Page 63 - 《中国药房》2022年2期

蛇床子药材指纹图谱及4种香豆素类成分含量测定方法的建立 Δ

潘晓君 ，吕渭升，杨文惠，罗宇琴，梁月仪，魏 梅，孙冬梅，陈向东，霍文杰，李振雨（广东省中药配方颗粒企业
#
＊
重点实验室，广东 佛山 528244）

中图分类号 R284 文献标志码 A 文章编号 1001-0408（2022）02-0185-06
DOI 10.6039/j.issn.1001-0408.2022.02.10

摘 要 目的 建立蛇床子药材的指纹图谱及其中4种香豆素类成分的含量测定方法。方法 采用超高效液相色谱法，利用《中药
色谱指纹图谱相似度评价系统（2012版）》建立21批蛇床子药材的指纹图谱，并进行相似度评价，与对照品比对指认共有峰。以10
个共有峰峰面积为变量，采用组间连接法对21批蛇床子药材进行系统聚类分析。采用相同超高效液相色谱法，以蛇床子素为内
参物，计算花椒毒素、佛手柑内酯、欧前胡素的相对校正因子，采用一测多评法计算其含量，并与外标法测定结果进行比较。结果
21批蛇床子药材的指纹图谱中共标识出10个共有峰，相似度为0.997～1.000；指认出峰4为花椒毒素，峰8为佛手柑内酯，峰9为
欧前胡素，峰10为蛇床子素。21批样品被划分为3类，其中S7为一类，S14为一类，其余19批为一类。一测多评法与外标法测定
的花椒毒素、佛手柑内酯、欧前胡素含量的相对偏差分别为0.88％～1.07％、2.22％～2.29％、0.67％～2.93％。结论 成功建立了蛇
床子药材的超高效液相色谱指纹图谱及4种香豆素类成分含量测定的一测多评法。
关键词 蛇床子；超高效液相色谱法；指纹图谱；香豆素类成分；一测多评法

Establishment of the fingerprint of Cnidium monnieri and a method for the content determination of 4
kinds of coumarins
PAN Xiaojun，LYU Weisheng，YANG Wenhui，LUO Yuqin，LIANG Yueyi，WEI Mei，SUN Dongmei，
CHEN Xiangdong，HUO Wenjie，LI Zhenyu（Guangdong Provincial Enterprise Key Laboratory of Traditional
Chinese Medicine Formula Granule，Guangdong Foshan 528244，China）

ABSTRACT OBJECTIVE To establish the fingerprint of Cnidium monnieri and a method for the content determination of 4
kinds of coumarins. METHODS Ultra-high performance liquid chromatography（UPLC） method was adopted to establish the
fingerprints of 21 batches of C. monnieri； their similarities were evaluated with Similarity Evaluation System of TCM
Chromatographic Fingerprint（2012 edition）；common peaks were identification by comparison with reference substance. Using 10
common peak areas as variables，cluster analysis was performed for 21 batches of C. monnieri by the method of between groups.
The relative correction factors of xanthotoxin，bergapten and imperatorin were calculated by the same UPLC method with osthole
as the internal reference. The contents of them were calculated by quantitative analysis of multi-components by single marker
（QAMS），and compared with the results of external standard method. RESULTS Totally 10 common peaks were identified in the
fingerprints of 21 batches of C. monnieri；the similarities ranged from 0.997 to 1.000. Peak 4 was identified as xanthotoxin，peak 8
as bergapten，peak 9 as imperatorin and peak 10 as osthole. A total of 21 batches of samples were divided into 3 categories，of
which S7 was clustered into one category，S14 was clustered into one category，and the other 19 batches were clustered into one
category. The relative deviations of the contents of xanthotoxin，bergapten and imperatorin determined by QAMS and external
standard method were in the range of 0.88％ -1.07％ ，2.22％ -2.29％ ，0.67％ -2.93％ ，respectively. CONCLUSIONS UPLC
fingerprint of C. monnieri is successfully established，and QAMS method for content determination of 4 coumarins is also established.
KEYWORDS Cnidium monnieri；ultra-high performance liquid chromatography；fingerprint；coumarins；quantitative analysis of
multi-components by single marker

[3]
蛇床子为伞形科植物蛇床 Cnidium monnieri（L.） 主 。有研究发现，不同产地蛇床子所含香豆素的种类
Cuss.的干燥成熟果实 ，又名蛇粟、蛇米、蛇珠等，主要分 相差不大，但不同类型香豆素的含量差异明显，这种差
[1]
[2]
布于我国江苏、山东、浙江、陕西等地 。蛇床子的主要 异与不同产地温度、降雨量和日照时长的相关性较
[4]
活性成分为香豆素类，以简单型香豆素（如蛇床子素）和 高 。现代药理学研究表明，香豆素类成分是蛇床子的
呋喃型香豆素（如佛手苷内酯、花椒毒素、欧前胡素）为 主要活性成分，可有利于心脑血管系统、神经系统、免疫
系统、生殖系统等疾病以及骨质疏松的治疗 [5－8] 。2020
Δ 基金项目：广东省省级科技计划项目（No.2018B030323004）；
年版《中国药典》（一部）以蛇床子素为单一含量测定指
“广东特支计划”科技创业领军人才项目（No.2017TY04R197）
标，难以全面地反映蛇床子药材的内在质量；而多指标
＊学士。研究方向：中药饮片及中药配方颗粒的质量控制。电
检测则常因对照品昂贵或不易获得，导致检测成本较
话：0757-85128602。E-mail：947443994@qq.com
# 通信作者：主管中药师，硕士。研究方向：中药饮片及中药配方 高。一测多评法可采用同一对照品实现对多种成分的
颗粒。电话：0757-85128602。E-mail：1083656123@qq.com 同时测定，实用性和经济性较强。基于此，本文以超高

中国药房 2022年第33卷第2期 China Pharmacy 2022 Vol. 33 No. 2 ·185 ·