Page 43 - 2020年1月第31卷第2期

Study on the Mechanism of Tibetan Medicine Wuwei Shexiang Pills in the Treatment of“Grum bu”
Disease Based on“Ro Nus ZhurJes-Network Target-Molecular Docking”
Wenchengdangzhi ，ZHANG Yunsen ，Renzhenwangjia ，Cairangnanjia 1， 2 ，Gongbaodongzhi ，Qienixiangmao ，
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Cairangji ，Ganghuanchenlei ，ZHANG Yi（1. Ethnic Medicine School，Chengdu University of TCM，Chengdu
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611137，China；2. Graduate School，Tibet University of Tibetan Medicine，Lhasa 850000，China；3. Research
Center for Academic Inheritance and Innovation of Ethnic Medicine，Chengdu University of TCM，Chengdu
611137，China）
ABSTRACT OBJECTIVE：To investigate the potential mechanism of Tibetan medicine Wuwei shexiang pills in the treatment of
“Grum bu”disease （i.e. rheumatoid arthritis）. METHODS：Referring to Sibu Yidian，Drug Standard of the Ministry of Public
Health （Tibetan Medicine），Jingzhubencao，the constituent，dose and flavor of Wuwei shexiang pills were collected；vector
structure model was built. From the aspects of six tastes，three tastes after digestion and seventeen effects，the properties of the
formulation were analyzed； the “formulation-drug property-disease” network for the treatment of “Grum bu” disease was
constructed by using Gephi 0.9.2 complex network software. The effective components of Wuwei shexiang pills were screened by
using the pharmacology analysis platform of TCM system and the database of organic small molecule biological activity. The target
proteins corresponding to the active components were predicted by using BATMAN-TCM network pharmacology research platform；
the target proteins of“Grum bu”disease were searched by ETCM database. On the basis of screening the common targets of the
two，David 6.8 bioinformatics resource database was adopted to conduct gene ontology （GO） analysis and KEGG pathway
enrichment analysis；the network of Wuwei shexiang pills-“Grum bu”- target-pathway was constructed by using Cytoscape 3.7.0
software，and the network topology analysis was carried out to screen the core targets. Using Glide score as evaluation index，
Maestro Version 11.1.011 software was used for molecular docking of above core targets with effective components of Wuwei
shexiang pills. RESULTS & CONCLUSIONS：Wuwei shexiang pills contains myrobalan，Aconitum pendulum，Aucklandia lappa，
Acorus calamus and artificial moschus，and the highest dosage was myrobalan. The six tastes of this formulation were mainly bitter
and sweet；the three tastes after digestion were mainly bitter；the seventeen effects were mainly blunt，cool and heavy，which were
mainly used to treat twenty characteristics such as acute，hot and light. In“formulation-property-disease”network，higher values of
edge weight between seventeen effects and twenty characteristics were cooling effect-heat， blunt effect-sharpness， heavy
effect-light，dilute effect-odor（edge weight values≥430），etc. A total of 2 306 potential targets of effective components of Wuwei
shexiang pills，211 targets related to“Grum bu”disease，32 common targets and 29 corresponding effective components were
obtained. The results of GO analysis showed that 52 relative results were predicted，the common targets mainly located in the
extracellular area，nucleus and other parts，mainly including biological processes and molecular functions such as immune
response，inflammatory response，cytokine activity. The results of KEGG enrichment were significant in 31 pathways（P＜0.05），
involving TNF signaling pathway，cancer pathway and other signaling pathways. There were 94 active components，targets and
pathway nodes in the network of Wuwei shexiang pills-“Grum bu”-target-pathway，and 460 edges；TNF，FAS，IL6，IL10，
FASLG，PTGS2 and IL1B were the core targets of the network， connected with effective components such as quinic acid，
thymol，dehydroepiandrosterone，norcaxone by Van Der Waals force，hydrogen bond and hydrophobic interaction，Pi-cation bond
and other bonds，so as to play a role in the treatment of“Grum bu”disease.
KEYWORDS Tibetan medicine； Wuwei shexiang pills； Grum bu； Rheumatoid arthritis； Ro Nus ZhurJes； Network
pharmacology；Molecular docking

类 风 湿 性 关 节 炎（RA）在 藏 医 中 称 为“ 真 布 ” 可引起多器官、多系统疾病等特点 。藏药五味麝香丸
[2]
（藏语： ）。藏医治疗“真布”病具有悠久的历史和显 （藏语： ）始载于《四部医典》，现收载于 2015 年版
著的疗效 。藏医认为，“真布”是由黄水病变引起，其病 《中国药典》（一部） ，由诃子（去核）、木香、人工麝香、铁
[3]
[1]
理机制为三胃火失衡、糟粕入肝，进而影响变色赤巴功 棒锤（又名黑草乌）、藏菖蒲等 5 味药材组合而成，主治
能，使未完全分离的血液进入胆囊，导致黄水遍于全身， “森”（意为微生物）引起的热症，如痤疮、疫热、疼痛、咽
并在关节淤积，诱发“真布”病（发病机制见图 1）；此外， 峡炎等，尤其对黄水所致疾病具有良好疗效，但目前关
疫热、劳累、宁尼（意为白天嗜睡）等亦可引起赤巴热遍 于该方治疗“真布”病的药性及作用机制尚缺乏深入研
布全身，是“真布”病的另一诱因 。“真布”病在我国患病 究。为此，本课题组构建了“味性化味-网络靶点-分子对
[2]
率很高，且持续处于较高的发病水平，具有致残率高和 接”三位一体的研究模式，拟从“方剂-药物-靶标-通路”

中国药房 2020年第31卷第2期 China Pharmacy 2020 Vol. 31 No. 2 ·165 ·