Page 100 - 2021年19期

血浆标本病毒灭活处理对伏立康唑、利奈唑胺、万古霉素和替考

拉宁血药浓度检测的影响 Δ

＊
李红莲 ，周 琼，张 峻，黄 桦，王晶晶，姚 勤（昆明医科大学第一附属医院临床药学科，昆明 650000）
#
中图分类号 R917 文献标志码 A 文章编号 1001-0408（2021）19-2394-06
DOI 10.6039/j.issn.1001-0408.2021.19.15

摘 要 目的：研究血浆标本病毒灭活处理对伏立康唑、利奈唑胺、万古霉素和替考拉宁血药浓度检测的影响。方法：以我院36
例住院患者分别行常规伏立康唑、利奈唑胺、万古霉素和替考拉宁血药浓度检查后剩余的血浆为检测标本（每种药物的含药血浆
均为9份），根据各药物的常规血药浓度检测结果按3个一组将其分别分为低、中、高3个浓度水平的混合标本；然后将不同浓度水
平的混合标本分别分为灭活组和非灭活组，每组3份。灭活组标本采用56 ℃干式恒温金属浴加热30 min进行灭活处理，非灭活
组标本不作处理。两组血浆样本分别进行前处理后，采用二维液相色谱法分别测定4种药物的血药浓度，比较其检测结果差异。
结果：含伏立康唑、利奈唑胺、万古霉素和替考拉宁的血浆标本经56 ℃干式恒温金属浴加热30 min灭活后依然较稳定；相较于非
灭活组，灭活组低、中、高浓度水平混合血浆样本中上述4种药物的血药浓度检测结果的相对误差均小于15％。结论：采用二维液
相色谱法测定血浆标本中伏立康唑、利奈唑胺、万古霉素和替考拉宁的血药浓度时，可采用56 ℃干式恒温金属浴加热30 min的方
法对血浆标本进行灭活。
关键词 伏立康唑；利奈唑胺；万古霉素；替考拉宁；血药浓度；血浆标本；病毒灭活

Effects of Virus Inactivation Treatment of Plasma Specimen on Plasma Concentration Determination of
Voriconazole，Linezolid，Vancomycin and Teicoplanin
LI Honglian，ZHOU Qiong，ZHANG Jun，HUANG Hua，WANG Jingjing，YAO Qin（Dept. of Clinical Pharmacy，
the First Affiliated Hospital of Kunming Medical University，Kunming 650000，China）

ABSTRACT OBJECTIVE：To study the effects of virus inactivation treatment of plasma specimen on plasma concentration
determination of voriconzole，linezolid，vancomycin and teicoplanin. METHODS：The remaining plasma of 36 inpatients in our
hospital after routine blood concentration examination of voriconazole，linezolid，vancomycin and teicoplanin were collected as
specimen（9 drug-contained plasma specimens for each drug），and merged into three different concentration levels（low，medium，
high）of mixed samples according the results of routine blood test. Then the mixed samples with different concentration levels were
divided into inactivated group and non-inactivated group，with 3 samples in each group. The inactivated plasma samples were
heated at 56 ℃ for 30 min in metal bath with constant temperature. Non-inactivated group were not treated. After pretreating plasma
sample of 2 groups，2-dimensional liquid chromatography was used to detect plasma concentration of the four drugs；the difference
of detection result between inactivated group and non-inactivated group were analyzed. RESULTS：Plasma samples containing
voriconazole，linezolid，vancomycin and teicoplanin were still stable after heating at 56 ℃ for 30 min in metal bath with constant
temperature. Compared with non-inactivated group，relative error of plasma concentration detection result of above 4 drugs were all
lower than 15％ in low，medium，high concentration mixed samples of inactivated group. CONCLUSIONS：Plasma samples can
be inactivated by heating at 56 ℃ for 30 min in metal bath with constant temperature，when the plasma concentration of
voriconazole，linezolid，vancomycin and teicoplanin are determined by 2-dimensional liquid chromatography.
KEYWORDS Voriconazole；Linezolid；Vancomycin；Teicoplanin；Plasma concentration；Plasma sample；Virus inactivation

病毒的侵染会严重威胁人类的健康和生命安全，如 毒引发的新型冠状病毒肺炎等均造成了大量人口死
非典型病原体引发的非典型病原体肺炎和新型冠状病 亡 [1－2] 。此外，人类免疫缺陷病毒、流感病毒及狂犬病毒
等也长期威胁着人类的生命健康 。接触传播、呼吸道
[3]
Δ 基金项目：云南省卫生和计划生育委员会医学领军人才培养计
划项目（No.L201614）；云南省高层次卫生计生技术人才培养项目（No. 飞沫传播、分泌物传播、唾液传播、血液传播和污染品传
H-2017051，No.D-2018043）；昆明医科大学研究生创新基金（No.2020- 播等均为病毒的常见传播途径 [4－6] 。
S119）
病毒感染可能会导致机体免疫力低下和多器官衰
＊硕士。研究方向：临床药学。E-mail：2736553403@qq.com
竭，故病毒感染患者常合并真菌及细菌感染，临床上常
# 通信作者：副主任药师，硕士。研究方向：体内药物分析。电
话：0871-65324888。E-mail：23357999@qq.com 使用抗菌药物进行辅助治疗 [1－2] 。伏立康唑、利奈唑胺、

·2394 · China Pharmacy 2021 Vol. 32 No. 19 中国药房 2021年第32卷第19期