Page 45 - 《中国药房》2022年17期

基于熵权TOPSIS法综合评价草果质量 Δ

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郝佳旭 ，李元增 ，范 晓 ，查丽春 ，马云淑 1，2，3，4 # （1.云南中医药大学中药学院，昆明 650500；2.云南省高校
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外用给药系统与制剂技术研究重点实验室，昆明 650500；3.云南省傣医药与彝医药重点实验室，昆明
650500；4.云南省南药可持续利用重点实验室，昆明 650500）

中图分类号 R917 文献标志码 A 文章编号 1001-0408（2022）17-2087-06
DOI 10.6039/j.issn.1001-0408.2022.17.08

摘 要 目的 综合评价不同产地、采收期草果的质量。方法 使用2020年版《中国药典》（四部）中挥发油测定甲法测定草果中总
挥发油含量；采用硝酸铝-亚硝酸钠比色法和福林酚法分别测定草果中总黄酮、总多酚含量；采用气相色谱法测定草果挥发油中α-
蒎烯、β-蒎烯、1，8-桉叶素、α-松油醇、香叶醇、反式橙花叔醇含量；采用超高效液相色谱法测定草果中原儿茶酸、香草酸含量。选择
上述 11 个指标，结合熵权 TOPSIS 法综合评价 16 批草果的质量。结果 16 批草果样品中总挥发油、总黄酮、总多酚、α-蒎烯、β-蒎
烯、1，8-桉叶素、α-松油醇、香叶醇、反式橙花叔醇、原儿茶酸、香草酸的含量分别为 15.833 3～28.000 0 μL/g、29.100 5～78.199 6
mg/g、6.789 8～35.797 7 mg/g、0.088 7～0.401 3 mg/g、0.106 3～0.408 0 mg/g、3.709 6～8.533 1 mg/g、0.259 8～0.599 6 mg/g、
0.314 8～1.324 1 mg/g、0.272 3～0.576 4 mg/g、9.301 2～19.818 5 μg/g、8.180 9～27.666 3 μg/g。熵权 TOPSIS 法分析结果显示，相
对贴近度排名前3位的分别是云南保山7月、云南红河10月和云南文山9月产草果；相对贴近度排名后3位的分别是云南德宏9
月、云南德宏11月、云南德宏12月产草果。结论 云南保山（7月）、红河（10月）、文山（9月）产草果质量较优。
关键词 草果；挥发油；含量测定；熵权TOPSIS法；质量评价
Comprehensive evaluation of the quality of Amomum tsao-ko based on entropy weight TOPSIS method
HAO Jiaxu ，LI Yuanzeng ，FAN Xiao ，ZHA Lichun ，MA Yunshu 1，2，3，4 （1. School of Chinese Materia Medica，
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Yunnan University of Chinese Medicine，Kunming 650500，China；2. Yunnan Colleges and Universities Key
Laboratory of External Drug Delivery System and Preparation Technology，Kunming 650500，China；3. Yunnan
Key Laboratory of Dai and Yi Medicines，Kunming 650500，China；4. Yunnan Key Laboratory of Sustainable
Utilization of Southern Medicine，Kunming 650500，China）

ABSTRACT OBJECTIVE To evaluate the quality of Amomum tsao-ko from different origins and harvesting periods
comprehensively. METHODS The contents of total volatile oil in A. tsao-ko were determined by volatile oil measurement method A
stated in 2020 edition of Chinese Pharmacopoeia（part Ⅳ）；the contents of total flavonoids and total polyphenols in A. tsao-ko
were determined by aluminum nitrate-sodium nitrite colorimetry and folin-ciocalteu method. The contents of α-pinene，β-pinene，
1，8-cineole，α-terpineol，geraniol and trans-nerolidol in the volatile oil of A. tsao-ko were determined by gas chromatography；the
contents of protocatechuate and vanillic acid in A. tsao-ko were determined by ulta high performance liquid chromatography. The
above 11 indicators were selected，and entropy weight TOPSIS method was used to comprehensively evaluate the quality of 16
batches of A. tsao-ko. RESULTS The contents of total volatile oil，total flavonoids，total polyphenols，α-pinene，β-pinene，
1，8-cineole，α-terpineol，geraniol，trans-nerolidol，protocatechuate and vanillic acid in 16 batches of A. tsao-ko were 15.833 3-
28.000 0 μL/g，29.100 5-78.199 6 mg/g，6.789 8-35.797 7 mg/g，0.088 7-0.401 3 mg/g，0.106 3-0.408 0 mg/g，3.709 6-8.533 1
mg/g，0.259 8-0.599 6 mg/g，0.314 8-1.324 1 mg/g，0.272 3-0.576 4 mg/g，9.301 2-19.818 5 μ g/g，8.180 9-27.666 3 μ g/g，
respectively. Entropy weight TOPSIS results showed that the top three of relative closeness rankings were A. tsao-ko produced by
Yunnan Baoshan in July，Yunnan Honghe in October，Yunnan Wenshan in September；the last three of relative closeness rankings
were A. tsao-ko produced by Yunnan Dehong in September，Yunnan Dehong in November，Yunnan Dehong in December.
CONCLUSIONS A. tsao-ko produced by Yunnan Baoshan in July，Yunnan Honghe in October and Yunnan Wenshan in September
present better quality.
KEYWORDS Amomum tsao-ko；volatile oil；content determination；entropy weight TOPSIS method；quality evaluation

草果为姜科植物草果 Amomum tsao-ko Crevost et
Δ 基金项目 国家自然科学基金资助项目（No.82174065） Lemaire的干燥成熟果实，主要分布于我国西南地区，其
＊第一作者 硕士研究生。研究方向：中药药剂学。电话：0351-
中云南为我国草果的主要产区 。草果具有燥湿温中、
[1]
7632549。E-mail：zermattbm@163.com
# 通信作者 教授，硕士生导师，博士。研究方向：中药药剂学。电 截疟除痰的功效，临床常用于辅助治疗癫痫、慢性肾功
[2]
话：0871-65918217。E-mail：yunshuma2@126.com 能衰竭和慢性再生障碍性贫血等疾病 。草果主要含有

中国药房 2022年第33卷第17期 China Pharmacy 2022 Vol. 33 No. 17 ·2087 ·