Page 52 - 《中国药房》2025年6期

复方芩柏颗粒对溃疡性结肠炎大鼠的改善作用及机制研究
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贺守炎 ，罗雯鹏 ，潘 燎 ，肖金银 ，王真权 （1. 湖南中医药大学第二附属医院肛肠三科，长沙 410005；
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2.湖南中医药大学第二附属医院肛肠二科，长沙 410005；3.湖南中医药大学研究生院，长沙 410036）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2025）06-0686-06
DOI 10.6039/j.issn.1001-0408.2025.06.08

摘 要 目的 基于短链脂肪酸（SCFA）及其作用靶点G蛋白偶联受体（GPR），探究复方芩柏颗粒对溃疡性结肠炎（UC）大鼠的改
善作用及潜在机制。方法 将雄性SD大鼠随机分为正常组（12只）和模型制备组（30只），模型制备组大鼠通过饮用5%葡聚糖硫
酸钠溶液构建 UC 模型。将造模成功的大鼠分为模型组、阳性对照组[美沙拉秦肠溶片 270 mg/（kg·d）]、复方芩柏颗粒组[2.52
g/（kg·d）]，每组9只。各组大鼠每天分2次灌胃生理盐水或相应药液，连续3周。灌胃期间，观察各组大鼠一般情况，于末次给药
后进行疾病活动性指数（DAI）评分，并检测其血清中促炎因子（肿瘤坏死因子α、白细胞介素6）和抑炎因子（转化生长因子β1、白细
胞介素 10）水平，观察其结肠组织病理变化并进行病理学评分，检测其粪便中 SCFA（乙酸、丙酸、丁酸）含量以及结肠组织中
GPR41、GPR43、GPR109A蛋白及mRNA的表达情况。结果 与正常组比较，模型组大鼠精神萎靡，粪便带血明显，结肠组织结构
受损严重，可见明显的腺体缺失、水肿、炎症细胞浸润等病理改变；其血清促炎因子水平、DAI评分、结肠病理学评分均显著升高，
而抑炎因子水平，SCFA含量以及GPR41、GPR43、GPR109A蛋白及mRNA的表达均显著降低或下调（P＜0.01）。与模型组比较，
各药物组大鼠一般情况及结肠组织病理改变均有好转，上述定量指标均明显逆转（P＜0.05或P＜0.01）。结论 复方芩柏颗粒能减
轻UC大鼠的肠道炎症及肠道黏膜病理损伤，上述作用可能与其恢复肠道SCFA的含量及其靶点GPR的表达有关。
关键词 复方芩柏颗粒；溃疡性结肠炎；短链脂肪酸；G蛋白偶联受体

Study on the improvement effects of Compound qinbai granules on ulcerative colitis in rats and its
mechanism
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HE Shouyan ，LUO Wenpeng ，PAN Liao ，XIAO Jinyin ，WANG Zhenquan（1. The Third Department of Anal
and Intestinal Medicine， the Second Affiliated Hospital of Hunan University of Chinese Medicine， Changsha
410005， China；2. The Second Department of Anal and Intestinal Medicine，the Second Affiliated Hospital of
Hunan University of Chinese Medicine， Changsha 410005， China；3. Graduate School， Hunan University of
Chinese Medicine， Changsha 410036， China）

ABSTRACT OBJECTIVE To investigate the improvement effects of Compound qinbai granules on ulcerative colitis （UC） in
rats and its mechanism based on short-chain fatty acid （SCFA） and their targets G protein-coupled receptor （GPR）. METHODS
Male SD rats were randomly divided into normal group （12 rats） and model group （30 rats）； the model group was given 5%
dextran sulfate sodium solution to induce the UC model. Model rats were divided into the model group， positive control group
[Mesalazine enteric-coated tablets 270 mg/（kg·d）] and Compound qinbai granules group [2.52 g/（kg·d）]， with 9 rats in each
group. Rats in each group were orally administered with normal saline or corresponding medication twice a day， for three
consecutive weeks. During intragastric administration， the general conditions of rats in each group were observed， and the disease
activity index （DAI） scores were assessed after the last administration. Serum levels of pro-inflammatory cytokines （tumor necrosis
factor- α， interleukin-6） and anti-inflammatory cytokines （transforming growth factor- β1， interleukin-10） were measured. Pathological
changes in their colonic tissues were observed and scored. Additionally， the content of SCFA （acetic acid， propionic acid and
butyric acid） in their feces as well as the protein and mRNA expressions of GPR41， GPR43 and GPR109A in colonic tissues were
detected. RESULTS Compared with the normal group， rats in the model group exhibited lethargy and obvious blood in their feces；
the colonic tissue structure was severely damaged， with pathological changes such as notable glandular loss， edema， and
inflammatory cell infiltration visible； the serum levels of pro-
Δ 基金项目 湖南省自然科学基金项目（No.2023JJ60040）；湖南省
inflammatory cytokines， DAI score and colonic pathology score
中医药科研计划项目（No.2021020）；长沙市自然科学基金项目（No.
were significantly increased， while the levels of anti-inflammatory
kq2202482）
＊第一作者 硕士研究生。研究方向：中医药防治肛肠疾病。E- cytokines， SCFA content， and protein and mRNA expressions
mail：20223238@stu.hnucm.edu.cn of GPR41， GPR43 and GPR109A were significantly decreased
# 通信作者 主任医师，教授，博士生导师，博士。研究方向：中医 or down-regulated （P＜0.01）. Compared with the model group，
药防治肛肠疾病。E-mail：320035@hnucm.edu.cn the general condition and pathological changes of colonic

· 686 · China Pharmacy 2025 Vol. 36 No. 6 中国药房 2025年第36卷第6期