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基于高效液相色谱和红外光谱的生三七及其炮制品的鉴别 Δ
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李雨昕 ,邢 娜 ,张志宏 ,于天颖 ,马恩耀 ,王 雪 ,白浩东 ,曾元宁 ,王秋红 (1.黑龙江中医药大学药学
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院/教育部北药基础与应用研究重点实验室/黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨
150040;2.广东药科大学中药学院,广州 510006;3.广州采芝林药业有限公司,广州 510145)
中图分类号 R284 文献标志码 A 文章编号 1001-0408(2021)18-2194-09
DOI 10.6039/j.issn.1001-0408.2021.18.04
摘 要 目的:鉴别生三七及其炮制品。方法:采用高效液相色谱法(HPLC)建立指纹图谱。以人参皂苷Rb1为参照,采用《中药
色谱指纹图谱相似度评价系统(2012版)》绘制15批生三七及其炮制品的HPLC指纹图谱并进行相似度评价,通过与对照品对比
确定共有峰。采用SPSS 21.0、SIMCA 14.1软件进行聚类分析、主成分分析、正交偏最小二乘法分析,以变量重要性投影(VIP)值
大于1为标准,筛选造成生三七及其炮制品质量差异的标志性成分。采用OMNIC 8.2.0软件建立生三七及其炮制品的红外(IR)指
纹图谱并进行相似度评价,采用双指标序列分析法对15批生三七及其炮制品的IR指纹图谱吸收峰进行分析。结果:15批生三七
有16个共有峰,相似度为0.911~1.000;炮制品有25个共有峰,相似度为0.862~1.000;二者共有12个相同的共有峰,并指认其中
3 个共有峰,分别为三七皂苷 R1、人参皂苷 Rg1、人参皂苷 Rb1。聚类分析结果显示,当距离为 10 时,15 批生三七可聚为两类,
SW1~SW5 聚为一类,SH1~SH5、SQ1~SQ5 聚为一类;15 批炮制品 ZW1~ZW5、ZH1~ZH5、ZQ1~ZQ5 聚为一类。当距离为 5
时,15批生三七可聚为3类,SW1~SW5聚为一类,SH2~SH5、SQ2聚为一类,SQ1、SQ3~SQ5、SH1聚为一类;15批炮制品可聚为
两类,ZW1~ZW5 聚为一类,ZH1~ZH5、ZQ1~ZQ5 聚为一类。主成分分析结果显示,前两个主成分的累计方差贡献率为
80.104%。正交偏最小二乘法分析结果显示,5个峰的VIP值大于1,分别为峰H、峰G、峰J、峰F(人参皂苷Rg1 )、峰I。15批生三七
及其炮制品 IR 指纹图谱的相似度分别为 0.889 7~1.000 0、0.972 8~1.000 0;共有峰率均为 80%~100%,变异峰率分别为 0~
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17.65%、0~18.75%。经吸收峰波数对比发现,生三七在3 440、1 450 cm 处,炮制品在1 530、575 cm 处存在差异。结论:所建
HPLC指纹图谱和IR指纹图谱的相似度均较好,能有效区分生三七及其炮制品。不同产地生三七及其炮制品的共有峰率高、变异
率低,二者化学成分不同;峰H、峰G、峰J、人参皂苷Rg1、峰I为引起生三七及其炮制品质量差异的标志性成分。
关键词 三七;炮制品;高效液相色谱法;红外光谱;不同产地
Identification of Panax notoginseng and Its Processed Products Based on HPLC and IR Spectrum
LI Yuxin ,XING Na ,ZHANG Zhihong ,YU Tianying ,MA Enyao ,WANG Xue ,BAI Haodong ,ZENG
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Yuanning ,WANG Qiuhong (1. School of Pharmacy,Heilongjiang University of Traditional Chinese Medicine/
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Key Laboratory of Foundation and Application Research of Northern Traditional Chinese Medicine,Ministry of
Education/Heilongjiang Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic
Material Bases,Harbin 150040,China;2. School of Traditional Chinese Medicine,Guangdong Pharmaceutical
University,Guangzhou 510006,China;3. Guangzhou Caizhilin Pharmaceutical Co. Ltd.,Guangzhou 510145,
China)
ABSTRACT OBJECTIVE: To identify Panax notoginseng and its processed products. METHODS: The fingerprint was
established by HPLC. Using ginsenoside Rb1 as reference,HPLC fingerprints of 15 batches of P. notoginseng and its processed
products were drawn and the similarity evaluation was conducted by using the Similarity Evaluation System for TCM Chromatographic
Fingerprints(2012 edition). The common peaks were confirmed by comparing with substance control. SPSS 21.0 and SIMCA 14.1
software were used to perform cluster analysis,principal component analysis and orthogonal partial least squares-discriminant
analysis;taking the variable importance projection(VIP)value greater than 1 as the standard,the differential marker components
causing the quality difference between P. notoginseng and its processed products were screened. IR fingerprints of P. notoginseng
and its processed products were established by OMNIC 8.2.0 software,and the spectral similarity was evaluated;double index
sequence analysis was used to analyze absorption peaks of IR fingerprints of 15 batches of P. notoginseng and its processed
products. RESULTS:There were 16 common peaks in the
Δ 基金项目:国家重点研发计划项目(No.2018YFC1707100)
fingerprints of 15 batches of P. notoginseng, and the
*硕士研究生。研究方向:中药炮制原理及饮片质量。电话:
similarities were 0.911-1.000;there were 25 common peaks in
020-39353241。E-mail:1004099453@qq.com
# 通信作者:教授,博士生导师,博士。研究方向:中药炮制、中药 the fingerprints of processed products, and the similarities
药 效 物 质 基 础 和 作 用 机 制 。 电 话 :020-39353241。 E-mail:qh- were 0.862-1.000. They had 12 identical common peaks,and
wang668@sina.com three of them were identified as sanchinoside R1,ginsenoside
·2194 · China Pharmacy 2021 Vol. 32 No. 18 中国药房 2021年第32卷第18期