Page 113 - 《中国药房》2021年2期

滨蒿内酯对四氯化碳致小鼠急性肝损伤的保护作用研究 Δ

彭 静 ，陈 曦（1.乐山职业技术学院中医教研室，四川 乐山 614000；2.乐山市市中区人民医院中医科，四
1＊
2
川 乐山 614000）

中图分类号 R965 文献标志码 A 文章编号 1001-0408（2021）02-0231-05
DOI 10.6039/j.issn.1001-0408.2021.02.18

摘 要 目的：研究滨蒿内酯对四氯化碳（CCl4 ）致小鼠急性肝损伤的保护作用及潜在分子机制。方法：将50只雄性昆明种小鼠随
机分为正常对照组、模型组、水飞蓟素组（阳性对照，120 mg/kg）和滨蒿内酯高、低剂量组（60、30 mg/kg），每组10只。各给药组小
鼠均灌胃相应药物，正常对照组和模型组小鼠灌胃等体积0.5％羧甲基纤维素钠溶液，每日1次，连续给药7 d。末次给药结束2 h
后，除正常对照组小鼠腹腔注射等体积橄榄油外，其余组小鼠均一次性腹腔注射0.1％CCl4橄榄油溶液（1 mL/100 g）以建立急性肝
损伤模型。采用苏木精-伊红（HE）染色法观察小鼠肝组织病理性改变；采用酶联免疫吸附法检测小鼠血清中天冬氨酸转氨酶
（AST）、丙氨酸转氨酶（ALT）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的活性和白细胞介素 1β（IL-1β）、IL-6、肿瘤坏死因子α
（TNF-α）、丙二醛（MDA）的含量；采用蛋白免疫印迹法检测小鼠肝组织中核因子κB（NF-κB）通路相关蛋白[NF-κB p65、核因子κ
Bα激酶抑制蛋白（IκBα）]的磷酸化水平。结果：与正常对照组比较，模型组小鼠血清中AST、ALT活性和MDA、IL-1β、IL-6、TNF-α
含量均显著升高，SOD、CAT活性均显著降低（P＜0.05）；肝组织出现明显的病理性改变，且肝组织中NF-κB p65和IκBα蛋白的磷酸化
水平均显著升高（P＜0.05）。与模型组比较，水飞蓟素组和滨蒿内酯高、低剂量组小鼠血清中相关因子活性或含量水平均显著逆转
（P＜0.05）；肝组织病理性改变程度明显减轻，且肝组织中NF-κB p65和IκBα蛋白的磷酸化水平均显著降低（P＜0.05）。结论：滨蒿内
酯对CCl4所致小鼠急性肝损伤具有保护作用，该作用与降低氧化应激水平以及阻断NF-κB通路的活化、进而抑制炎症反应有关。
关键词 滨蒿内酯；急性肝损伤；NF-κB通路；氧化应激；炎症；小鼠

Study on Protective Effects of Scoparone on Acute Liver Injury Induced by CCl4 in Mice
2
PENG Jing ，CHEN Xi（1. Teaching and Research Section of Traditional Chinese Medicine，Leshan Vocational
1
and Technical College，Sichuan Leshan 614000，China；2. Dept. of Traditional Chinese Medicine，Leshan
Shizhong District People’s Hospital，Sichuan Leshan 614000，China）

ABSTRACT OBJECTIVE：To study the protective effects of scoparone on acute liver injury induced by CCl4 in mice and its
potential molecular mechanism. METHODS：Fifty male Kunming mice were randomly divided into normal control group，model
group，silymarin group（positive control，120 mg/kg），scoparone high-dose and low-dose groups（60，30 mg/kg），with 10 mice
in each group. Administration groups were given relevant medicine intragastrically. Normal control group and model group were
given constant volume of 0.5％ sodium carboxymethyl cellulose solution，once a day，for 7 days. Two hours after last medication，
except normal control group was intraperitoneally injected constant volume of olive oil，other groups were intraperitoneally injected
0.1％ CCl4 olive oil solution（10 mL/kg）at one time to establish the acute liver injury model. The pathological changes of liver
tissues in mice were observed by HE staining；the activity of AST，ALT，SOD and CAT and the contents of IL-1β，IL-6，TNF-α
and MDA in serum were measured by ELISA；the phosphorylation of nuclear factor κB（NF-κB）pathway related proteins（NF-κB
p65，I κ B α）in liver tissue were detected by Western blotting assay. RESULTS：Compared with normal control group，serum
activities of AST and ALT，the contents of MDA，IL-1 β ，IL-6 and TNF-α were significantly increased in model group，the
activities of SOD and CAT were decreased significantly（P＜0.05）；obvious pathological changes were observed in liver tissues；
phosphorylation levels of NF-κB p65 and IκBα protein in liver tissues were significantly increased（P＜0.05）. Compared with
model group，the activities or contens of related factors in serum of mice were significantly reversed in silymarin group and
scoparone high-dose and low-dose groups （P＜0.05）；the pathological changes of liver tissues were significantly reduced；the
phosphorylation levels of NF-κB p65 and IκBα protein in liver tissues were significantly reduced（P＜0.05）. CONCLUSIONS：
Scoparone has a protective effect on CCl4-induced acute liver injury in mice，which is related to reducing oxidative stress levels and
blocking the activation of NF-κB pathway，thereby inhibiting inflammatory response.
KEYWORDS Scoparone；Acute liver injury；NF-κB pathway；Oxidative stress；Inflammation；Mice

滨 蒿 内 酯 为 传 统 中 药 滨 蒿 Artemisia scoparia
Δ 基金项目：乐山市重点科技计划项目（No.19SZD207）
＊副教授，硕士。研究方向：中医药治疗慢性病。电话：0833- Waldst. et Kit.、茵陈蒿 Artemisia capillaries Thunb.和石
2152787。E-mail：pengjingls@126.com 斛 Dendrobium Sw.中的有效成分，又名七叶内酯二甲

中国药房 2021年第32卷第2期 China Pharmacy 2021 Vol. 32 No. 2 ·231 ·