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·药学研究·

香砂益胃汤对慢性萎缩性胃炎大鼠胃黏膜损伤的改善作用及机制 Δ

＊
夏鹏飞 ，金 迪，梁 金，余 意，杜进军，金战勇，方 俊，杨 霞，刘惠武 [武汉市中医医院脾胃肝胆病科（消
#
化内科），武汉 430050]

中图分类号 R965 文献标志码 A 文章编号 1001-0408（2025）11-1311-06
DOI 10.6039/j.issn.1001-0408.2025.11.04

摘 要 目的 探讨香砂益胃汤对慢性萎缩性胃炎（CAG）大鼠胃黏膜损伤的改善作用及机制。方法 将大鼠随机分为正常对照
组，模型组，香砂益胃汤低、中、高剂量组（6、12、18 g/kg，以生药量计），香砂益胃汤高剂量+740 Y-P组[香砂益胃汤18 g/kg+转化生
长因子β1 （TGF-β1 ）/磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）通路激活剂740 Y-P 10 mg/kg]，每组18只。各组大鼠灌胃/注射相
应药物，每天1次，连续4周。检测大鼠胃黏膜血流量、血清中胃激素[胃动素（MTL）、胃泌素（GAS）、胃蛋白酶（PP）]和炎症因子
[肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6]水平；观察大鼠胃黏膜组织病理形态；检测大鼠胃黏膜细胞凋亡率；检测
大鼠胃黏膜组织中TGF-β1/PI3K/Akt信号通路相关蛋白和凋亡相关蛋白[B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）]的表达
水平。结果 与正常对照组比较，模型组大鼠胃黏膜组织结构异常，胃黏膜上皮细胞脱落，炎症细胞浸润明显；胃黏膜血流量，血清
中MTL、GAS、PP水平，胃黏膜组织中Bcl-2蛋白表达水平均显著降低（P＜0.05）；血清中TNF-α、IL-1β、IL-6水平，细胞凋亡率，胃
黏膜组织中Bax、TGF-β1蛋白表达水平和PI3K、Akt蛋白磷酸化水平均显著升高（P＜0.05）。与模型组比较，香砂益胃汤各剂量组
大鼠胃黏膜组织病理损伤减轻，炎症细胞浸润减少，上述定量指标均显著改善（P＜0.05）。与香砂益胃汤高剂量组比较，香砂益胃
汤高剂量+740 Y-P组大鼠胃黏膜病理损伤明显加重，上述定量指标均显著逆转（P＜0.05）。结论 香砂益胃汤可减轻CAG大鼠胃
黏膜损伤，其作用机制与抑制TGF-β1/PI3K/Akt信号通路相关。
关键词 香砂益胃汤；慢性萎缩性胃炎；胃黏膜损伤；TGF-β1/PI3K/Akt信号通路；机制

Improvement effects and mechanism of Xiangsha yiwei tang on gastric mucosal injury in rats with chronic
atrophic gastritis
XIA Pengfei，JIN Di，LIANG Jin，YU Yi，DU Jinjun，JIN Zhanyong，FANG Jun，YANG Xia，LIU Huiwu[Dept. of
Spleen， Stomach， Hepatobiliary Diseases （Dept. of Gastroenterology）， Wuhan Hospital of Traditional Chinese
Medicine， Wuhan 430050， China]

ABSTRACT OBJECTIVE To investigate the improvement effects and mechanism of Xiangsha yiwei tang on gastric mucosal
injury in rats with chronic atrophic gastritis （CAG）. METHODS Rats were randomly assigned into normal control group， model
group， Xiangsha yiwei tang low-， medium- and high-dose groups （6， 12， 18 g/kg， calculated by crude drug）， and high-dose
group of Xiangsha yiwei tang+740 Y-P [Xiangsha yiwei tang 18 g/kg+transforming growth factor β1/phosphatidyl inositol 3 kinase/
protein kinase B（TGF-β1/PI3K/Akt） pathway activator group 740 Y-P 10 mg/kg]， with 18 rats in each group. Rats in each group
were administered the corresponding drugs via oral gavage or injection， once daily， for 4 consecutive weeks. Gastric mucosal blood
flow， the levels of serum gastrointestinal hormones [including motilin （MTL）， gastrin （GAS）， and pepsinogen （PP）]， as well as
inflammatory cytokines [including tumor necrosis factor- α （TNF- α）， interleukin-1β （IL-1β）， IL-6] in rats were measured.
Pathological damage to gastric mucosal tissue was observed in rats； the apoptotic rate of gastric mucosal cells was detected. The
expressions of TGF- β1/PI3K/Akt signaling pathway-related proteins and apoptosis-related proteins [including B-cell lymphoma-2
（Bcl-2） and Bcl-2-associated X protein （Bax）] in the gastric mucosal tissues of rats were assessed. RESULTS Compared with
normal control group， model group had abnormal gastric mucosal tissue structure， with shedding of gastric mucosal epithelial cells，
and prominent infiltration of inflammatory cells. Gastric mucosal blood flow， the serum levels of MTL， GAS， PP， and Bcl-2
protein expression were lowered significantly， while serum levels of TNF-α， IL-1β and IL-6， apoptosis rate， protein expressions of
Bax and TGF-β1， the phosphorylations of PI3K and Akt were
Δ 基金项目 武汉市中医药科研项目（No.WZ24B90） increased significantly （P＜0.05）. Compared with model
＊第一作者 副主任医师。研究方向：中医脾胃病。电话：027-
group， Xiangsha yiwei decoction groups exhibited attenuated
84516759。E-mail：jmdcp4@163.com
# 通信作者 主任医师。研究方向：中医肝胆病、脾胃病。E-mail： histopathological injuries in gastric mucosal tissues， reduced
whlhw@126.com inflammatory cell infiltration， and significant improvements in

中国药房 2025年第36卷第11期 China Pharmacy 2025 Vol. 36 No. 11 · 1311 ·