Page 59 - 2020年17期

不同产地茯苓中7种三萜类成分含量的测定及聚类分析 Δ

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万 鸣 ，黄 超 ，杨玉莹 ，罗心遥 ，叶晓川 ，丁 影 ，董 琼 ，陈树和 （1.湖北省中医院/湖北省中医药研
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究院药事部，武汉 430061；2.湖北中医药大学药学院，武汉 430065）
中图分类号 R284 文献标志码 A 文章编号 1001-0408（2020）17-2101-06
DOI 10.6039/j.issn.1001-0408.2020.17.10
摘 要 目的：建立同时测定茯苓药材中7种三萜类成分含量的方法，并比较不同产地茯苓药材中上述成分的差异性，为茯苓药
材的质量控制提供参考。方法：以不同产地的36批茯苓药材为样品，采用高效液相色谱法测定去氢土莫酸、猪苓酸C、3-表去氢土
莫酸、3-O-乙酰基-16α-羟基-氢化松苓酸、去氢茯苓酸、茯苓酸、松苓新酸的含量。色谱柱为Thermo Acclaim 120 C18，流动相为乙
腈-磷酸水（梯度洗脱），流速为1 mL/min，检测波长为210 nm，柱温为30 ℃，进样量为20 μL。采用SPSS 21.0统计学软件对36批
不同产地茯苓药材进行聚类分析。结果：7种三萜类成分在各自质量浓度范围内线性关系均良好（r均不低于0.999 0），平均加样
回收率为96.74％～104.04％（RSD为0.54％～1.55％，n＝6）；精密度、重复性、稳定性（24 h）试验的RSD均小于3.0％（n＝6）；耐用
性试验的RSD均小于5.0％（n＝2）。不同产地样品之间7个指标成分的单一含量均存在一定差异，但总体差异不明显（多数样品
总含量分布在1.3～1.9 mg/g之间）。经聚类分析，36批样品共聚为5类，即S27单独聚为第Ⅰ类，S30、S34聚为第Ⅱ类，S2、S8、S9
聚为第Ⅲ类，S10、S11、S12、S14聚为第Ⅳ类，其余26批样品聚为第Ⅴ类。结论：该方法操作简单，精密度、重复性、耐用性均良好，
可用于同时测定茯苓药材中上述7种三萜类成分的含量。各产地茯苓药材的质量总体差异不显著，大部分产区的茯苓含量均一、
质量稳定，仅少部分存在差异。
关键词 茯苓；三萜类成分；高效液相色谱法；含量测定；聚类分析

Content Determination and Cluster Analysis of 7 Kinds of Triterpenes in Poria cocos from Different
Habitats
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WAN Ming ，HUANG Chao ，YANG Yuying ，LUO Xinyao ，YE Xiaochuan ，DING Ying ，DONG Qiong ，
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CHEN Shuhe（1. Dept. of Pharmaceutical Affairs，Hubei Province Hospital of TCM/Hubei Academy of TCM，
Wuhan 430061，China；2. College of Pharmacy，Hubei University of TCM，Wuhan 430065，China）
ABSTRACT OBJECTIVE：To establish a method for the content determination of 7 kinds of triterpenoids in Poria cocos，and to
compare the differences of the above components in P. cocos from different habitats，so as to provide reference for the quality
control of P. cocos. METHODS：Using 36 batches of P. cocos from different habitats as samples，HPLC method was used for
content determination of dehydrotomorphic acid， polyporhinic acid C， 3-epidehydrotomorphic acid， 3-O-acetyl-16
α-hydroxy-hydrogenolysaccharic acid，dehydrotomorphic acid，pachymic acid and dehydrotrametenolic acid. The column was
performed on Thermo Acclaim 120 C18 with mobile phase consisted of acetonitrile-phosphoric acid water（gradient elution）at the
flow rate of 1 mL/min. The detection wavelength was set at 210 nm. The column temperature was 30 ℃，and the injection volume
was 20 μL. SPSS 21.0 statistical software was used for cluster analysis of 36 batches of P. cocos from different habitats. RESULTS：
The linearity of 7 triterpenoids was good in the range of their mass concentration （all r≥0.999 0）；average recoveries were
96.74％-104.04％（RSD＝0.54％-1.55％，n＝6）. RSDs of precision，and reproducibility stability（24 h）tests were all lower than
3.0％（n＝6）. RSD of durability test was lower than 5.0％（n＝2）. There were some differences in the single content of 7 indicator
components among samples from different habitats，but the total content difference was not obvious（the total content of most
samples was in the range of 1.3-1.9 mg/g）. After cluster analysis，36 batches of sample were clustered into 5 categories，i.e. S27
was clustered into class Ⅰ；S30 and S34 were clustered into class Ⅱ；S2，S8 and S9 were clustered into class Ⅲ；S10，S11，S12
and S14 were clustered into class Ⅳ；and the remaining 26 batches of samples were clustered into class Ⅴ. CONCLUSIONS：The
method is simple，and has good precision，repeatability and durability. It can be used for the simultaneous determination of above 7
components in P. cocos. There has no significant difference in the quality of P. cocos from different habitats. The content of P. cocos
in most producing areas is uniform in content and stable in quality，only a few of them are different.
KEYWORDS Poria cocos；Triterpenoids；HPLC；Content
Δ 基金项目：国家重点研发计划中医药现代化研究重点专项
determination；Cluster analysis
（No.2017YFC1703000）；湖北省技术创新专项（No.2019ACA121）
＊ 副 研 究 员 。 研 究 方 向 ：中 药 制 剂 及 质 量 控 制 。 E-mail：
15827273808@139.com
# 通信作者：主任药师。研究方向：中药制剂及其物质基础。E- 茯 苓 为 多 孔 菌 科 真 菌 茯 苓 [Poria cocos（Schw.）
mail：chenshuhe606@163.com Wolf]的干燥菌核，始载于《神农本草经》，列为上品，其

中国药房 2020年第31卷第17期 China Pharmacy 2020 Vol. 31 No. 17 ·2101 ·