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枳壳中3，5，6，7，8，3′，4′-七甲氧基黄酮对湿阻中焦大鼠的改善
作用研究
Δ
＊
龚文慧 ，谢亚婷，辛 力，严世豪，赵蓓蓓，郑郁清，郭静英，商 杰，郑 鹏，张金莲（江西中医药大学药学院，
#
南昌 330004）
中图分类号 R965；R285 文献标志码 A 文章编号 1001-0408（2024）07-0819-06
DOI 10.6039/j.issn.1001-0408.2024.07.09
摘 要 目的 研究枳壳中3，5，6，7，8，3′，4′-七甲氧基黄酮（HMF）对湿阻中焦大鼠的改善作用。方法 将大鼠随机分为正常组、
模型组、阳性对照组（消旋山莨菪碱片，1 mg/kg）和HMF低、中、高剂量组（0.3、0.6、0.9 mg/kg），每组7只。除正常组外，其余各组大
鼠均采用内因加外因复合因素法造模。造模成功后，各组大鼠灌胃相应药物或生理盐水，每日1次，连续14 d。观察各组大鼠饮
食量、饮水量及精神状态等一般行为学状态，测定大鼠粪便含水率及唾液流率。采用苏木素-伊红染色法观察大鼠胃和小肠组织
的病理形态学变化；检测大鼠血浆中醛固酮含量；检测大鼠胃组织中水通道蛋白3（AQP3）表达水平。结果 与正常组比较，模型
组大鼠饮食量、饮水量均显著减少（P＜0.01），粪便含水率、唾液流率、血浆中醛固酮含量和胃组织中AQP3表达水平均显著增加/
升高（P＜0.01）；大鼠胃组织损伤侵及黏膜肌层，黏膜肌层断裂；小肠出现少量肠绒毛糜烂，腺体结构破坏等病理形态学变化。与
模型组比较，HMF各剂量组大鼠饮食量、饮水量均增加，粪便含水率、唾液流率、血浆中醛固酮含量和胃组织中AQP3表达水平均
减少/降低，上述差异大部分有统计学意义（P＜0.05或P＜0.01）；胃和小肠组织的病理形态学变化均有不同程度改善。结论 枳壳
燥性成分HMF对湿阻中焦大鼠症状有一定的改善作用，其机制可能与降低血浆醛固酮含量、下调胃组织中AQP3表达有关。
关键词 3，5，6，7，8，3′，4′-七甲氧基黄酮；燥性；湿阻中焦；醛固酮；水通道蛋白
Improvement effects of 3，5，6，7，8，3′，4′-heptamethoxyflavone of Fructus Aurantii on rats with damp
blockage of the middle energizer
GONG Wenhui，XIE Yating，XIN Li，YAN Shihao，ZHAO Beibei，ZHENG Yuqing，GUO Jingying，SHANG Jie，
ZHENG Peng，ZHANG Jinlian（School of Pharmacy， Jiangxi University of Chinese Medicine， Nanchang
330004， China）
ABSTRACT OBJECTIVE To investigate the improvement effects of 3，5，6，7，8，3′，4′-heptamethoxyflavone （HMF） of Fructus
Aurantii on rats with damp blockage of the middle energizer. METHODS The rats were randomly divided into normal group，
model group， positive control group （Raceanisodamine tablet， 1 mg/kg）， HMF low-dose， medium-dose and high-dose groups
（0.3， 0.6， 0.9 mg/kg）， with 7 rats in each group. Except for the normal group， the other groups were modeled by internal and
external composite factors. After successful modeling， the rats in each group were given the corresponding drug or normal saline，
once a day， for 14 days. The general behavioral states such as dietary intake， water intake and mental state of the rats were
observed， and the fecal water content rate and saliva flow rate were measured. Hematoxylin-eosin （HE） staining was used to
observe the pathological and morphology in gastric and small intestinal tissues of rats. The plasma content of aldosterone was
detected， and the expression of aquaporins （AQP3） in the gastric tissue of rats was determined. RESULTS Compared with the
normal group， the dietary intake and water intake of the model group rats were significantly decreased （P＜0.01）， the fecal water
content rate， salivary flow rate， plasma content of aldosterone and the expression of AQP3 in gastric tissue were increased
significantly （P＜0.01）. Gastric tissue injury invaded the mucosal muscle layer， resulting in mucosal muscle layer rupture；
pathological and morphological changes such as small
Δ 基金项目 国家自然科学基金项目（No.82060724）；大学生创新 intestinal villous erosion and glandular structure destruction
创业训练计划项目（No.202310412027）；江西省中医药标委会 2021 年 were observed in the small intestine. Compared with the model
第二批标准化项目（No.2021B01） group， the dietary intake and water intake of rats were
＊ 第一作者 硕 士 研 究 生 。 研 究 方 向 ：中 药 炮 制 。 E-mail： increased in HMF groups； fecal water content rate， salivary
2413833758@qq.com
flow rate， plasma content of aldosterone， the expression of
# 通信作者 教授，博士生导师，博士。研究方向：中药学及中药炮
制学。电话：0791-87118995。E-mail：jxjzzjl@163.com AQP3 in gastric tissue were decreased， most of the above
differences were statistically significant （P＜0.05 or P＜0.01）.
中国药房 2024年第35卷第7期 China Pharmacy 2024 Vol. 35 No. 7 · 819 ·
