Page 24 - 《中国药房》2022年2期

·药学研究·

知母栽培品和野生品的指纹图谱建立及差异性成分确认 Δ

常鑫鑫 ，李 倩 ，薛紫鲸 ，景松松 ，赵艳云 ，郑玉光 ，宋军娜 （1.河北中医学院药学院/河北省中药炮制技术
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创新中心，石家庄 050200；2.河北化工医药职业技术学院，石家庄 050200）
中图分类号 R282 文献标志码 A 文章编号 1001-0408（2022）02-0146-07
DOI 10.6039/j.issn.1001-0408.2022.02.04
摘 要 目的 建立知母栽培品和野生品的指纹图谱，确认两者的差异性成分。方法 采用蒸发光散射检测器，以高效液相色谱法
结合《中药色谱指纹图谱相似度评价系统（2012版）》建立14批知母栽培品和14批知母野生品的指纹图谱，并进行相似度评价，通
过与混合对照品色谱图比对指认共有峰。同法测定知母栽培品和野生品中指认共有峰对应成分的含量，通过主成分分析和正交
偏最小二乘法-判别分析确认两者的差异性成分，并比较其含量。结果 在28批知母药材中共有10个共有峰，指认出新芒果苷（峰
1）、芒果苷（峰2）、异芒果苷（峰3）、知母皂苷BⅡ（峰7）、知母皂苷BⅢ（峰8）、知母皂苷Ⅰ（峰9）、知母皂苷AⅢ（峰10）7个成分；各
样品指纹图谱与对照指纹图谱的相似度均不低于0.963。知母栽培品与野生品中7个成分的平均总含量分别为74.18、84.72 mg/g，
二者比较差异有统计学意义（P＜0.05）。知母栽培品与野生品可被分为两类，差异性成分为新芒果苷、芒果苷、知母皂苷BⅡ、知母皂
苷AⅢ（变量投影重要性值均大于1）。知母栽培品中新芒果苷含量明显高于野生品（P＜0.05），芒果苷、知母皂苷BⅡ和知母皂苷AⅢ
含量均明显低于野生品（P＜0.05）。结论 成功建立了知母的指纹图谱，并初步确认了知母栽培品和野生品的差异性成分。
关键词 知母；野生品；栽培品；指纹图谱；多成分定量分析；差异性成分

Establishment of fingerprints and differential component identification of cultivated and wild Anemarrhena
asphodeloides
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CHANG Xinxin ，LI Qian ，XUE Zijing ，JING Songsong ，ZHAO Yanyun ，ZHENG Yuguang ，SONG Junna（1.
College of Pharmacy，Hebei University of Chinese Medicine/Hebei Traditional Chinese Medicine Processing
Technology Innovation Center，Shijiazhuang 050200，China；2. Hebei Chemical and Pharmaceutical College，
Shijiazhuang 050200，China）
ABSTRACT OBJECTIVE To establish the fingerprints of cultivated and wild Anemarrhena asphodeloides，and to identify their
differential components. METHODS Using an evaporative light-scattering detector， the high performance liquid chromatography
combined with Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition） were used to establish
fingerprints of 14 batches of cultivated A. asphodeloides and 14 batches of wild medicinal materials，and evaluate their similarity.
The common peaks were identified by comparison with the chromatogram of the mixed control. At the same time，the contents of
components corresponding to common peaks in cultivated and wild A. asphodeloides were determined. The principal component
analysis and orthogonal partial least squares discrimination analysis were adopted to identify differential components of them，and
compare the contents of them. RESULTS Among 28 batches of A. asphodeloides， 10 common peaks were found， i.e.
neomangiferin（peak 1），mangiferin（peak 2），isomangiferin（peak 3），timosaponin BⅡ（peak 7），timosaponin BⅢ（peak 8），
timosaponin Ⅰ（peak 9），timosaponin AⅢ（peak 10）. The similarities of fingerprints of samples with control fingerprint were no
less than 0.963. The average total contents of seven components in cultivated and wild A. asphodeloides were 74.18 and 84.72 mg/g，
respectively；there was statistical significance （P＜0.05）. The cultivated and wild A. asphodeloides could be divided into two
categories. The differential components were neomangiferin，mangiferin，timosaponin BⅡ and timosaponin AⅢ（VIP values were
all higher than 1）. The content of neomangiferin in cultivated products was significantly higher than that in wild products（P＜
0.05），and the contents of mangiferin，timosaponin BⅡ and timosaponin AⅢ were significantly lower than those in wild products
（P＜0.05）. CONCLUSIONS Fingerprint of A. asphodeloides
Δ 基金项目：国家自然科学基金资助项目（No.81803762）；河北省
is established，and differential components of cultivated and
高校百名优秀创新人才支持计划资助项目（No.SLRC2019044）；河北
wild A. asphodeloides are identified primarily.
省高层次人才资助项目（No.A201901037）
＊硕士研究生。研究方向：中药资源与炮制。E-mail：949049932@ KEYWORDS Anemarrhenae asphodeloides；wild product；
qq.com cultivated product；fingerprints；multi-component quantitative
# 通信作者：副教授，硕士生导师，硕士。研究方向：中药活性成 analysis；differential components
分分析及其对心血管疾病的作用机制。E-mail：junnasong@163.com

·146 · China Pharmacy 2022 Vol. 33 No. 2 中国药房 2022年第33卷第2期