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内蒙古3种野生藜芦化学成分的定性定量分析 Δ
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韩文杰 ,张 屏,常福厚,梁雨娜,赵红梅,包保全(内蒙古医科大学药学院,呼和浩特 010110)
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中图分类号 R917 文献标志码 A 文章编号 1001-0408(2022)08-0937-07
DOI 10.6039/j.issn.1001-0408.2022.08.06
摘 要 目的 对内蒙古3种野生藜芦(藜芦、毛穗藜芦、兴安藜芦)的化学成分进行定性和定量分析。方法 运用高效液相色谱串
联四极杆-静电场轨道阱高分辨质谱技术,通过查阅SciFinder、ChemSpider数据库和相关文献以及与对照品比对,鉴别藜芦、毛穗
藜芦、兴安藜芦的化学成分。运用高效液相色谱法测定3种野生藜芦中虎杖苷、氧化白藜芦醇和白藜芦醇的含量。结果 共鉴定出
31个化合物,包括13个茋类、11个黄酮类、4个有机酸类、2个糖苷类、1个巴西苏木素类化合物;绝大多数化合物为其中2种或3种
野生藜芦所共有,仅有2个黄酮类化合物山柰酚和木犀草素为兴安藜芦所特有。3种野生藜芦中虎杖苷、氧化白藜芦醇、白藜芦醇
的总含量为6.618~11.292 mg/g,总含量由高到低依次为藜芦、毛穗藜芦、兴安藜芦;虎杖苷、白藜芦醇含量最高的是毛穗藜芦,氧
化白藜芦醇含量最高的是藜芦。结论 3种野生藜芦中大部分成分相似,仅山柰酚和木犀草素为兴安藜芦所特有。虎杖苷、氧化白
藜芦醇和白藜芦醇的含量在3种野生藜芦中存在较大差异。
关键词 藜芦;兴安藜芦;毛穗藜芦;茋类;高效液相色谱串联四极杆-静电场轨道阱高分辨质谱技术;定性分析;定量分析
Qualitative and quantitative analysis of chemical compounds in 3 species of wild Veratrum from Inner
Mongolia
HAN Wenjie,ZHANG Ping,CHANG Fuhou,LIANG Yuna,ZHAO Hongmei,BAO Baoquan(College of
Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)
ABSTRACT OBJECTIVE To conduct qualitative and quantitative analysis for the chemical compounds in 3 species of wild
Veratrum (V. nigrum,V. maackii,V. dahuricum) from Inner Mongolia. METHODS HPLC-Q-Exactive-MS/MS technology was
used to identify the chemical components of V. nigrum,V. maackii and V. dahuricum by consulting SciFinder,ChemSpider database
and related literatures and comparing with the reference substance. The contents of polydatin,oxyresveratrol and resveratrol in 3
species of wild Veratrum were determined by HPLC. RESULTS A total of 31 compounds were identified,including 13 stilbenes,
11 flavonoids,4 organic acids,2 glycosides,1 brasilin. Most of the compounds were shared by 2 or 3 species of wild Veratrum,
only 2 flavonoids kaempferol and luteolin were owned by V. dahuricum. The total contents of polydatin,oxyresveratrol and
resveratrol in 3 species of wild Veratrum were in the range of 6.618-11.292 mg/g,and the total contents of them in V. nigrum were
the highest,followed by V. maackii and V. dahuricum. The contents of polydatin and resveratrol in V. maackii were the highest,and
the content of oxyresveratrol in V. nigrum was highest. CONCLUSIONS Most of the components of 3 species of wild Veratrum are
similar,only kaempferol and luteolin are unique to V. dahuricum. The contents of polydatin,oxyresveratrol and resveratrol are
significantly different among 3 species of wild Veratrum.
KEYWORDS Veratrum nigrum; Veratrum maackii; Veratrum dahuricum; stilbene; HPLC-Q-Exactive-MS/MS; qualitative
analysis;quantitative analysis
蒙药“阿格希日嘎”,中文名藜芦,是藜芦属野生植 毛穗藜芦和兴安藜芦作为藜芦替代品入药的情况 [2-3] 。
物藜芦 Veratrum nigrum Linnaeus 的干燥根及根茎。藜 藜芦属植物的主要成分有茋类、生物碱类、黄酮类等。
芦为蒙医“寒热症总泻药”,用于蒙医八大疗法中的泻下 其中,茋类是具有二苯乙烯基本母核的一类成分,因存
[1]
法,具有重要的地位和药用价值 。藜芦的同属不同种 在较多的酚羟基而具有很强的抗氧化活性,为藜芦的主
植物毛穗藜芦 V. maackii Regel 和兴安藜芦 V. dahuricum 要活性成分之一,典型代表成分是白藜芦醇及其类似
(Turcz.)Loes. f.与其交叉分布,3种野生藜芦形态相近, 物 ;此外,该类成分还具有抗炎、抗肿瘤、激活巨噬细
[4]
易出现混淆使用的现象,同时内蒙古部分地区也存在将 胞、抗血小板聚集、抗过敏、消除自由基等作用 [5-8] 。本
Δ 基金项目:国家自然科学基金地区科学基金项目(No.81760- 文以藜芦属植物特征性茋类成分为主要研究对象,利用
9111) 高效液相色谱串联四极杆-静电场轨道阱高分辨质谱
*硕士研究生。研究方向:中蒙药药理学。E-mail:2670440491@
(high-performance liquid chromatography tandem quadru-
qq.com
pole-electrostatic field orbitrap high-resolution mass spec-
# 通信作者:教授,硕士生导师,博士。研究方向:天然药化、蒙药
物质基础和资源可持续利用。E-mail:baobaoquan123@yeah.net trometry,HPLC-Q-Exactive-MS/MS)技术,对内蒙古3种
中国药房 2022年第33卷第8期 China Pharmacy 2022 Vol. 33 No. 8 ·937 ·