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枸杞类胡萝卜素指纹图谱的建立及其抗氧化活性谱效关系研究 Δ

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王 静 ，王 杰，阿娜尔，李 彤，杨冬平，戴俊东，董 玲（北京中医药大学生命科学院，北京 102488）
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中图分类号 R284.1 文献标志码 A 文章编号 1001-0408（2022）05-0575-04
DOI 10.6039/j.issn.1001-0408.2022.05.11

摘 要 目的 建立枸杞类胡萝卜素高效液相色谱（HPLC）指纹图谱，并考察其共有峰与抗氧化活性的谱效关系。方法 采用
HPLC法进行检测，根据《中药指纹图谱相似度评价系统》（2012版）建立34批不同产地枸杞类胡萝卜素的指纹图谱，并进行相似度
评价和共有峰指认。以1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除率为指标，考察枸杞类胡萝卜素的体外抗氧化活性，并采用
灰色关联度法分析枸杞类胡萝卜素各共有峰与抗氧化活性的谱效关系。结果 34批枸杞类胡萝卜素共有4个共有峰，相似度均不
低于0.903；并指认出1号峰为玉米黄质、4号峰为玉米黄质双棕榈酸酯。其对DPPH自由基的清除率为1.792％～3.160％。枸杞
类胡萝卜素各共有峰与DPPH自由基清除率均呈正相关，关联度均大于0.6；2号峰、4号峰（玉米黄质双棕榈酸酯）与DPPH自由基
清除率的关联度均大于0.8。根据关联度确定各共有峰对DPPH自由基清除率的贡献大小依次为2号峰＞4号峰（玉米黄质双棕榈
酸酯）＞1号峰（玉米黄质）＞3号峰。结论 本研究成功建立了枸杞类胡萝卜素的HPLC指纹图谱，指认了2个共有峰，其中2号峰
所代表化学成分和玉米黄质棕榈酸酯可能是枸杞类胡萝卜素抗氧化活性的物质基础。
关键词 枸杞；类胡萝卜素；指纹图谱；谱效关系

Establishment of carotenoid fingerprint in Lycium barbarum and study on its antioxidant activity
spectrum-effect relationship
WANG Jing，WANG Jie，Anaer，LI Tong，YANG Dongping，DAI Jundong，DONG Ling（College of Life Science，
Beijing University of Chinese Medicine，Beijing 102488，China）

ABSTRACT OBJECTIVE To establish the high performance liquid chromatography（HPLC）fingerprint of carotenoid in Lycium
barbarum，and to investigate the spectrum-effect relationship between its common peak and antioxidant activity. METHODS HPLC
method was adopted. The fingerprints of carotenoid in 34 batches of L. barbarum from different producing areas were established
by Similarity Evaluation System of TCM Fingerprint（2012 edition），and similarity evaluation and common peak identification were
carried out. Taking scavenging rate of DPPH free radical as index，in vitro antioxidant activity of carotenoid in L. barbarum was
investigated. The spectrum-effect relationship between the common peaks of carotenoids in L. barbarum and antioxidant activity was
analyzed by grey correlation method. RESULTS There were 4 common peaks in the fingerprints of carotenoids in 34 batches of
L. barbarum，and the similarity was not less than 0.903. Peak 1 was identified as zeaxanthin，and peak 4 as zeaxanthin dipalmitate.
The scavenging rates of them to DPPH free radical were 1.792％-3.160％. The common peaks of carotenoids in L. barbarum were
positively correlated with scavenging rate of DPPH free radical，and the correlation degree was greater than 0.6；the correlation
degree of peak 2 and peak 4（zeaxanthin dipalmitate）with scavenging rate of DPPH free radical was greater than 0.8. According to
the correlation degree，the contribution of each common peak to scavenging rate of DPPH free radical was determined as peak 2＞
peak 4（zeaxanthin dipalmitate）＞peak 1（zeaxanthin）＞peak 3. CONCLUSIONS In this study，HPLC fingerprint of carotenoid in
L. barbarum is successfully established，and two common peaks are identified. The chemical components represented by peak 2 and
zeaxanthin palmitate may be the material basis of antioxidant activity of carotenoid in L. barbarum.
KEYWORDS Lycium barbarum；carotenoid；fingerprint；spectrum-effect relationship

[1]
枸杞为茄科植物宁夏枸杞 Lycium barbarum L.的干 人长寿” ，其药用价值备受历代医家的推崇。现代研
燥成熟果实，是我国传统名贵中药材，具有悠久的药用 究发现，枸杞中主要活性成分为多糖、类胡萝卜素及其
历史。《本草纲目》载曰：“枸杞，补肾生精，养肝，明目，令 衍生物，其次还有生物碱类、黄酮类化合物 [2－3] 。现代药
Δ 基金项目：宁夏回族自治区重点研发计划重大（重点）项目 理学研究表明，枸杞的活性成分具有抑菌、降血脂、降血
[4]
（No.2018BCF01002） 糖、抗衰老等药理作用 。其中，枸杞类胡萝卜素具有清
＊硕士。研究方向：中药标准化。E-mail：17864189673@163.
除羟自由基、超氧自由基和过氧化氢的能力 [5－7] 。但是，
com
具体是枸杞类胡萝卜素中哪种成分发挥上述作用，尚不
# 通信作者：研究员，博士生导师，博士。研究方向：中药标准
化。E-mail：dongling@bucm.edu.cn 明确。

中国药房 2022年第33卷第5期 China Pharmacy 2022 Vol. 33 No. 5 ·575 ·