管内皮细胞损伤，其作用机制可能与降低炎症因子水平、抑制TRL4/NF-κB通路活化有关。
关键词 柴胡陷胸汤；含药血清；高脂血清；内皮细胞损伤；炎症反应；Toll样受体4/核因子κB通路

Effects of Chaihu xianxiong decoction containing serum on HUVEC injury induced by high-fat serum and
its mechanism
WANG Jianxiang ，LIAO Yang ，YI Qiong ，CHEN Xinyu（1. Dept. of Critical Care Medicine，the First Affiliated
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Hospital of Hunan University of Chinese Medicine，Changsha 410007，China；2. School of Graduate，Hunan
University of Chinese Medicine，Changsha 410208，China；3. Dept. of Cardiovascular Medicine，the First
Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，China）
ABSTRACT OBJECTIVE To explore the effects of Chaihu xianxiong decoction containing serum on human umbilical vein
endothelial cells ECV304 injury induced by high-fat serum and its mechanism. METHODS The high-fat serum and Chaihu
xianxiong decoction containing serum were prepared. ECV304 injury was induced by high-fat serum，and the intervention
experiments were conducted with low-concentration，medium-concentration and high-concentration （3.47，6.94，13.88 g/kg，by
crude drug） of Chaihu xianxiong decoction containing serum. The proliferation rate，the levels of related inflammatory factors
[tumor necrosis factor-α（TNF-α），interleukin-6（IL-6）] and vascular endothelial function indexes [endothelial nitric oxide synthase
（eNOS），nitric oxide（NO）and endothelin-1（ET-1）] were all detected in each group. mRNA expressions of intercellular adhesion
molecule-1 （ICAM-1） and transforming growth factor- β1 （TGF- β1 ） were detected，and the expression of Toll-like receptor 4
（TLR4），nuclear factor-κB inhibitor α（IκBα），phosphorylated IκBα（p-IκBα）protein and the ratio of phosphorylated nuclear
factor-κB p65（p-NF-κB p65）to NF-κB p65 were also detected. RESULTS Compared with control group，the proliferation rate of
cell，the levels of TNF-α，IL-6 and ET-1，mRNA expression of ICAM-1，protein expressions of TLR4 and p-IκBα and p-NF-κB
p65/NF-κB p65 ratio were significantly increased in the model group（P＜0.05）；the levels of eNOS and NO，mRNA expression of
TGF-β1 and protein expression of IκBα were significantly decreased（P＜0.05）. Compared with model group，above indexes were
all reversed significantly in low-concentration，medium-concentration and high-concentration groups of Chaihu xianxiong decoction
（P＜0.05）， while the Chaihu xianxiong decoction high-
Δ 基金项目 湖南省教育厅科学研究项目（No.17C1223）
concentration group were all better than low-concentration and
＊第一作者 副主任医师，博士。研究方向：中医药防治心血管疾
病。E-mail：wang_w00@163.com medium-concentration groups （P＜0.05）. CONCLUSIONS
# 通信作者 主任医师，博士生导师，博士。研究方向：中医药防治 Chaihu xianxiong decoction can improve endothelial cell injury
心脑血管疾病。E-mail：chen_c123@126.com induced by high-fat serum. Its mechanism of action may be

中国药房 2022年第33卷第20期 China Pharmacy 2022 Vol. 33 No. 20 ·2493·