Page 82 - 2021年15期

桑根酮C对游离脂肪酸诱导人肝癌HepG2细胞脂质蓄积的改善

作用

邢菊玲 ，刘 芬 ，冯 萌 ，郝梦娇 ，黄天来 ，周欣欣 ，汤湘江 （1.广州中医药大学中药学院，广州 510006；
2
1
1
1＊
3 #
1
1
2.中山大学药学院，广州 510275；3.广东省中医院/广州中医药大学第二附属医院大学城医院神经内科，广州
510006）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2021）15-1868-06
DOI 10.6039/j.issn.1001-0408.2021.15.13
摘 要 目的：研究桑根酮C对游离脂肪酸诱导的人肝癌HepG2细胞脂质蓄积的改善作用。方法：将HepG2细胞分为对照组、模
型组、非诺贝特组（10 μmol/L）和桑根酮C低、中、高浓度组（2、4、8 μmol/L），除对照组外，其余各组细胞加入1 mmol/L游离脂肪酸
以诱导建立脂质蓄积模型，且各给药组加入相应含药培养基进行培养。采用油红O染色法观察细胞中脂质蓄积情况，并测定脂质
水平和三酰甘油（TG）含量；采用实时荧光定量聚合酶链式反应法和 Western blot 法检测细胞中过氧化物酶体增殖物激活受体α
（PPARα）、肉碱棕榈酰转移酶1（CPT-1）、成脂甾醇元件结合蛋白固醇调节元件结合蛋白1c（SREBP-1c）、脂肪酸合成酶（FAS）、沉
默信息调节因子1（SIRT1）、过氧化物酶体增殖物激活受体γ辅助激活物1α（PGC-1α）的mRNA和蛋白表达水平。结果：与对照组
比较，模型组细胞核明显萎缩、体积变小，脂滴数明显增加；细胞中脂质水平、TG含量以及SREBP-1c、FAS 的mRNA和蛋白表达水
平均显著升高（P＜0.05 或 P＜0.01），PPARα、CPT-1、SIRT1、PGC-1α 的 mRNA 和蛋白表达水平均显著降低（P＜0.01）。与模型组
比较，桑根酮C各浓度组细胞核萎缩不明显、体积大小正常，脂滴数明显减少；细胞中脂质水平、TG含量以及上述PPARα通路相关
基因的mRNA和蛋白（桑根酮C低浓度组的SREBP-1c蛋白除外）表达水平均显著逆转（P＜0.05或P＜0.01）。结论：桑根酮C可改
善HepG2细胞的脂质蓄积；其作用机制可能与调节PPARα信号通路、提高细胞脂质氧化能力、抑制脂质合成有关。
关键词 桑根酮C；HepG2细胞；脂质蓄积；PPARα信号通路
Improvement Effects of Sanggenone C on Lipid Accumulation in FFA-induced Human Liver Cancer
HepG2 Cells
XING Juling ，LIU Fen ，FENG Meng ，HAO Mengjiao ，HUANG Tianlai ，ZHOU Xinxin ，TANG Xiangjiang 3
1
1
1
1
2
1
（1. School of TCM，Guangzhou University of TCM，Guangzhou 510006，China；2. School of Pharmacy，Sun
Yat⁃sen University，Guangzhou 510275，China；3. Dept. of Neurology，Guangdong Provincial Hospital of TCM/
University Town Hospital，the Second Affiliated Hospital of Guangzhou University of TCM，Guangzhou
510006，China）
ABSTRACT OBJECTIVE：To study the improvement effects of sanggenone C on lipid accumulation in human liver cancer
HepG2 cells induced by free fatty acid （FFA）. METHODS：HepG2 cells were divided into control group，model group，
fenofibrate group（10 μmol/L），sangerone C low，medium and high concentration groups（2，4，8 μmol/L）. Except for control
group，other groups were treated with 1 mmol/L FFA to induce lipid accumulation model，and administration groups were cultured
with relevant medium containing drugs. The lipid accumulation was observed by oil red O staining，and lipid level and triglyceride
（TG） content were also determined. Real-time PCR and Western blot assay were adopted to detect the mRNA and protein
expression of PPARα，CPT-1，SREBP-1c，FAS，SIRT1 and PGC-1α in HepG2 cells. RESULTS：Compared with control group，
the nucleus was atrophied significantly and the volume became smaller，and the number of lipid droplets was significantly
increased；the level of lipid，TG content，mRNA and protein expression of SREBP-1c and FAS were significantly increased（P＜
0.05 or P＜0.01），mRNA and protein expression of PPARα，CPT-1，SIRT1 and PGC-1α were decreased significantly（P＜0.01）.
Compared with model group，no obvious nucleus atrophy and normal volume were observed in sangerone C groups，and the
number of lipid droplets was significantly reduced；the levels of lipid，TG content，mRNA and protein expression of PPAR α
pathway related genes（except for SREBP-1c protein in saggenone C low concentration group）were significantly reversed（P＜
0.05 or P＜0.01）. CONCLUSIONS：Sangenone C can significantly improve the lipid accumulation of HepG2 cells，and its
mechanism may associated with regulating PPAR α signaling
＊ 硕 士 研 究 生 。 研 究 方 向 ：中 药 内 分 泌 药 理 学 。 E-mail： pathway，improving cell lipid oxidation ability and inhibiting
15562591483@163.com lipid synthesis.
# 通信作者：副教授，硕士生导师。研究方向：中药新药与开发。 KEYWORDS Sanggenone C；HepG2 cells；Lipid accumu-
E-mail：yanchengys@163.com lation；PPARα signaling pathway

·1868 · China Pharmacy 2021 Vol. 32 No. 15 中国药房 2021年第32卷第15期