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补阳还五汤对高脂血症模型大鼠脂质代谢及肝组织的影响 Δ

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张玉昆 ，袁 茵，冯月男，卞敬琦，肖洪彬 ，牛雯颖（黑龙江中医药大学实验实训中心，哈尔滨 150040）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2020）10-1203-05
DOI 10.6039/j.issn.1001-0408.2020.10.09

摘 要 目的：探讨补阳还五汤对高脂血症模型大鼠脂质代谢及肝组织的影响。方法：将雄性SD大鼠随机分为空白对照组、模型
组、阳性对照组（辛伐他汀0.004 g/kg）和补阳还五汤低、高剂量组（3.5、14.0 g/kg，按生药总量计），每组10只。除空白对照组大鼠
灌胃水外，其余各组大鼠均按体质量灌胃脂肪乳剂复制高脂血症模型。从造模第21天开始，各组大鼠上午继续灌胃水或脂肪乳
剂，下午灌胃水或相应药物，连续20 d。末次给药后，检测各组大鼠血浆中三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇
（LDL-C）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、卵磷脂胆固醇脂酰基转移酶（LCAT）、磷脂转移蛋白（PLTP）、肝脂酶
（HL）含量以及肝细胞中脂蛋白脂肪酶（LPL）、微粒体三酰甘油转运蛋白（MTP）、3-羟基-3-甲基戊二酸单酰辅酶A（HMG-CoA）还
原酶的含量，并观察各组大鼠肝组织的病理形态学变化。结果：与空白对照组比较，模型组大鼠TG、TC、LDL-C、ALT、HMG-CoA
还原酶含量均显著升高（P＜0.05 或 P＜0.01），HL、LPL、MTP 含量均显著降低（P＜0.01）；与模型组比较，各给药组大鼠 TG、TC、
LDL-C、HMG-CoA还原酶含量以及补阳还五汤低、高剂量组大鼠ALT、AST含量均显著降低（P＜0.05或P＜0.01），各给药组大鼠
HL、MTP含量以及阳性对照组大鼠LPL含量均显著升高（P＜0.05或P＜0.01）。病理切片显示，模型组大鼠肝细胞排列不规则，可
见明显的细胞脂肪变性；阳性对照组和补阳还五汤高剂量组大鼠肝细胞较空白对照组几乎无异常变化，补阳还五汤低剂量组大鼠
肝组织有轻微细胞脂肪变性。结论：补阳还五汤对高脂血症模型大鼠的降脂作用显著，这作用可能与该方通过调控脂质代谢相关
酶及转运蛋白的表达而调节脂质的吸收、转运、代谢有关。
关键词 高脂血症；大鼠；补阳还五汤；脂质代谢；肝组织

Effects of Buyang Huanwu Decoction on Lipid Metabolism and Hepatic Tissue in Hyperlipemia Model Rats
ZHANG Yukun，YUAN Yin，FENG Yuenan，BIAN Jingqi，XIAO Hongbin，NIU Wenying（Experimental and
Training Centre，Heilongjiang University of TCM，Harbin 150040，China）

ABSTRACT OBJECTIVE：To investigate the effects of Buyang huanwu decoction on lipid metabolism and hepatic tissue in
hyperlipemia model rats. METHODS：Male SD rats were randomly divided into blank control group，model group，positive
control group （simvastatin 0.004 g/kg），Buyang huanwu decoction low-dose and high-dose groups （3.5，14.0 g/kg，by crude
drugs），with 10 rats in each group. Except that blank control group was given water intragastrically，hyperlipidemia mode of other
groups was established by intragastric administration of fat emulsion according to the body weight. From the 21st day after
modeling，the rats in each group were given water or fat emulsion in the morning and water or corresponding drugs in the
afternoon for consecutive 20 days. After last medication，the contents of TG，TC，LDL-C，ALT，AST，LCAT，PLTP and HL in
plasma were determined in each group；the contents of LPL，MTP and HMG-CoA redutase in liver tissue were also determined.
The pathological changes of liver tissue were observed in each group. RESULTS：Compared with blank control group，the contents
of TG，TC，LDL-C，ALT and HMG-CoA reductase were increased significantly in model group（P＜0.05 or P＜0.01），while the
contents of HL，LPL and MTP were decreased significantly（P＜0.01）. Compared with model group，the contents of TG，TC，
LDL-C and HMG-CoA reductase in administration groups as well as the contents of ALT，AST in Buyang huanwu decoction
low-dose and high-dose groups were decreased significantly（P＜0.05 or P＜0.01）；the contents of HL and MTP in administration
groups as well as the content of LPL in positive control group were increased significantly（P＜0.05 or P＜0.01）. Pathological
sections showed that the hepatocytes of model group were arranged irregularly，and there was obvious cell steatosis. The
hepatocytes in positive control group and Buyang Huanwu decoction high-dose group had almost no abnormal change compared
with blank control group，and there was a small amount of
Δ 基金项目：国家自然科学基金资助项目（No.81703981）；黑龙江
slight cell steatosis in Buyang Huanwu decoction low-dose
中医药大学科研基金项目（No.201739）
＊实验师，博士研究生。研究方向：方剂学配伍规律与药学基 group. CONCLUSIONS： Buyang huanwu decoction has a
significant lipid-lowering effect on hyperlipemia model rats，
础。E-mail：zhang520-888@163.com
# 通信作者a：教授，博士生导师，硕士。研究方向：方剂配伍规律 which may be related to the regulation of lipid absorption，
与药效物质基础。电话：0451-82193425。E-mail：hrbxiaohongbin@ transport and metabolism by regulating the expression of lipid
126.com metabolism related enzymes and transporter.
# 通信作者b：研究员，博士。研究方向：方剂配伍规律与药学基 KEYWORDS Hyperlipemia； Rat； Buyang huanwu
础。电话：0451-82193492。E-mail：121505982@qq.com decoction；Lipid metabolism；Hepatic tissue

中国药房 2020年第31卷第10期 China Pharmacy 2020 Vol. 31 No. 10 ·1203 ·