Page 70 - 《中国药房》2021年21期

瑶药消瘤藤的质量标准研究 Δ

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黄建猷 1，2＊ ，胡筱希 ，谭 晓 ，屈信成 ，陆国寿 1，2 # ，黄周锋（1.广西壮族自治区中医药研究院，南宁 530022；
2.广西中药质量标准研究重点实验室，南宁 530022）
中图分类号 R284.1 文献标志码 A 文章编号 1001-0408（2021）21-2624-07
DOI 10.6039/j.issn.1001-0408.2021.21.11

摘 要 目的：建立瑶药消瘤藤的质量标准，为其质量控制提供参考。方法：采集10批广西产消瘤藤药材，观察其性状，并对其
根、茎的横切面以及药材粉末进行显微鉴别；以7-羟基香豆素为指标成分建立该药材的薄层色谱（TLC）鉴别方法；测定该药材中
水分、总灰分、酸不溶性灰分和醇溶性浸出物；采用高效液相色谱（HPLC）法同时测定该药材中茵芋苷、7-羟基香豆素和7-羟基-8-
甲氧基香豆素的含量。结果：消瘤藤根和茎表面皱纹明显、质硬，气微、味微苦，横切面有分泌细胞和针晶细胞分布；药材粉末中有
草酸钙针晶、草酸钙方晶、石细胞和淀粉粒。TLC结果显示，供试品色谱中，在与7-羟基香豆素对照品色谱相应的位置上，显相同
颜色的荧光斑点。10批药材的水分测定结果为9.1％～11.8％，总灰分为3.6％～6.7％，酸不溶性灰分为0.10％～0.44％，醇溶性浸
出物为8.9％～14.0％。茵芋苷、7-羟基香豆素和7-羟基-8-甲氧基香豆素的质量浓度分别在6.088～182.640、1.568～47.040、1.096～
32.880 μg/mL范围内与其对应色谱峰峰面积成良好的线性关系（r均不低于0.999 7）；平均加样回收率分别为 99.47％、101.07％、
99.89％（RSD均小于2％）；精密度、稳定性（24 h）、重复性、耐用性试验的RSD均小于2％。茵芋苷等3种待测成分的含量分别为
1.337～9.534、0.348～2.236、0.083～1.088 mg/g。结论：初步拟定消瘤藤中水分不得过12.0％、总灰分不得过7.0％、醇溶性浸出物
不得少于8.0％、茵芋苷含量不得少于1.30 mg/g、7-羟基香豆素含量不得少于0.30 mg/g、7-羟基-8-甲氧基香豆素含量不得少于0.06
mg/g。
关键词 瑶药；消瘤藤；质量标准；薄层色谱法；高效液相色谱法；显微鉴别；性状鉴别；茵芋苷；7-羟基香豆素；7-羟基-8-甲氧基香豆素

Study on Quality Standard of Yao Medicine Pileostegia tomentella
HUANG Jianyou ，HU Xiaoxi ，TAN Xiao ，QU Xincheng ，LU Guoshou ，HUANG Zhoufeng（1.Guangxi
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Zhuang Autonomous Region Institute of Chinese Medicine & Pharmaceutical Sciences，Nanning 530022，China；
2. Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards，Nanning 530022，China）
ABSTRACT OBJECTIVE：To establish the quality standard of Yao medicine Pileostegia tomentella，and to provide reference for
its quality control. METHODS：Totally 10 batches of P. tomentella were collected from Guangxi area. The properties of P.
tomentella were observed and microscopic identification was conducted for the transverse section of its stems，roots and powder.
TLC method was established by using 7-hydroxycoumarin as reference. The contents of water，total ash，acid-insoluble ash and
alcohol-soluble extract in P. tomentella were determined. The contents of skimmin，7-hydroxycoumarin and 7-hydroxy-8-
methoxycoumarin in P. tomentella were determined by HPLC. RESULTS：The root and stem surface of P. tomentella possessed
obvious wrinkles，hard texture，slight smell and bitter taste，and there were secretory cells and needle crystal cells in the cross
section. The medicinal powder contained calcium oxalate needle crystal，calcium oxalate square crystal，sclerotic cell and starch
granules. The results of TLC showed that the spots of the same color were found in the corresponding positions of chromatograms
of test samples and 7-hydroxycoumarin control. In the 10 batch of P. tomentella，the contents of water were 9.1％-11.8％；those of
total ash were 3.6％-6.7％；those of acid-insoluble ash were 0.10％-0.44％；those of alcohol-soluble extract were 8.9％-14.0％.The
linear range of skimmin，7-hydroxycoumarin and 7-hydroxy-8-methoxycoumarin were 6.088-182.640，1.568-47.040 and 1.096-
32.880 μg/mL（all r not less than 0.999 7）. The average recoveries were 99.47％，101.07％ and 99.89％（RSD were all less than
2％）. RSDs of precision，stability（24 h），reproducibility and
Δ 基 金 项 目 ：广 西 自 然 科 学 基 金 资 助 项 目（No.2020GXNS- durability tests were all lower than 2％ . The contents of 3
FAA259067）；广西中医药适宜技术开发与推广项目（No.GZSY20- components such as skimmin were 1.337-9.534，0.348-2.236，
04）；广西中药质量标准研究重点实验室自主研究课题（No.桂中重自
0.083-1.088 mg/g，respectively. CONCLUSIONS：It is preli-
201705）
minarily proposed that the content of water in P. tomentella is
＊副主任药师。研究方向：中药药效物质基础及质量标准。电
not more than 12.0％ ；that of total ash is not more than
话：0771-5868986。E-mail：dearhuangjianyou@126.com
# 通信作者：副主任药师。研究方向：中药学。电话：0771-5868986。 7.0％；that of alcohol-soluble extract is not less than 8.0％；
E-mail：luguoshou@foxmail.com that of skimmin is not less than 1.30 mg/g；that of 7-hydroxy-

·2624 · China Pharmacy 2021 Vol. 32 No. 21 中国药房 2021年第32卷第21期