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清解化攻方调节重症急性胰腺炎模型大鼠肠道菌群及对肠黏膜

        屏障的影响             Δ


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        秦百君   1，2＊ ，唐曦平 ，杨 昕 ，杨 蕾 ，冯敏超 ，张 驰 ，洪晓华 ，蓝艳梅 ，陈国忠 （1.广西中医药大学第一附
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        属医院脾胃科，南宁 530023；2.广西中医药大学第一临床医学院，南宁 530001；3.广西医科大学附属肿瘤医
        院胰腺内科，南宁 530021；4.仁寿县中医院内科，四川 眉山 620500）
        中图分类号 R965          文献标志码 A          文章编号 1001-0408（2022）15-1825-08
        DOI  10.6039/j.issn.1001-0408.2022.15.07
        摘  要   目的 研究清解化攻方调节重症急性胰腺炎（SAP）模型大鼠肠道菌群及对肠黏膜屏障的影响。方法 采用雨蛙素联合脂
        多糖腹腔注射法复制SAP大鼠模型，观察各组大鼠生存状态，检测大鼠血清中血清淀粉酶、白细胞介素10（IL-10）、IL-18、IL-1β水
        平，观察大鼠胰腺及小肠组织的病理变化，检测大鼠小肠组织中闭合蛋白（Occludin）、闭锁连接蛋白1（ZO-1）、高迁移率族蛋白B1
       （HMGB1）的表达水平；利用16S rRNA高通量测序检测大鼠肠道菌群结构和相对丰度。结果 经清解化攻方干预后，SAP模型大
        鼠腹部膨胀症状明显减轻，精神状态恢复较好；血清中血清淀粉酶、IL-18水平显著降低（P＜0.05），IL-10水平显著升高（P＜0.05）；
        胰腺组织坏死区域、炎症细胞浸润减少，肠上皮细胞结构紊乱程度有所缓解，肠黏膜上皮脱落减少；小肠组织中HMGB1蛋白表达
        水平显著降低（P＜0.05），Occludin、ZO-1蛋白表达水平均显著升高（P＜0.05）。16S rRNA高通量测序结果显示，清解化攻方可升
        高大鼠肠道拟杆菌门、乳酸杆菌属等益生菌群的相对丰度，减少厚壁菌门等有害菌群的定植。结论 清解化攻方可通过上调小肠
        组织Occludin、ZO-1蛋白表达，下调HMGB1蛋白表达，改善肠黏膜屏障损伤，并可调节不同菌群的相对丰度从而达到保护肠道的
        作用。
        关键词 重症急性胰腺炎；清解化攻方；肠道菌群；肠黏膜屏障

        Study on the effects of Qingjie huagong decoction on the regulation of intestinal flora and intestinal
        mucosal barrier in severe acute pancreatitis model rats
        QIN Baijun ，TANG Xiping ，YANG Xin ，YANG Lei ，FENG Minchao ，ZHANG Chi ，HONG Xiaohua ，LAN Yanmei ，CHEN
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        Guozhong（1. Dept. of Spleen and Stomach，the First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning
        530023，China；2. First School of Clinical Medicine，Guangxi University of Chinese Medicine，Nanning 530001，China；3. Dept. of
        Pancreas Internal Medicine，the Affiliated Cancer Hospital of Guangxi Medical University，Nanning 530021，China；4. Dept. of
        Internal Medicine，Renshou County Hospital of TCM，Sichuan Meishan 620500，China）
        ABSTRACT   OBJECTIVE To explore the the regulation of intestinal flora and effects of Qingjie huagong decoction on intestinal
        mucosal barrier in severe acute pancreatitis（SAP）mode rats. METHODS SAP rat model was induced by intraperitoneal injection
        of caerulein and lipopolysaccharide.The survival state of rats in each group were observed.The levels of serum amylase，interleukin
        10（IL-10），IL-18 and IL-1β in serum were all detected. The pathological changes of pancreatic and small intestinal tissue were
        observed. The expressions of Occludin，ZO-1 and HMGB1 were detected in small intestinal tissue of rats. The structure and relative
        abundance of intestinal microflora in rats were detected by 16S rRNA high throughput sequencing. RESULTS After the intervention
        of Qingjie huagong decoction，abdominal distension symptoms of SAP model rats were significantly relieved，and their mental state
        recovered better；the levels of serum amylase and IL-18 in serum were decreased significantly（P＜0.05），while the level of IL-10
        was increased significantly（P＜0.05）. The necrotic area of pancreatic tissue and the infiltration of inflammatory cells were reduced，
        the degree of intestinal epithelial cell structural disorder was alleviated，and the shedding of intestinal mucosal epithelium was
        reduced.The protein expression of HMGB1 in small intestinal tissue was decreased significantly （P＜0.05），and the protein
                                                           expression of Occludin and ZO-1 were increased significantly
           Δ 基金项目 国家自然科学基金资助项目（No.82160890）；广西医            （P＜0.05）. Results of 16S rRNA high throughput sequencing
        疗卫生适宜技术开发与推广应用项目（No.S2019021）
                                                           showed that Qingjie huagong decoction could increased the
           ＊第一作者 博士研究生。研究方向：中医药防治消化系统疾病的
                                                           relative abundance of probiotics such as Bacteroidea and
        临床及基础研究。E-mail：2650658716@qq.com
           # 通信作者 教授，主任医师，博士生导师。研究方向：中医药防治                 Lactobacillus in rat intestine，reduced the colonization of
        消化系统疾病的药理及机制。E-mail：chenin-jp@163.com              harmful bacteria such as Firmicutes. CONCLUSIONS Qingjie


        中国药房    2022年第33卷第15期                                             China Pharmacy 2022 Vol. 33 No. 15  ·1825 ·
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