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丹皮酚调控NF-κB/HIF-1α信号通路抑制膀胱癌T24细胞迁移的

作用及机制
Δ

#
艾欣瑶 ，陈雯佳，陈 曦，王颖峥，王英豪，黄美霞（福建中医药大学药学院，福州 350122）
＊
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2025）15-1871-05
DOI 10.6039/j.issn.1001-0408.2025.15.09

摘 要 目的 探讨丹皮酚调控核因子κB（NF-κB）/缺氧诱导因子1α（HIF-1α）信号通路介导的有氧糖酵解从而抑制膀胱癌T24细
胞迁移的作用及机制。方法 将 T24 细胞分为对照组、顺铂组（阳性对照，3.001 μg/mL）和丹皮酚低、中、高剂量组（100、200、
400 μg/mL），给药干预24 h后，检测丹皮酚对T24细胞迁移能力的影响（以细胞划痕愈合率表示），检测丹皮酚对T24细胞线粒体
膜电位的影响（以红绿荧光强度比值表示），检测T24细胞中腺苷三磷酸（ATP）浓度和乳酸含量，检测细胞中NF-κB/HIF-1α信号通
路、迁移相关蛋白以及有氧糖酵解关键酶的表达水平。结果 与对照组比较，丹皮酚中、高剂量组和顺铂组细胞划痕愈合率均显著
降低（P＜0.01）；丹皮酚各剂量组细胞中 NF-κB/HIF-1α 信号通路相关蛋白 NF-κB、HIF-1α，迁移相关蛋白基质金属蛋白酶 2
（MMP2）、MMP9、血管内皮生长因子，有氧糖酵解关键酶葡萄糖转运蛋白1、己糖激酶2、丙酮酸激酶M2表达水平均有不同程度降
低，大部分差异有统计学意义（P＜0.05或P＜0.01）；丹皮酚中、高剂量组细胞线粒体红绿荧光强度比值均显著降低（P＜0.01）；丹
皮酚高剂量组细胞 ATP 浓度以及丹皮酚各剂量组细胞乳酸含量均显著降低（P＜0.05 或 P＜0.01）。结论 丹皮酚可抑制膀胱癌
T24细胞的迁移，其作用机制可能与抑制NF-κB/HIF-1α信号通路、下调有氧糖酵解关键酶活性有关。
关键词 丹皮酚；膀胱癌；NF-κB/HIF-1α信号通路；有氧糖酵解；迁移

Effect and mechanism of paeonol in regulating NF-κB/HIF-1α signaling pathway to inhibit the migration
of bladder cancer T24 cells
AI Xinyao，CHEN Wenjia，CHEN Xi，WANG Yingzheng，WANG Yinghao，HUANG Meixia（School of
Pharmacy， Fujian University of Traditional Chinese Medicine， Fuzhou 350122， China）

ABSTRACT OBJECTIVE To investigate the role and mechanism of paeonol in inhibiting the migration of bladder cancer T24
cells by regulating nuclear factor κB （NF-κB）/hypoxia-inducible factor-1α （HIF-1α）-mediated aerobic glycolysis. METHODS T24
cells were divided into control group， cisplatin group （positive control， 3.001 μg/mL）， and paeonol low-， medium- and high-dose
groups （100， 200， 400 μg/mL）， respectively. After 24 h of administration intervention， the effect of paeonol on the migration
ability of T24 cells was detected （expressed by the cell scratch wound healing rate）. The effect of paeonol on the mitochondrial
membrane potential of T24 cells was detected （expressed by the ratio of red/green fluorescence intensity）. Cellular adenosine
triphosphate （ATP） levels and lactate content in T24 cells were measured. The levels of NF-κB/HIF-1α signaling pathway， the
expression of migration-related proteins， and key enzymes involved in aerobic glycolysis in the cells were all determined.
RESULTS Compared with the control group， the cell scratch wound healing rates in the paeonol medium- and high-dose groups
and the cisplatin group were decreased significantly （P＜0.01）； in the paeonol groups， the expression levels of NF-κB/HIF-1α
signaling pathway-related proteins such as NF- κB and HIF-1α， migration-related proteins such as matrix metalloproteinase 2
（MMP2）， MMP9， and vascular endothelial growth factor， as well as key enzymes involved in aerobic glycolysis such as glucose
transporter 1， hexokinase 2 and pyruvate kinase isozyme type M2， were all reduced to varying degrees in the cells， most of these
reductions showed statistically significant differences （P＜0.05 or P＜0.01）； the ratio of red/green fluorescence intensity in
mitochondria of cells in the medium- and high-dose paeonol groups were significantly decreased （P＜0.01）； the ATP concentration
in cells of the paeonol high-dose group， and the lactate content
Δ 基金项目 国家中医药管理局临床中药学高水平中医药重点学 in cells across all paeonol groups were significantly decreased
科建设项目（No.zyyzdxk-2023107）；福建省自然科学基金项目（No. （P＜0.05 or P＜0.01）. CONCLUSIONS Paeonol significantly
2023J01857）；福 建 中 医 药 大 学 基 础 类 学 科 科 研 提 升 计 划（No. inhibits the migration of bladder cancer T24 cells， and its
XJC2022013）；福建中医药大学省级财政资助专项（No.X2023005-财 mechanism of action may be related to the inhibition of the
政专项） NF-κB/HIF-1α signaling pathway， and the down-regulation of
＊第一作者 硕士研究生。研究方向：中药药理与临床。E-mail：
key enzyme activities involved in aerobic glycolysis.
1791876695@qq.com
# 通信作者 副教授，硕士生导师。研究方向：中药药理与临床。 KEYWORDS paeonol； bladder cancer； NF-κB/HIF-1α sig-
E-mail：2008009@fjtcm.edu.cn naling pathway； aerobic glycolysis； migration

中国药房 2025年第36卷第15期 China Pharmacy 2025 Vol. 36 No. 15 · 1871 ·