Page 63 - 《中国药房》2022年5期

虎杖一体化饮片与传统饮片的指纹图谱及体外抗氧化活性比较 Δ

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简祈盼 ，朱映睿 ，郑宇锟 ，成焕波 ，涂济源 ，王光忠 （1.湖北中医药大学药学院，武汉 430065；2.湖北省中
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西医结合医院药学部，武汉 430021）
中图分类号 R283.1 文献标志码 A 文章编号 1001-0408（2022）05-0569-06
DOI 10.6039/j.issn.1001-0408.2022.05.10
摘 要 目的 比较虎杖产地加工与炮制一体化（简称“一体化”）制备的饮片与传统饮片的指纹图谱及体外抗氧化活性差异。方
法 根据一体化工艺和传统工艺分别制备10批虎杖一体化饮片和10批传统饮片。采用高效液相色谱法建立虎杖两种饮片的指纹
图谱，并进行比较；检测虎杖两种饮片对1，1-二苯基-2-三硝基苯肼（DPPH）自由基、ABTS自由基、超氧自由基、羟自由基的清除率
以及对Fe 的还原能力，比较这两种饮片的体外抗氧化活性。结果 虎杖两种饮片共有11个共有峰，其中一体化饮片共有17个共
3+
有峰，传统饮片共有13个共有峰；虎杖一体化饮片中6号峰（虎杖苷）和15号峰（大黄素-8-O-β-D-葡萄糖苷）的峰高明显高于传统
饮片，传统饮片中13号峰（白藜芦醇）、17号峰（大黄素）和19号峰（大黄素甲醚）的峰高明显高于一体化饮片。体外抗氧化活性结
3+
果显示，虎杖一体化饮片和传统饮片对DPPH自由基、ABTS自由基、超氧自由基和羟基自由基均具有清除能力，对Fe 也均具有
还原能力。结论 虎杖一体化炮制工艺较传统炮制工艺可更好地保留虎杖的有效成分，且虎杖一体化饮片的体外抗氧化活性强于
传统饮片。
关键词 虎杖；产地加工与炮制一体化；指纹图谱；抗氧化活性

Comparison of the fingerprints and in vitro antioxidant activity of decoction pieces of Polygonum
cuspidatum by integrated processing and traditional processing
JIAN Qipan ，ZHU Yingrui ，ZHENG Yukun ，CHENG Huanbo ，TU Jiyuan ，WANG Guangzhong（1.School of
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Pharmacy，Hubei University of Chinese Medicine，Wuhan 430065，China；2. Dept. of Pharmacy，Hubei Hospital
of Integrated Traditional Chinese and Western Medicine，Wuhan 430021，China）
ABSTRACT OBJECTIVE To compare the differences of the fingerprint and in vitro antioxidant activity between decoction
pieces of Polygonum cuspidatum by integrated technology of habitat processing and processing（short for IPDP）and traditional
processing decoction pieces （short for TPDP）. METHODS Ten batches of IPDP and ten batches of TPDP were prepared by
integrated technology and traditional technology，respectively. HPLC method was used to establish and compare the fingerprints of
IPDP and TPDP. The scavenging rates of DPPH free radical，ABTS free radical，superoxide free radical and hydroxyl free radical
and reducing activity of Fe 3 + were detected for IPDP and TPDP. In vitro antioxidant activities were compared between IPDP and
TPDP. RESULTS There were 11 common peaks in the fingerprints of IPDP and TPDP，among which 17 came from IPDP and 13
came from TPDP. The peak heights of peak 6（polydatin）and peak 15（emodin-8-O-β-D-glucoside）in IPDP were significantly
higher than those in the TPDP，and the peak heights of peak 13（resveratrol），peak 17（emodin）and peak 19（physcion）in the
TPDP were significantly higher than those in the IPDP. The results of in vitro antioxidant test showed that IPDP and TPDP had a
certain scavenging capacity on DPPH free radical，ABTS free radical，superoxide free radical and hydroxyl free radicals，and also
had a certain reducing capacity on Fe . CONCLUSIONS The integrated processing technology of P. cuspidatum has a good
3 +
retention effect on the glycosides in P. cuspidatum，and the in vitro antioxidant activity of IPDP is stronger than that of TPDP.
KEYWORDS Polygonum cuspidatum；integrated technology of habitat processing and processing；fingerprint；antioxidant activity

虎杖为蓼科植物虎杖Polygonum cuspidatum Sieb.et 调血脂、抗炎、抗氧化、抗菌、抗病毒以及抗肿瘤等药理
Zucc.的干燥根和根茎，归肝、胆、肺经，具有清热解毒、散 活性 [3－8] 。
瘀止痛、止咳化痰、利湿退黄的功效 ，多用于治疗血症、 虎杖在制成饮片时，需要经过药材采挖→净制→干
[1]
湿热黄疸、淋症、闭经、跌打损伤、肺热咳嗽、毒蛇咬伤及 燥（制成药材）→软化→切制→再干燥（制成饮片）的加
外伤出血等 。现代药理学研究表明，虎杖具有抗血栓、 工过程，这不仅耗时较长，而且在干燥和软化的过程中
[2]
可能会造成有效成分损失和药材霉变。为了保证中药
Δ 基金项目：国家重点研发计划项目（No.2017YFC1701003）
饮片质量，解决药用植物到中药饮片生产环节中的加工
＊硕士研究生。研究方向：中药炮制工艺、质量控制及原理。
复杂、掺杂、掺假等问题，相关专家学者提出了药材产地
E-mail：1915717793@qq.com
# 通信作者：教授，硕士生导师，博士。研究方向：中药炮制。 加工与炮制一体化（简称“一体化”）的想法 [9－10] 。本课题
E-mail：wgzhong4067@sina.com 组前期调研发现，将虎杖进行一体化工艺处理能显著减

中国药房 2022年第33卷第5期 China Pharmacy 2022 Vol. 33 No. 5 ·569 ·