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·药学研究·

艳山姜提取物对急性胃溃疡模型小鼠的保护作用研究 Δ

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魏 晴 ，梁珊珊 ，熊 瑞 ，魏 娜 ，薛 娟 （1.贵州中医药大学药学院，贵阳 550025；2.海南医学院药学
1 #
院，海口 570100）
中图分类号 R285.5 文献标志码 A 文章编号 1001-0408（2020）18-2190-06
DOI 10.6039/j.issn.1001-0408.2020.18.03

摘 要 目的：探讨艳山姜提取物对急性胃溃疡模型小鼠的保护作用。方法：取小鼠48只，随机分为空白组、模型组、阳性组（三
九胃泰颗粒，20 mg/kg）和艳山姜提取物高、中、低剂量组（2.34、1.17、0.59 g/kg，按生药量计），每组8只，每天灌胃生理盐水或相应
药物1次，连续给药7 d后，除空白组外的其余组小鼠均一次性灌胃无水乙醇0.1 mL/10 g，以建立急性胃溃疡模型。另取小鼠48
只，同法分组，连续给药15 d，并于给药第10天起对除空白组外的其余组小鼠连续6 d灌胃阿司匹林（20 mg/kg），以建立急性胃溃
疡模型。在乙醇诱导模型实验中，观察各组小鼠胃溃疡形成情况并测定胃溃疡指数；采用苏木精-伊红染色法观察胃组织病理变
化；采用酶联免疫吸附法（ELISA）检测血清中胃泌素（GAS）和胃组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）的
水平。在阿司匹林诱导模型实验中，同法观察并测定各组小鼠胃溃疡形成情况、胃溃疡指数；采用ELISA法检测血清中GAS、肿
瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）和胃组织中前列腺素E2 （PGE2 ）、环氧合酶2（COX-2）的水平。结果：与空白组比较，
模型组小鼠胃组织均出现了明显的溃疡病变；血清中GAS、TNF-α、IL-1β和胃组织中MDA、NO水平均显著升高，胃组织中SOD、
PGE2、COX-2水平均显著降低（P＜0.05或P＜0.01）。与模型组比较，给药组小鼠的胃组织病变程度有不同程度减轻；胃溃疡指数
有不同程度降低，血清和胃组织中上述指标水平均有不同程度改善，大部分指标差异有统计学意义（P＜0.05或P＜0.01）。结论：艳山
姜提取物对无水乙醇和阿司匹林诱导的小鼠急性胃溃疡均有保护作用；其机制可能是通过抑制氧化应激和炎症反应，从而减轻胃黏
膜损伤。
关键词 艳山姜；提取物；急性胃溃疡；无水乙醇；阿司匹林；保护作用；小鼠

Study on the Protective Effect of Alpinia zerumbet Extract on Acute Gastric Ulcer Model Mice
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WEI Qing ，LIANG Shanshan ，XIONG Rui ，WEI Na ，XUE Juan（1. College of Pharmacy，Guizhou University
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of TCM，Guiyang 550025，China；2. College of Pharmacy，Hainan Medical University，Haikou 570100，
China）
ABSTRACT OBJECTIVE：To explore the protective effect of Alpinia zerumbet extract on acute gastric ulcer model mice.
METHODS：Totally 48 mice were collected and randomly divided into blank group，model group，positive group（Sanjiu weitai
granules，20 mg/kg），A. zerumbet extract high-dose，medium-dose and low-dose groups（2.34，1.17，0.59 g/kg，by crude drug），
with 8 mice in each group. They were given normal saline or relevant medicine intragastrically，once a day，for consecutive 7 d.
Then，except for blank group，other groups were given disposable intragastric administration of absolute ethanol 0.1 mL/10 g to
establish acute gastric ulcer model. Another 48 mice were collected and grouped with same method，and then given relevant
medicine for consecutive 15 d. From the 10th day of administration，except for blank group，other groups were given intragastric
administration of aspirin （20 mg/kg） for consecutive 6 d to establish acute gastric ulcer model. In ethanol induction model
experiment，the formation of gastric ulcer and the index of gastric ulcer were observed in each group；HE staining was used to
observe the pathological changes of gastric tissue；the levels of gastrin（GAS）in serum，and the levels of SOD，MDA and NO in
gastric tissue were determined by ELISA. In the aspirin induced model experiment，the formation of gastric ulcer and the index of
gastric ulcer were observed and measured by the same method；the levels of GAS，TNF-α，IL-1β in serum，and the levels of
PGE2 and COX-2 in gastric tissue were determined by ELISA. RESULTS：Compared with model group，obvious ulcer lesions were
found in gastric tissue of rats in model group；the levels of GAS，TNF-α，IL-1β in serum，and MDA，NO in gastric tissue were
increased significantly， while the levels of SOD， PGE2，
Δ 基金项目：国家自然科学基金资助项目（No.81660649）；贵州中
医药管理局中医药、民族医药科学技术研究课题（No.QZYY-2019- COX-2 in gastric tissue were decreased significantly（P＜0.05
or P＜0.01）. Compared with model group， the degree of
063）；贵州中医药大学博士启动基金（No.〔2019〕38）；贵州中医药大学
科研项目（No.贵中医科院内〔2019〕39号） gastric lesions in administration groups was alleviated to some
＊讲师，博士。研究方向：中药药效物质基础及作用机制。E- extent； gastric ulcer index was decreased to some extent，
mail：997845460@qq.com while the levels of the above indexes in serum and gastric
# 通信作者：讲师，博士。研究方向：中药药效物质基础及作用机 tissue were improved in varying degrees， with statistical
制。E-mail：672863283@qq.com significance in most indexes （P＜0.05 or P＜0.01）.

·2190 · China Pharmacy 2020 Vol. 31 No. 18 中国药房 2020年第31卷第18期