Page 68 - 《中国药房》2022年7期

不同炮制方法对衢枳壳药效指标成分含量及抗氧化活性的影响 Δ

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王 璐 ，汪丽霞 ，吴婷婷 ，赵四清 ，郑美瑜 ，陆胜民 （1.浙江省农业科学院食品科学研究所，杭州
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310021；2.浙江省常山县农业特色产业发展中心，浙江常山 324200；3.中国计量大学生命科学学院，杭州 310018）
中图分类号 R943.1；R283.1 文献标志码 A 文章编号 1001-0408（2022）07-0830-06
DOI 10.6039/j.issn.1001-0408.2022.07.11
摘 要 目的 研究不同炮制方法对衢枳壳中药效指标成分含量及其抗氧化活性的影响。方法 根据2020年版《中国药典》（四部）
炮制通则和2015年版《浙江省中药炮制规范》中相关炮制方法制备衢枳壳的蒸制、煮制、醋炙、酒炙、麸炒、麸制、蜜制样品。检测
不同衢枳壳样品中水分、灰分的含量；采用高效液相色谱法测定不同衢枳壳样品中药效指标成分柚皮苷和新橙皮苷的含量；采用
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DPPH、ABTS 自由基清除实验和总还原力实验考察不同衢枳壳样品的抗氧化活性。结果 衢枳壳不同样品中的水分、灰分、柚皮
苷、新橙皮苷含量均符合2015年版《浙江省中药炮制规范》中的相关规定，其中柚皮苷的含量从高到低依次为未炮制样品＞蜜制样
品＞麸制样品＞煮制样品＞醋炙样品＞酒炙样品＞麸炒样品＞蒸制样品，新橙皮苷含量从高到低依次为未炮制样品＞煮制样品＞
麸制样品＞蜜制样品＞醋炙样品＞蒸制样品＞酒炙样品＞麸炒样品。衢枳壳煮制、麸制和麸炒样品对DPPH自由基的清除能力
较好[半数抑制浓度（IC50 ）分别为 7.49、8.37、10.22 mg/mL]，煮制、蒸制、麸制样品对 ABTS 自由基的清除能力较好（IC50分别为
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1.76、2.03、2.72 mg/mL）；另外，与酒炙和蜜制样品相比，衢枳壳未炮制样品及其他炮制品均具有较好的总还原能力。结论 衢枳壳
经不同方法炮制后的药效指标成分含量均有所降低，但均符合2015年版《浙江省中药炮制规范》中的相关规定，且部分炮制品的
抗氧化活性较未炮制样品有所增强。
关键词 衢枳壳；炮制；含量测定；抗氧化活性

Effects of different processing methods on the contents of the pharmacodynamic index components and
antioxidant activity of Citrus aurantium
WANG Lu ，WANG Lixia ，WU Tingting ，ZHAO Siqing ，ZHENG Meiyu ，LU Shengmin（1. Institute of Food
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Science， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China； 2. Changshan County
Agricultural Characteristic Industry Development Center of Zhejiang Province，Zhejiang Changshan 324200，
China；3. College of Life Sciences，China Jiliang University，Hangzhou 310018，China）
ABSTRACT OBJECTIVE To study the effects of different processing methods on the contents of the pharmacodynamic index
components in Citrus aurantium and their antioxidant activity. METHODS According to the general principles of 2020 edition of
Chinese Pharmacopoeia （volume Ⅳ） and the relevant processing methods in 2015 edition of Processing Specifications of
Traditional Chinese Medicine in Zhejiang Province，the samples of C. aurantium were prepared by steaming with water，boiling
with water，stir-frying with vinegar，stir-frying with wine，stir-frying with bran，processing with bran and processing with honey.
The contents of moisture and ash in different products of C. aurantium were detected. The contents of naringin and neohesperidin in
different products of C. aurantium were determined by high performance liquid chromatography. The antioxidant activity of different
products was investigated by DPPH and ABTS radical scavenging experiments and the total reducing power test. RESULTS The
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contents of moisture，ash，naringin and neohesperidin were in line with the relevant requirements in 2015 edition of Processing
Specifications of Traditional Chinese Medicine in Zhejiang Province. The content of naringin in descending order was as follow：
unprocessed sample＞sample processed with honey＞sample processed with bran＞sample boiled with water＞sample stir-fried with
vinegar＞sample stir-fried with wine＞sample stir-fried with bran＞sample steamed with water. The content of neohesperidin in
descending order was as follow：unprocessed sample＞sample boiled with water＞sample processed with bran＞sample processed
with honey＞sample stir-fried with vinegar＞sample steamed with water＞sample stir-fried with wine＞sample stir-fried with bran.
The samples after boiling with water，processing with bran，and stir-fried with bran had better DPPH radicals scavenging ability
（IC50 were 7.49，8.37 and 10.22 mg/mL，respectively）. The samples after boiling with water，steaming with water，and processed
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with bran had better ABTS radicals scavenging ability （IC50
Δ 基 金 项 目 ：浙 江 省 农 业 重 大 技 术 协 同 推 广 计 划 项 目
were 1.76，2.03 and 2.72 mg/mL，respectively）. In addition，
（No.2020XTTGZYC03）
compared with sample stir-fried with wine and processed with
＊助理研究员，博士。研究方向：药用植物活性成分研究与开
发。E-mail：wanglu1286@163.com honey，unprocessed sample and other processed products of C.
# 通信作者：研究员，博士。研究方向：植物活性成分加工与综合 aurantium had better total reducing ability. CONCLUSIONS
利用。E-mail：lushengmin@hotmail.com After processing，the contents of the main pharmacodynamic

·830 · China Pharmacy 2022 Vol. 33 No. 7 中国药房 2022年第33卷第7期