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黄连花薹 HPLC 指纹图谱的建立及其抗氧化和抑菌作用谱效关

系研究 Δ

杨 慧 ，张 佳 ，刘博文 ，刘 钱 ，李松洋 ，吴 磊 ，徐玉玲 ，刘 涛（1.成都大学食品与生物工程学院，成
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都 610106；2.成都大学四川抗菌素工业研究所，成都 610106；3.成都大学张澜学院，成都 610106）
中图分类号 R284.1；R285.5 文献标志码 A 文章编号 1001-0408（2021）05-0559-06
DOI 10.6039/j.issn.1001-0408.2021.05.09
摘 要 目的：建立黄连花薹的高效液相色谱（HPLC）指纹图谱，并研究其与抗氧化和抑菌作用的谱效关系。方法：以14批不同
产地的黄连花薹为对象，采用HPLC法测定。色谱柱为Supersil C18，流动相为乙腈-0.1％磷酸溶液（梯度洗脱），流速为1.0 mL/min，
柱温为25 ℃，检测波长为329 nm，进样量为10 μL。采用《中药指纹图谱相似度评价系统》（2012A版）建立14批黄连花薹的指纹
图谱并进行相似度评价和共有峰指认。以DPPH自由基清除率和羟自由基清除率为抗氧化作用指标，以相对抑菌活性（大肠埃希
菌）为抑菌作用指标，利用SPSS 21.0软件对黄连花薹共有峰与上述两种药效指标进行Pearson相关性分析，建立谱效关系模型，并
进行验证。结果：黄连花薹的HPLC指纹图谱中共得到7个共有峰，相似度均不低于0.916，并指认5号峰为盐酸小檗碱。7个共有
峰均与DPPH自由基清除率呈正相关；1～3、4、6、7号峰与羟自由基清除率呈正相关，5号峰与羟自由基清除率呈负相关；5号峰与
相对抑菌活性呈正相关。经验证，黄连花薹样品的DPPH自由基清除率、羟自由基清除率、相对抑菌活性的预测值与测量值的相
对误差为0.92％～14.5％。结论：所建立的谱效关系模型可用于黄连花薹抗氧化、抑菌作用的评价；1、2、3、4、6、7号峰所代表化学
成分是黄连花薹抗氧化作用的物质基础，盐酸小檗碱是其抑菌作用的物质基础。
关键词 黄连花薹；高效液相色谱；指纹图谱；抗氧化作用；抑菌作用；物质基础
Establishment of HPLC Fingerprint of Coptis chinensis Inflorescence and Its Spectrum-effect Relationship
with Antioxidant and Antibacterial Effects
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YANG Hui ，ZHANG Jia ，LIU Bowen ，LIU Qian ，LI Songyang ，WU Lei ，XU Yuling ，LIU Tao（1. School of
Food and Bioengineering，Chengdu University，Chengdu 610106，China；2. Sichuan Institute of Antibiotics
Industry，Chengdu University，Chengdu 610106，China；3. Zhanglan College，Chengdu University，Chengdu
610106，China）
ABSTRACT OBJECTIVE：To establish HPLC fingerprint of Coptis chinensis inflorescence，and study its spectrum-effect
relationship with antioxidant and antibacterial effects. METHODS：Taking 14 batches of C. chinensis inflorescence from different
producing areas as the object，HPLC method was adopted. The determination was performed on Supersil C18 column with mobile
phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The column
temperature was set at 25 ℃. The detection wavelength was set at 329 nm，and sample size was 10 μL. The fingerprints of 14
batches of C. chinensis inflorescence were established by Similarity Evaluation System of TCM Fingerprint（2012 A edition），and
the similarity evaluation and common peak identification were carried out. Taking DPPH free radical scavenging rate and hydroxyl
radical scavenging rate as antioxidant effects index，relative antibacterial activity（Escherichia coli）as antibacterial effect index，
SPSS 21.0 software was adopted to analyze the Pearson correlation between common peaks of C. chinensis inflorescence and above
efficacy indexes；their spectrum-effect relationship was established and validated. RESULTS：A total of 7 common peaks were
obtained in HPLC fingerprint of C. chinensis inflorescence，and the similarity was no less than 0.916. No. 5 peak was identified as
berberine hydrochloride. Seven common peaks were positively correlated with DPPH free radical scavenging rate；No. 1-3，4，6，7
peaks were positively correlated with hydroxyl radical scavenging rate，while No. 5 peak was negatively correlated with hydroxyl
radical scavenging rate. There was a positive correlation between No. 5 peak and antibacterial activity in vitro. After validation，
relative error between the predicted values and the measured
Δ 基金项目：国家重点研发计划中医药现代化研究重点专项
values of DPPH free radical scavenging rate，hydroxyl radical
（No.2017YFC1701902）
scavenging rate and relative antibacterial activity was 0.92％-
＊本科生。研究方向：中成药再评价及中药资源综合利用。电
14.5％ . CONLUSIONS： The established spectrum-effect
话：028-64616063。E-mail：2316359352@qq.com
# 通信作者：副教授，硕士生导师。研究方向：中成药新药及质量 relationship model can be used to evaluate antioxidant and
再评价。电话：028-61302236。E-mail：xuyuling@cdu.edu.cn antibacterial effects of C. chinensis inflorescence. The chemical

中国药房 2021年第32卷第5期 China Pharmacy 2021 Vol. 32 No. 5 ·559 ·