Page 81 - 《中国药房》2021年21期

延龄草总皂苷对佐剂性关节炎模型大鼠的改善作用及机制 Δ

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谭晓茜 ，史伟文 ，蒋安蓉 ，柳 蔚 ，李世刚 ，喻玲玲 （1.三峡大学医学院，湖北 宜昌 443002；2.宜昌市中医
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医院药剂科，湖北 宜昌 443002）
中图分类号 R285.5 文献标志码 A 文章编号 1001-0408（2021）21-2635-05
DOI 10.6039/j.issn.1001-0408.2021.21.13
摘 要 目的：研究延龄草总皂苷（TTM）对佐剂性关节炎模型大鼠的改善作用及机制。方法：将 SD 大鼠随机分为造模组（n＝
44）和空白组（n＝6），造模组大鼠注射完全弗氏佐剂以复制佐剂性关节炎模型，空白组大鼠同法注射等体积生理盐水。将造模成
功的大鼠分为模型组、雷公藤多苷片组（40 mg/kg）和TTM低、中、高剂量组（50、100、200 mg/kg），每组6只。各给药组大鼠灌胃相
应药物，空白组和模型组大鼠灌胃生理盐水，每天1次，连续9 d。末次灌胃后，测定大鼠体质量、足肿胀度（双侧），并进行关节炎
性评分；测定大鼠血清中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6含量；观察大鼠踝关节病理形态学变化；检测大鼠膝
关节组织中NOD样受体蛋白3（NLRP3）、凋亡相关斑点样蛋白（ASC）、胱天蛋白酶1（caspase-1）的蛋白表达水平；检测大鼠踝关节
滑膜组织中NLRP3的蛋白表达水平。结果：与空白组比较，模型组大鼠双侧足肿胀度，血清中TNF-α、IL-1β、IL-6含量，膝关节组
织中NLRP3、ASC、caspase-1蛋白表达水平，踝关节滑膜组织中NLRP3蛋白表达水平均显著升高（P＜0.05或P＜0.01）；体质量显
著降低（P＜0.05）；滑膜细胞增生明显，可见滑膜组织充血、炎性细胞浸润等。与模型组比较，TTM各剂量组大鼠上述指标水平大
部分均显著逆转（P＜0.05或P＜0.01）；滑膜细胞增生、滑膜组织充血及软骨细胞破坏等病理改变均减轻，炎症细胞浸润减少。结
论：TTM 可改善大鼠类风湿性关节炎；其作用机制可能与抑制 NLRP3/caspase-1 信号通路的活性，降低炎症因子 TNF-α、IL-1β、
IL-6的表达有关。
关键词 延龄草总皂苷；关节炎；NOD样受体蛋白3；抗炎；大鼠

Improvement Effects of Trillium tschonoskii Total Saponins on Adjuvant-induced Arthritis Model Rats and
Its Mechanism
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TAN Xiaoxi ，SHI Weiwen ，JIANG Anrong ，LIU Wei ，LI Shigang ，YU Lingling（1. Medical College，China
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Three Gorges University，Hubei Yichang 443002，China；2. Dept. of Pharmacy，Yichang Hospital of TCM，
Hubei Yichang 443002，China）
ABSTRACT OBJECTIVE：To study the improvement effects of Trillium tschonoskii total saponins（TTM）on adjuvant-induced
arthritis model rats and its mechanism. METHODS：SD rats were randomly divided into model group（n＝44）and blank group
（n＝6）. Model group was given Complete Freund’s adjuvant to induce the adjuvant-induced arthritis model；blank group was given
constant volume of normal saline with same method. Model group were divided into model group，Tripterygium wilfordii
polyglycoside tablets group（40 mg/kg），TTM low-dose，medium-dose and high-dose groups（50，100，200 mg/kg），with 6 rats
in each group. Administration groups were given relevant medicine intragastrically；blank group and model group were given
normal saline intragastrically，once a day，for consecutive 9 days. After last intragastric administration，the body weight and foot
swelling degree（bilateral）were detected，and arthritis score was performed. The contents of tumor necrosis fator α（TNF-α），
interleukin 1β（IL-1β）and IL-6 were detected；the pathomorphological changes of rat ankle were observed；protein expression of
NOD-like receptor protein 3 （NLRP3），apoptosis associated spot-like protein （ASC），caspase-1 were detected；the protein
expression of NLRP3 in synovial tissue of ankle joint were also determined. RESULTS：Compared with blank group，foot swelling
degree，serum contents of TNF-α，IL-1β and IL-6，the protein expression of NLRP3，ASC and caspase-1 in knee tissue as well as
the protein expression of NLRP3 in synovial tissue of ankle joint were increased significantly in model group（P＜0.05 or P＜
0.01），while the body weight was decreased significantly（P＜0.05）. The obvious proliferation of synovial cells，the congestion
and inflammatory cell infiltration of synovial tissue were all observed. Compared with model group，most of the above indexes
were all reversed significantly in TTM groups（P＜0.05 or P＜0.01）；the pathological changes such as synovial cell proliferation，
congestion of synovial tissue and chondrocyte destruction were
Δ 基金项目：宜昌市医疗卫生研究项目（No.A21-2-051）
all relieved，and inflammatory cell infiltration was alleviated.
＊药师，硕士研究生。研究方向：中药有效性评价。E-mail：
CONCLUSIONS：TTM can improve rheumatoid arthritis of
784249346@qq.com
# 通信作者：副教授，硕士生导师，博士。研究方向：天然药物应 rats， the mechanism of which may be associated with
用。E-mail：yulingling@ctgu.edu.cn inhibiting the activity of NLRP3/caspase-1 signaling pathway

中国药房 2021年第32卷第21期 China Pharmacy 2021 Vol. 32 No. 21 ·2635 ·