Page 47 - 《中国药房》2021年16期

表4 10批芩连润肺汤HPLC指纹图谱共有峰的相对峰面积
Tab 4 Relative peak areas of common peaks in HPLC fingerprints of 10 batches of Qinlian runfei decoction
编号 峰1 峰2 峰3 峰4 峰5 峰6 峰7 峰8 峰9 峰10 峰11 峰12 峰13 峰14 峰15 峰16 峰17 峰18 峰19 峰20 峰21
S1 0.555 1.582 1.890 3.260 3.751 9.677 14.690 1.814 12.098 1.921 9.495 2.250 2.250 12.372 0.035 0.421 1.000 0.359 0.103 3.741 1.270
S2 0.557 1.584 1.885 3.260 3.755 9.677 14.679 1.814 12.098 1.921 9.495 2.250 2.276 12.372 0.035 0.421 1.000 0.359 0.103 3.741 1.270
S3 0.556 1.584 1.890 3.257 3.751 9.673 14.690 1.814 12.099 1.948 9.497 2.250 2.279 12.375 0.038 0.417 1.000 0.359 0.107 3.741 1.275
S4 0.557 1.583 1.886 3.260 3.750 9.677 14.658 1.808 12.098 1.985 9.486 2.251 2.275 12.379 0.040 0.416 1.000 0.359 0.116 3.740 1.278
S5 0.556 1.583 1.890 3.259 3.751 9.657 14.690 1.855 12.481 1.973 9.483 2.246 2.272 12.388 0.040 0.436 1.000 0.358 0.124 3.741 1.276
S6 0.554 1.584 1.886 3.260 3.750 9.674 14.665 1.858 12.349 1.922 9.474 2.247 2.281 12.379 0.036 0.420 1.000 0.382 0.123 3.751 1.269
S7 0.557 1.568 1.890 3.242 3.751 9.677 14.695 1.815 12.070 1.922 9.485 2.200 2.280 12.375 0.038 0.421 1.000 0.375 0.103 3.741 1.281
S8 0.556 1.583 1.890 3.260 3.737 9.675 14.690 1.844 12.075 1.918 9.473 2.186 2.277 12.349 0.035 0.427 1.000 0.366 0.103 3.740 1.270
S9 0.557 1.572 1.890 3.242 3.751 9.666 14.679 1.816 12.098 1.948 9.484 2.227 2.275 12.395 0.031 0.431 1.000 0.358 0.104 3.745 1.255
S10 0.554 1.584 1.889 3.244 3.755 9.648 14.689 1.838 12.546 1.911 9.489 2.225 2.278 12.402 0.037 0.412 1.000 0.355 0.106 3.741 1.281
RSD，％ 0.217 0.358 0.106 0.252 0.132 0.105 0.085 1.045 1.510 1.317 0.089 1.048 0.387 0.117 7.824 1.709 0. 2.417 7.672 0.097 0.600
表 5 芩连润肺汤中芦丁等 11 种成分的回归方程与线 续表6
性范围 Continued tab 6
Tab 5 Regression equations and linear ranges of 11 待测成分 已知量，mg 加入量，mg 测得量，mg 加样回收率，％ 平均加样回收率，％ RSD，％
components as rutin in Qinlian runfei decoction 鸢尾苷 0.324 2 0.342 5 0.670 3 101.05
0.299 5 0.346 4 0.641 6 98.76
待测成分 回归方程 r 线性范围，μg 野鸢尾苷 0.257 5 0.259 4 0.513 8 98.80 98.97 1.58
芦丁 Y＝52 296.71X－1 153.45 0.999 9 9.960 0～49.800 0
0.260 2 0.289 8 0.542 1 97.27
连翘酯苷A Y＝1 711 302.55X+52 945.68 0.999 8 1.974 0～9.870 0 0.260 7 0.289 2 0.552 0 100.73
连翘酯苷B Y＝2 665 728.33X+29 885.04 0.999 6 0.672 0～3.360 0 0.260 5 0.294 6 0.554 4 99.76
鸢尾苷 Y＝3 203 948.24X+7 243.38 0.999 7 0.960 0～4.800 0 0.262 2 0.294 8 0.548 0 96.95
野鸢尾苷 Y＝3 485 034.24X+1 470.96 0.999 8 0.549 0～2.745 0 0.258 4 0.269 3 0.528 3 100.22
黄芩苷 Y＝1 980 941.56X+84 596.34 0.999 9 5.040 0～25.200 0 黄芩苷 1.307 6 1.342 3 2.672 9 101.71 100.29 0.84
连翘苷 Y＝197 998.58X+15 673.14 0.999 9 1.374 0～6.870 0 1.312 9 1.342 7 2.653 1 99.81
汉黄芩苷 Y＝3 898 718.07X+10 929.51 0.999 7 0.615 0～3.075 0 1.282 0 1.342 8 2.623 6 99.91
黄芩素 Y＝3 680 141.60X－2 960.32 0.999 8 0.759 9～3.795 0 1.289 1 1.345 7 2.645 2 100.78
次野鸢尾黄素 Y＝4 028 888.92X+1 139.85 0.999 7 0.162 0～0.810 0 1.285 3 1.337 3 2.625 0 100.18
汉黄芩素 Y＝4 007 324.79X－611.99 0.999 8 0.042 0～0.210 0 1.281 0 1.347 0 2.619 2 99.35
连翘苷 0.472 5 0.474 2 0.956 1 101.98 99.02 1.64
表 6 芩连润肺汤中芦丁等 11 种成分的加样回收率试
0.471 8 0.473 8 0.934 1 97.57
验结果（n＝6） 0.474 2 0.474 3 0.940 1 98.23
Tab 6 Results of recovery tests of 11 components as 0.471 3 0.477 5 0.938 2 97.78
0.472 8 0.471 4 0.941 1 99.34
rutin in Qinlian runfei decoction（n＝6） 0.471 8 0.478 5 0.946 5 99.21
待测成分 已知量，mg 加入量，mg 测得量，mg 加样回收率，％ 平均加样回收率，％ RSD，％ 汉黄芩苷 1.686 3 1.689 1 3.401 7 101.56 99.70 1.01
芦丁 5.437 4 5.237 5 10.759 7 101.62 99.47 1.20 1.682 8 1.937 4 3.608 3 99.39
5.820 1 5.260 2 11.006 4 98.60 1.684 8 1.774 1 3.458 4 99.97
5.423 8 5.284 1 10.706 2 99.97 1.664 9 1.739 3 3.384 5 98.87
4.766 2 5.177 3 9.875 4 98.68 1.679 3 1.787 4 3.459 7 99.61
1.662 9 1.752 3 3.394 9 98.84
5.274 2 5.173 7 10.418 9 99.44
5.421 8 5.271 3 10.616 1 98.54 黄芩素 3.854 9 3.852 7 7.715 6 100.21 99.90 0.43
3.862 4 3.860 5 7.726 0 100.08
连翘酯苷A 0.226 3 0.225 3 0.455 1 101.55 98.39 1.73
0.219 3 0.228 9 0.441 9 97.25 3.842 9 3.841 6 7.648 6 99.07
3.845 6 3.850 6 7.689 5 99.83
0.213 2 0.202 0 0.412 9 98.86 3.842 9 3.842 1 7.684 6 99.99
0.253 1 0.221 3 0.467 6 96.88
3.852 8 3.851 9 7.712 5 100.20
0.199 1 0.235 1 0.428 5 97.58 次野鸢尾黄素 0.059 0 0.057 9 0.115 8 98.10 96.76 1.72
0.199 8 0.227 0 0.422 7 98.19 0.079 3 0.062 2 0.139 0 95.98
连翘酯苷B 0.342 7 0.342 1 0.678 5 98.16 95.81 1.64 0.063 3 0.062 1 0.123 2 96.46
0.344 3 0.341 8 0.666 3 94.21
0.062 7 0.062 5 0.123 2 96.80
0.343 7 0.337 4 0.667 2 95.88 0.062 8 0.062 7 0.124 9 99.04
0.327 0 0.343 6 0.660 5 97.06 0.071 8 0.072 5 0.140 1 94.21
0.345 7 0.347 0 0.672 5 94.18 汉黄芩素 0.064 4 0.062 3 0.124 7 96.79 97.43 1.47
0.339 8 0.342 8 0.666 8 95.39 0.064 3 0.064 3 0.126 5 96.73
鸢尾苷 0.342 7 0.339 3 0.686 2 101.24 100.20 1.31 0.062 9 0.065 3 0.127 9 99.54
0.341 8 0.337 3 0.673 6 98.37 0.062 3 0.060 8 0.120 8 96.22
0.391 3 0.345 8 0.741 7 101.33 0.063 9 0.065 2 0.126 8 96.40
0.294 7 0.337 8 0.634 1 100.47 0.062 1 0.077 2 0.138 4 98.83

中国药房 2021年第32卷第16期 China Pharmacy 2021 Vol. 32 No. 16 ·1961 ·