Page 43 - 2021年17期

不同产地铁线蕨药材指纹图谱的建立、分析及差异成分的含量测

定 Δ

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肖 丹 ，范东生 ，刘 星 ，孔 毅 ，雪锦菁 ，高 源 （1.贵州省食品检验检测院，贵阳 550004；2.贵州中医
药大学第一附属医院药剂科，贵阳 550001）
中图分类号 R284.1 文献标志码 A 文章编号 1001-0408（2021）17-2085-06
DOI 10.6039/j.issn.1001-0408.2021.17.07
摘 要 目的：建立不同产地铁线蕨药材的指纹图谱，并进行化学计量学分析和差异成分的含量测定，为铁线蕨药材质量控制提
供参考。方法：采用高效液相色谱-二极管阵列检测器法（HPLC-DAD）检测并结合《中药色谱指纹图谱相似度评价系统》（2004A
版）建立 19 批不同产地铁线蕨药材（S1～S19）的指纹图谱，同时进行共有峰指认和相似度评价。采用聚类分析、主成分分析
（PCA）、正交偏最小二乘法-判别分析（OPLS-DA）等化学计量学分析方法对19批不同产地铁线蕨药材的质量进行评价，筛选差异
成分，并对部分差异成分进行含量测定。结果：19批不同产地铁线蕨药材中，共确定22个共有峰，并指认了9号峰为绿原酸、17号
峰为槲皮素-3-O-β-D-吡喃葡糖苷酸、20号峰为山柰酚-3-O-芸香糖苷；19批药材的相似度为0.530～0.962。经聚类分析发现，S7、
S10聚为一类，S15、S18聚为一类，S1～S6、S8、S9、S11～S14、S16、S17、S19聚为一类；经PCA、OPLS-DA 发现，S7、S10聚为一类，
S15为一类，S18为一类，S1～S6、S8、S9、S11～S14、S16、S17、S19聚为一类；筛选出绿原酸、槲皮素-3-O-β-D-吡喃葡糖苷酸、山柰
酚-3-O-芸香糖苷以及14号峰所代表的化学成分为该药材的差异成分。19批铁线蕨药材中，绿原酸、槲皮素-3-O-β-D-吡喃葡糖苷
酸和山柰酚-3-O-芸香糖苷的平均含量分别为 0.10～4.25、0.31～7.11、0.61～12.00 mg/g。结论：本研究建立了不同产地铁线蕨药
材的HPLC-DAD指纹图谱，指认了3个共有峰；筛选出了4个影响铁线蕨药材质量的差异成分，其中绿原酸、槲皮素-3-O-β-D-吡喃
葡糖苷酸、山柰酚-3-O-芸香糖苷在不同产地铁线蕨药材中的含量差异明显。
关键词 铁线蕨；高效液相色谱-二极管阵列检测器；指纹图谱；化学计量学分析；差异成分；含量测定

Establishment and Analysis of the Fingerprints of Adiantum capillusveneris from Different Producing Areas
and Content Determination of Its Differential Components
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XIAO Dan ，FAN Dongsheng ，LIU Xing ，KONG Yi ，XUE Jinjing ，GAO Yuan（1. Guizhou Provincial Institute
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for Food Inspection，Guiyang 550004，China；2. Dept. of Pharmacy，the First Affiliated Hospital of Guizhou
University of TCM，Guiyang 550001，China）
ABSTRACT OBJECTIVE：To establish the fingerprint of Adiantum capillusveneris from different producing areas，and to
conduct chemometric analysis and content determination of differential components，so as to provide reference for quality control of
A. capillusveneris. METHODS：HPLC-DAD combined with Similarity Evaluation System of TCM Chromatogramtic Fingerprint
（2004 A edition）were used to establish fingerprint of 19 batches of A. capillusveneris from different producing areas（S1-S19）.
Common peaks were confirmed and their similarities were evaluated. Chemometric analysis methods such as cluster analysis，
principle component analysis（PCA），orthogonal partial least squares discriminant analysis（OPLS-DA）were used to evaluate the
quality of 19 batches of A. capillusveneris from different producing areas，screen the differential components，and determine the
contents of some differential components. RESULTS：Among 19 batches of A. capillusveneris from different producing areas，22
common peaks were confirmed；peak 9 was chlorogenic acid，peak 17 was quercetin-3-O-β-D-glucopyranoside，peak 20 was
kaempferol-3-O-rutoside；the similarity of 19 batches of sample were 0.677-0.962. Through cluster analysis，it was found that S7
and S10 were clustered into one category；S15 and S18 were clustered into one category；and S1-S6，S8，S9，S11-S14，S16，S17
and S19 were clustered into one category. PCA and OPLS-DA found that S7 and S10 were clustered into one category；S15 were
clustered into one category；S18 were clustered into one category；S1-S6，S8，S9，S11-S14，S16，S17 and S19 were clustered
into one category. Chlorogenic acid， quercetin-3-O-β-D-glucopyranoside， kaempferol-3-O-rutoside and chemical composition
represented by peak 14 were the differential components of the
Δ 基金项目：贵州省科技计划项目（No.黔科合支撑〔2017〕2841）；
medicinal material. Among 19 batches of A. capillusveneris，
贵阳市科技计划项目（No.筑科合同〔2019〕2-7号）
average contents of chlorogenic acid and quercetin-3-O-β-D-
＊主管检验师。研究方向：食品药品质量控制与研发。电话：
glucopyranoside and kaempferol-3-O-rutoside were 0.10-4.25，
0851-86614212。E-mail：346691298@qq.com
# 通信作者：主任药师。研究方向：食品药品质量控制与研发。 0.31-7.11， 0.61-12.00 mg/g， respectively. CONCLUSIONS：
电话：0851-86614212。E-mail：youaremygy@163.com HPLC-DAD fingerprints of A. capillusveneris from different

中国药房 2021年第32卷第17期 China Pharmacy 2021 Vol. 32 No. 17 ·2085 ·