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艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价
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          齐维金    1, 2* ,刘瑞秀 ,潘淑娟 ,何贤芳 ,王鸿颖 ,王 鲁 (1.贵州大学药学院,贵阳 550025;2.贵州大学
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          西南特色药用生物资源开发利用教育部工程研究中心,贵阳 550025)
          中图分类号  R284.2;R965      文献标志码  A      文章编号  1001-0408(2023)19-2323-05
          DOI  10.6039/j.issn.1001-0408.2023.19.04
          摘  要  目的  优化艾纳香中艾纳香素的提取工艺,并评价其抗菌消炎活性。方法  利用高效液相色谱法测定艾纳香提取液中艾
          纳香素的含量。在单因素实验的基础上,以艾纳香素得率为评价指标,以乙醇体积分数、液料比、提取时间为考察因素,采用Box-
          Behnken响应面法优化艾纳香素的提取工艺并进行验证。使用微量稀释法测定艾纳香素对化脓链球菌、金黄色葡萄球菌、无乳链
          球菌、变异链球菌、枯草芽孢杆菌和藤黄微球菌的抗菌活性。通过小鼠耳廓肿胀实验和毛细血管通透性实验考察艾纳香素的消
          炎活性。结果  最优提取工艺为:乙醇体积分数90%,液料比15∶1,以80 ℃回流提取2 h;采用该提取工艺的艾纳香素得率为1.97
          mg/g。艾纳香素对化脓链球菌、金黄色葡萄球菌、无乳链球菌、变异链球菌、枯草芽孢杆菌、藤黄微球菌的最低抑菌浓度分别为
          50.00、200.00、400.00、400.00、800.00、1 600.00 μg/mL,对化脓链球菌、金黄色葡萄球菌的最低杀菌浓度分别为400.00、1 600.00 μg/mL;
          并能显著抑制二甲苯所致小鼠耳廓肿胀及醋酸所致小鼠毛细血管的通透性(P<0.05或P<0.01)。结论  优化所得的艾纳香素提
          取工艺稳定可行;提取的艾纳香素对化脓链球菌具有一定的抗菌活性,且具有良好的消炎活性。
          关键词  艾纳香;艾纳香素;提取工艺;响应面法;消炎;抗菌

          Optimization  of  extraction  process  and  evaluation  of  the  antibacterial  and  anti-inflammatory  activity  of
          blumeatin from Blumea balsamifera
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          QI Weijin ,LIU Ruixiu ,PAN Shujuan ,HE Xianfang ,WANG Hongying ,WANG Lu(1.  College  of
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          Pharmacy,  Guizhou  University,  Guiyang  550025,  China;2.  Engineering  Research  Center  of  the  Ministry  of
          Education  for  the  Development  and  Utilization  of  Southwest  Characteristic  Medicinal  Biological  Resources,
          Guizhou University, Guiyang 550025, China)
          ABSTRACT   OBJECTIVE  To  optimize  the  extraction  process  of  blumeatin  from  Blumea  balsamifera  and  to  evaluate  its
          antibacterial  and  anti-inflammatory  activity.  METHODS  The  content  of  blumeatin  in  the  extract  of  B.  balsamifera  was  determined
          by HPLC. On the basis of the single factor experiment, the extraction technology of blumeatin was optimized by the Box-Behnken
          response surface method with the volume fraction of ethanol, liquid-solid ratio and extraction time as the factors, using the yield of
          blumeatin  as  index.  Microdilution  method  was  used  to  determine  the  antibacterial  activity  of  blumeatin  against  Streptococcus
          pyogenes,  Staphylococcus  aureus,  Streptococcus  agalactiae,  Streptococcus  mutans,  Bacillus  subtilis  and  Micrococcus  luteus.  The
          anti-inflammatory  activity  of  blumeatin  was  evaluated  by  ear  swelling  test  and  capillary  permeability  test  in  mice.  RESULTS  The
          optimal extraction technology was as follows: ethanol concentration of 90%, liquid-material ratio of 15∶1, extraction time of 2 h at
          80 ℃; the yield of blumeatin using this extraction process was 1.97 mg/g. The minimum inhibitory concentrations of blumeatin for
          S. pyogenes, S. aureus, S. agalactiae, S. mutans, B. subtilis and M. luteus were 50.00, 200.00, 400.00, 400.00, 800.00 and 1 600.00
          μg/mL,  respectively;  the  minimum  bactericidal  concentrations  of  blumeatin  for  S.  pyogenes  and  S.  aureus  were  400.00  and  1  600.00
          μg/mL, respectively. Blumeatin could significantly inhibit the ear swelling induced by xylene and capillary permeability induced by
          acetic acid in mice(P<0.05 or P<0.01). CONCLUSIONS The optimized extraction technology of blumeatin is stable and feasible.
          The extracted blumeatin has a certain antibacterial effect against S. pyogenes and a good anti-inflammatory activity.
          KEYWORDS    Blumea balsamifera; blumeatin; extraction process; response surface method; anti-inflammatory; antibacterial


              艾纳香 Blumea balsamifera(L.)DC. 为菊科艾纳香           的用药历史,是一种重要的民族药。种植户常用水蒸气
          属植物,具有抗肿瘤、抗氧化、消炎、抗菌、镇痛等多种药                         蒸馏法提取艾纳香中的“艾片”和“艾油”,剩下的水溶性
                [1]
          理活性 ,在苗族、黎族、壮族等少数民族地区有着悠久                          物质及药渣常被作为废弃物丢弃,但其废弃物中包含具
                                                             有抗肿瘤、抗血栓等药理活性的黄酮类成分                    [2―3] 。艾纳
             Δ 基金项目 国家自然科学基金项目(No.32160849)                  香素是艾纳香中一种重要的黄酮类化合物,能够对实验
             *第一作者 硕士。研究方向:天然药物成分及生理活性。E-
                                                             性肝损伤起保护作用,对口腔癌细胞及肥大细胞活化脱
          mail:2038298834@qq.com
                                                             颗粒反应均存在有效的抑制作用               [4―5] 。但艾纳香素作为
             # 通信作者 教授,硕士生导师,博士。研究方向:药理学。E-
          mail:wanglu7007@163.com                            艾纳香的主要活性成分之一,是否具有艾纳香那样良好


          中国药房  2023年第34卷第19期                                              China Pharmacy  2023 Vol. 34  No. 19    · 2323 ·
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